Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate

Detalhes bibliográficos
Autor(a) principal: de Castilho, Aline R. F.
Data de Publicação: 2012
Outros Autores: Duque, Cristiane, Negrini, Thais de C. [UNESP], Sacono, Nancy T. [UNESP], de Paula, Andreia B., Sacramento, Patricia A., de Souza Costal, Carlos A. [UNESP], Spolidorio, Denise M. P. [UNESP], Puppin-Rontani, Regina M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2011.08.009
http://hdl.handle.net/11449/15804
Resumo: Objectives: To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline'hyclate.Methods: The antibacterial effect of RMGIC containing 1.5, 3.0 and 4.5% doxycycline hyclate was assessed using two experiments - agar diffusion test for 24 h and biofilm assay for 24 h and 7 days - against some cariogenic bacteria. Briefly, base layers of BHI agar and 300 mu L of each inoculum were prepared in Petri dishes with 6 wells that were completely filled with materials. After 24 h incubation, zones of bacterial growth inhibition were measured using a digital caliper. Biofilm assays were conducted using RMGIC specimens immersed in 24-well plates containing the inoculum in BHI broth. After 24 h and 7 days, each specimen were removed, vortexed and the suspension diluted and inoculated in BHI plates for subsequent bacterial counting. Cytotoxicity tests used 50 specimens made in sterilized metal molds, including Vitrebond as positive control. Extracts from every specimen were applied on the MDPC-23 odontoblast-like cells for 24 h. The MIT assay and SEM evaluation determined cell metabolism and morphology, respectively. 80 cylindrical specimens were made from the previously cited groups, and were submitted to testing with a universal testing machine (Instron 4411) using a crosshead speed of 1.0 mm/min for compressive strength and 0.5 mm/mm for diametral tensile strength, respectively. Data from antibacterial and cytotoxic effects, and mechanical properties were submitted to appropriated statistical tests.Results: All tested groups showed growth inhibition of all tested strains (p < 0.05) in 24 h for both microbiological tests, but only 4.5% doxycycline have antibacterial effect after 7 days. None of doxycycline concentrations caused toxic effect to the MDPC-23 cells or presenting alterations to mechanical properties.Conclusion: The incorporation of up to 4.5% doxycycline hyclate into RMGIC inhibits important oral microorganisms, without modifying biological and mechanical characteristics of the dental material, suggesting a new alternative for the treatment of dental caries. (C) 2011 Elsevier Ltd. All rights reserved.
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spelling Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclateAntibioticGlass-ionomer cementAntibacterial activityCell cultureMechanical propertiesObjectives: To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline'hyclate.Methods: The antibacterial effect of RMGIC containing 1.5, 3.0 and 4.5% doxycycline hyclate was assessed using two experiments - agar diffusion test for 24 h and biofilm assay for 24 h and 7 days - against some cariogenic bacteria. Briefly, base layers of BHI agar and 300 mu L of each inoculum were prepared in Petri dishes with 6 wells that were completely filled with materials. After 24 h incubation, zones of bacterial growth inhibition were measured using a digital caliper. Biofilm assays were conducted using RMGIC specimens immersed in 24-well plates containing the inoculum in BHI broth. After 24 h and 7 days, each specimen were removed, vortexed and the suspension diluted and inoculated in BHI plates for subsequent bacterial counting. Cytotoxicity tests used 50 specimens made in sterilized metal molds, including Vitrebond as positive control. Extracts from every specimen were applied on the MDPC-23 odontoblast-like cells for 24 h. The MIT assay and SEM evaluation determined cell metabolism and morphology, respectively. 80 cylindrical specimens were made from the previously cited groups, and were submitted to testing with a universal testing machine (Instron 4411) using a crosshead speed of 1.0 mm/min for compressive strength and 0.5 mm/mm for diametral tensile strength, respectively. Data from antibacterial and cytotoxic effects, and mechanical properties were submitted to appropriated statistical tests.Results: All tested groups showed growth inhibition of all tested strains (p < 0.05) in 24 h for both microbiological tests, but only 4.5% doxycycline have antibacterial effect after 7 days. None of doxycycline concentrations caused toxic effect to the MDPC-23 cells or presenting alterations to mechanical properties.Conclusion: The incorporation of up to 4.5% doxycycline hyclate into RMGIC inhibits important oral microorganisms, without modifying biological and mechanical characteristics of the dental material, suggesting a new alternative for the treatment of dental caries. (C) 2011 Elsevier Ltd. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed Fluminense, Dept Dent, BR-28625650 Rio de Janeiro, BrazilUniv Campinas UNICAMP, Piracicaba Dent Sch, Dept Pediat Dent, BR-13414903 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Pharmaceut Sci, Dept Clin Anal, BR-14801902 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 São Paulo, BrazilUniv Campinas UNICAMP, Piracicaba Dent Sch, Dept Dent Mat, BR-13414903 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Pharmaceut Sci, Dept Clin Anal, BR-14801902 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 São Paulo, BrazilFAPESP: 08/00359-0FAPESP: 08/02606-5Pergamon-Elsevier B.V. LtdUniversidade Federal Fluminense (UFF)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)de Castilho, Aline R. F.Duque, CristianeNegrini, Thais de C. [UNESP]Sacono, Nancy T. [UNESP]de Paula, Andreia B.Sacramento, Patricia A.de Souza Costal, Carlos A. [UNESP]Spolidorio, Denise M. P. [UNESP]Puppin-Rontani, Regina M.2013-09-30T18:31:37Z2014-05-20T13:45:02Z2013-09-30T18:31:37Z2014-05-20T13:45:02Z2012-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article131-138application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2011.08.009Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 131-138, 2012.0003-9969http://hdl.handle.net/11449/1580410.1016/j.archoralbio.2011.08.009WOS:000300801900003WOS000300801900003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology2.0500,752info:eu-repo/semantics/openAccess2024-09-27T14:05:25Zoai:repositorio.unesp.br:11449/15804Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate
title Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate
spellingShingle Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate
de Castilho, Aline R. F.
Antibiotic
Glass-ionomer cement
Antibacterial activity
Cell culture
Mechanical properties
title_short Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate
title_full Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate
title_fullStr Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate
title_full_unstemmed Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate
title_sort Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate
author de Castilho, Aline R. F.
author_facet de Castilho, Aline R. F.
Duque, Cristiane
Negrini, Thais de C. [UNESP]
Sacono, Nancy T. [UNESP]
de Paula, Andreia B.
Sacramento, Patricia A.
de Souza Costal, Carlos A. [UNESP]
Spolidorio, Denise M. P. [UNESP]
Puppin-Rontani, Regina M.
author_role author
author2 Duque, Cristiane
Negrini, Thais de C. [UNESP]
Sacono, Nancy T. [UNESP]
de Paula, Andreia B.
Sacramento, Patricia A.
de Souza Costal, Carlos A. [UNESP]
Spolidorio, Denise M. P. [UNESP]
Puppin-Rontani, Regina M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal Fluminense (UFF)
Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv de Castilho, Aline R. F.
Duque, Cristiane
Negrini, Thais de C. [UNESP]
Sacono, Nancy T. [UNESP]
de Paula, Andreia B.
Sacramento, Patricia A.
de Souza Costal, Carlos A. [UNESP]
Spolidorio, Denise M. P. [UNESP]
Puppin-Rontani, Regina M.
dc.subject.por.fl_str_mv Antibiotic
Glass-ionomer cement
Antibacterial activity
Cell culture
Mechanical properties
topic Antibiotic
Glass-ionomer cement
Antibacterial activity
Cell culture
Mechanical properties
description Objectives: To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline'hyclate.Methods: The antibacterial effect of RMGIC containing 1.5, 3.0 and 4.5% doxycycline hyclate was assessed using two experiments - agar diffusion test for 24 h and biofilm assay for 24 h and 7 days - against some cariogenic bacteria. Briefly, base layers of BHI agar and 300 mu L of each inoculum were prepared in Petri dishes with 6 wells that were completely filled with materials. After 24 h incubation, zones of bacterial growth inhibition were measured using a digital caliper. Biofilm assays were conducted using RMGIC specimens immersed in 24-well plates containing the inoculum in BHI broth. After 24 h and 7 days, each specimen were removed, vortexed and the suspension diluted and inoculated in BHI plates for subsequent bacterial counting. Cytotoxicity tests used 50 specimens made in sterilized metal molds, including Vitrebond as positive control. Extracts from every specimen were applied on the MDPC-23 odontoblast-like cells for 24 h. The MIT assay and SEM evaluation determined cell metabolism and morphology, respectively. 80 cylindrical specimens were made from the previously cited groups, and were submitted to testing with a universal testing machine (Instron 4411) using a crosshead speed of 1.0 mm/min for compressive strength and 0.5 mm/mm for diametral tensile strength, respectively. Data from antibacterial and cytotoxic effects, and mechanical properties were submitted to appropriated statistical tests.Results: All tested groups showed growth inhibition of all tested strains (p < 0.05) in 24 h for both microbiological tests, but only 4.5% doxycycline have antibacterial effect after 7 days. None of doxycycline concentrations caused toxic effect to the MDPC-23 cells or presenting alterations to mechanical properties.Conclusion: The incorporation of up to 4.5% doxycycline hyclate into RMGIC inhibits important oral microorganisms, without modifying biological and mechanical characteristics of the dental material, suggesting a new alternative for the treatment of dental caries. (C) 2011 Elsevier Ltd. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-01
2013-09-30T18:31:37Z
2013-09-30T18:31:37Z
2014-05-20T13:45:02Z
2014-05-20T13:45:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2011.08.009
Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 131-138, 2012.
0003-9969
http://hdl.handle.net/11449/15804
10.1016/j.archoralbio.2011.08.009
WOS:000300801900003
WOS000300801900003.pdf
url http://dx.doi.org/10.1016/j.archoralbio.2011.08.009
http://hdl.handle.net/11449/15804
identifier_str_mv Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 131-138, 2012.
0003-9969
10.1016/j.archoralbio.2011.08.009
WOS:000300801900003
WOS000300801900003.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
2.050
0,752
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 131-138
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier B.V. Ltd
publisher.none.fl_str_mv Pergamon-Elsevier B.V. Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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