Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2011.08.009 http://hdl.handle.net/11449/15804 |
Resumo: | Objectives: To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline'hyclate.Methods: The antibacterial effect of RMGIC containing 1.5, 3.0 and 4.5% doxycycline hyclate was assessed using two experiments - agar diffusion test for 24 h and biofilm assay for 24 h and 7 days - against some cariogenic bacteria. Briefly, base layers of BHI agar and 300 mu L of each inoculum were prepared in Petri dishes with 6 wells that were completely filled with materials. After 24 h incubation, zones of bacterial growth inhibition were measured using a digital caliper. Biofilm assays were conducted using RMGIC specimens immersed in 24-well plates containing the inoculum in BHI broth. After 24 h and 7 days, each specimen were removed, vortexed and the suspension diluted and inoculated in BHI plates for subsequent bacterial counting. Cytotoxicity tests used 50 specimens made in sterilized metal molds, including Vitrebond as positive control. Extracts from every specimen were applied on the MDPC-23 odontoblast-like cells for 24 h. The MIT assay and SEM evaluation determined cell metabolism and morphology, respectively. 80 cylindrical specimens were made from the previously cited groups, and were submitted to testing with a universal testing machine (Instron 4411) using a crosshead speed of 1.0 mm/min for compressive strength and 0.5 mm/mm for diametral tensile strength, respectively. Data from antibacterial and cytotoxic effects, and mechanical properties were submitted to appropriated statistical tests.Results: All tested groups showed growth inhibition of all tested strains (p < 0.05) in 24 h for both microbiological tests, but only 4.5% doxycycline have antibacterial effect after 7 days. None of doxycycline concentrations caused toxic effect to the MDPC-23 cells or presenting alterations to mechanical properties.Conclusion: The incorporation of up to 4.5% doxycycline hyclate into RMGIC inhibits important oral microorganisms, without modifying biological and mechanical characteristics of the dental material, suggesting a new alternative for the treatment of dental caries. (C) 2011 Elsevier Ltd. All rights reserved. |
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Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclateAntibioticGlass-ionomer cementAntibacterial activityCell cultureMechanical propertiesObjectives: To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline'hyclate.Methods: The antibacterial effect of RMGIC containing 1.5, 3.0 and 4.5% doxycycline hyclate was assessed using two experiments - agar diffusion test for 24 h and biofilm assay for 24 h and 7 days - against some cariogenic bacteria. Briefly, base layers of BHI agar and 300 mu L of each inoculum were prepared in Petri dishes with 6 wells that were completely filled with materials. After 24 h incubation, zones of bacterial growth inhibition were measured using a digital caliper. Biofilm assays were conducted using RMGIC specimens immersed in 24-well plates containing the inoculum in BHI broth. After 24 h and 7 days, each specimen were removed, vortexed and the suspension diluted and inoculated in BHI plates for subsequent bacterial counting. Cytotoxicity tests used 50 specimens made in sterilized metal molds, including Vitrebond as positive control. Extracts from every specimen were applied on the MDPC-23 odontoblast-like cells for 24 h. The MIT assay and SEM evaluation determined cell metabolism and morphology, respectively. 80 cylindrical specimens were made from the previously cited groups, and were submitted to testing with a universal testing machine (Instron 4411) using a crosshead speed of 1.0 mm/min for compressive strength and 0.5 mm/mm for diametral tensile strength, respectively. Data from antibacterial and cytotoxic effects, and mechanical properties were submitted to appropriated statistical tests.Results: All tested groups showed growth inhibition of all tested strains (p < 0.05) in 24 h for both microbiological tests, but only 4.5% doxycycline have antibacterial effect after 7 days. None of doxycycline concentrations caused toxic effect to the MDPC-23 cells or presenting alterations to mechanical properties.Conclusion: The incorporation of up to 4.5% doxycycline hyclate into RMGIC inhibits important oral microorganisms, without modifying biological and mechanical characteristics of the dental material, suggesting a new alternative for the treatment of dental caries. (C) 2011 Elsevier Ltd. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed Fluminense, Dept Dent, BR-28625650 Rio de Janeiro, BrazilUniv Campinas UNICAMP, Piracicaba Dent Sch, Dept Pediat Dent, BR-13414903 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Pharmaceut Sci, Dept Clin Anal, BR-14801902 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 São Paulo, BrazilUniv Campinas UNICAMP, Piracicaba Dent Sch, Dept Dent Mat, BR-13414903 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Pharmaceut Sci, Dept Clin Anal, BR-14801902 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-14801903 São Paulo, BrazilUniv Estadual Paulista UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, BR-14801903 São Paulo, BrazilFAPESP: 08/00359-0FAPESP: 08/02606-5Pergamon-Elsevier B.V. LtdUniversidade Federal Fluminense (UFF)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)de Castilho, Aline R. F.Duque, CristianeNegrini, Thais de C. [UNESP]Sacono, Nancy T. [UNESP]de Paula, Andreia B.Sacramento, Patricia A.de Souza Costal, Carlos A. [UNESP]Spolidorio, Denise M. P. [UNESP]Puppin-Rontani, Regina M.2013-09-30T18:31:37Z2014-05-20T13:45:02Z2013-09-30T18:31:37Z2014-05-20T13:45:02Z2012-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article131-138application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2011.08.009Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 131-138, 2012.0003-9969http://hdl.handle.net/11449/1580410.1016/j.archoralbio.2011.08.009WOS:000300801900003WOS000300801900003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology2.0500,752info:eu-repo/semantics/openAccess2024-09-27T14:05:25Zoai:repositorio.unesp.br:11449/15804Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:05:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate |
title |
Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate |
spellingShingle |
Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate de Castilho, Aline R. F. Antibiotic Glass-ionomer cement Antibacterial activity Cell culture Mechanical properties |
title_short |
Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate |
title_full |
Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate |
title_fullStr |
Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate |
title_full_unstemmed |
Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate |
title_sort |
Mechanical and biological characterization of resin-modified glass-ionomer cement containing doxycycline hyclate |
author |
de Castilho, Aline R. F. |
author_facet |
de Castilho, Aline R. F. Duque, Cristiane Negrini, Thais de C. [UNESP] Sacono, Nancy T. [UNESP] de Paula, Andreia B. Sacramento, Patricia A. de Souza Costal, Carlos A. [UNESP] Spolidorio, Denise M. P. [UNESP] Puppin-Rontani, Regina M. |
author_role |
author |
author2 |
Duque, Cristiane Negrini, Thais de C. [UNESP] Sacono, Nancy T. [UNESP] de Paula, Andreia B. Sacramento, Patricia A. de Souza Costal, Carlos A. [UNESP] Spolidorio, Denise M. P. [UNESP] Puppin-Rontani, Regina M. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal Fluminense (UFF) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Castilho, Aline R. F. Duque, Cristiane Negrini, Thais de C. [UNESP] Sacono, Nancy T. [UNESP] de Paula, Andreia B. Sacramento, Patricia A. de Souza Costal, Carlos A. [UNESP] Spolidorio, Denise M. P. [UNESP] Puppin-Rontani, Regina M. |
dc.subject.por.fl_str_mv |
Antibiotic Glass-ionomer cement Antibacterial activity Cell culture Mechanical properties |
topic |
Antibiotic Glass-ionomer cement Antibacterial activity Cell culture Mechanical properties |
description |
Objectives: To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline'hyclate.Methods: The antibacterial effect of RMGIC containing 1.5, 3.0 and 4.5% doxycycline hyclate was assessed using two experiments - agar diffusion test for 24 h and biofilm assay for 24 h and 7 days - against some cariogenic bacteria. Briefly, base layers of BHI agar and 300 mu L of each inoculum were prepared in Petri dishes with 6 wells that were completely filled with materials. After 24 h incubation, zones of bacterial growth inhibition were measured using a digital caliper. Biofilm assays were conducted using RMGIC specimens immersed in 24-well plates containing the inoculum in BHI broth. After 24 h and 7 days, each specimen were removed, vortexed and the suspension diluted and inoculated in BHI plates for subsequent bacterial counting. Cytotoxicity tests used 50 specimens made in sterilized metal molds, including Vitrebond as positive control. Extracts from every specimen were applied on the MDPC-23 odontoblast-like cells for 24 h. The MIT assay and SEM evaluation determined cell metabolism and morphology, respectively. 80 cylindrical specimens were made from the previously cited groups, and were submitted to testing with a universal testing machine (Instron 4411) using a crosshead speed of 1.0 mm/min for compressive strength and 0.5 mm/mm for diametral tensile strength, respectively. Data from antibacterial and cytotoxic effects, and mechanical properties were submitted to appropriated statistical tests.Results: All tested groups showed growth inhibition of all tested strains (p < 0.05) in 24 h for both microbiological tests, but only 4.5% doxycycline have antibacterial effect after 7 days. None of doxycycline concentrations caused toxic effect to the MDPC-23 cells or presenting alterations to mechanical properties.Conclusion: The incorporation of up to 4.5% doxycycline hyclate into RMGIC inhibits important oral microorganisms, without modifying biological and mechanical characteristics of the dental material, suggesting a new alternative for the treatment of dental caries. (C) 2011 Elsevier Ltd. All rights reserved. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-01 2013-09-30T18:31:37Z 2013-09-30T18:31:37Z 2014-05-20T13:45:02Z 2014-05-20T13:45:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2011.08.009 Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 131-138, 2012. 0003-9969 http://hdl.handle.net/11449/15804 10.1016/j.archoralbio.2011.08.009 WOS:000300801900003 WOS000300801900003.pdf |
url |
http://dx.doi.org/10.1016/j.archoralbio.2011.08.009 http://hdl.handle.net/11449/15804 |
identifier_str_mv |
Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 57, n. 2, p. 131-138, 2012. 0003-9969 10.1016/j.archoralbio.2011.08.009 WOS:000300801900003 WOS000300801900003.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 2.050 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
131-138 application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier B.V. Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier B.V. Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546461487431680 |