Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jemt.22814 http://hdl.handle.net/11449/231391 |
Resumo: | This study evaluated the antibacterial activity of zinc oxide nanoparticles incorporated into self-cured glass ionomer cement (GIC) and light-cured resin-reinforced GIC on Streptococcus mutans biofilm. The GICs, Fuji II (GC America) and Fuji II LC (GC America), were incorporated with nanoparticles at concentrations of 0%, 1%, and 2% by weight, and the biofilm maturation time was one and seven days. Circular specimens of each GIC type were prepared. The antibacterial activity was evaluated by determining the number of colony forming units of S. mutans strain per milliliter. Morphology of the biofilm was analyzed by scanning electron microscopy (SEM). The data obtained for each GIC were analyzed by two-way ANOVA (α = 5%). For chemically activated GIC, no significant difference was observed in relation to the time of biofilm maturation (p = 0.744), concentration of nanoparticles (p = 0.966), and their interaction (p = 0.800). The results from analysis of GIC modified by light-polymerized resin showed that only of the maturing time significantly affected the number of adhered cells on the biofilm (p = 0.034, F = 4.778). The more mature the biofilm, higher the number of cells. SEM analysis showed no change in cell morphology in relation to the type of GIC, maturation time, and nanoparticles concentration. We conclude that the inclusion of zinc oxide nanoparticles at concentrations of 1% and 2% by weight into the GICs evaluated here, did not promote their antimicrobial activity against S. mutans. |
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Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticlesantibacterialbiofilmsglass ionomer cementmicroscopynanoparticlesSEMzinc oxideThis study evaluated the antibacterial activity of zinc oxide nanoparticles incorporated into self-cured glass ionomer cement (GIC) and light-cured resin-reinforced GIC on Streptococcus mutans biofilm. The GICs, Fuji II (GC America) and Fuji II LC (GC America), were incorporated with nanoparticles at concentrations of 0%, 1%, and 2% by weight, and the biofilm maturation time was one and seven days. Circular specimens of each GIC type were prepared. The antibacterial activity was evaluated by determining the number of colony forming units of S. mutans strain per milliliter. Morphology of the biofilm was analyzed by scanning electron microscopy (SEM). The data obtained for each GIC were analyzed by two-way ANOVA (α = 5%). For chemically activated GIC, no significant difference was observed in relation to the time of biofilm maturation (p = 0.744), concentration of nanoparticles (p = 0.966), and their interaction (p = 0.800). The results from analysis of GIC modified by light-polymerized resin showed that only of the maturing time significantly affected the number of adhered cells on the biofilm (p = 0.034, F = 4.778). The more mature the biofilm, higher the number of cells. SEM analysis showed no change in cell morphology in relation to the type of GIC, maturation time, and nanoparticles concentration. We conclude that the inclusion of zinc oxide nanoparticles at concentrations of 1% and 2% by weight into the GICs evaluated here, did not promote their antimicrobial activity against S. mutans.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Social Dentistry Araraquara School of Dentistry University of São Paulo StateDepartment of Physiology and Pathology Araraquara School of Dentistry University of São Paulo StateDepartment of Restorative Dentistry Araraquara School of Dentistry University of São Paulo StateDepartment of General Dentistry Stony Brook School of Dental MedicineUniversidade de São Paulo (USP)Stony Brook School of Dental MedicineGarcia, Patrícia Petromilli Nordi SassoCardia, Mariana Florian BellFrancisconi, Renata SerignoliDovigo, Lívia NordiSpolidório, Denise Madalena Palomaride Souza Rastelli, Alessandra NaraBotta, Ana Carolina2022-04-29T08:45:03Z2022-04-29T08:45:03Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article456-461http://dx.doi.org/10.1002/jemt.22814Microscopy Research and Technique, v. 80, n. 5, p. 456-461, 2017.1097-00291059-910Xhttp://hdl.handle.net/11449/23139110.1002/jemt.228142-s2.0-85006713608Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroscopy Research and Techniqueinfo:eu-repo/semantics/openAccess2022-04-29T08:45:03Zoai:repositorio.unesp.br:11449/231391Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:45:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles |
title |
Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles |
spellingShingle |
Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles Garcia, Patrícia Petromilli Nordi Sasso antibacterial biofilms glass ionomer cement microscopy nanoparticles SEM zinc oxide |
title_short |
Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles |
title_full |
Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles |
title_fullStr |
Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles |
title_full_unstemmed |
Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles |
title_sort |
Antibacterial activity of glass ionomer cement modified by zinc oxide nanoparticles |
author |
Garcia, Patrícia Petromilli Nordi Sasso |
author_facet |
Garcia, Patrícia Petromilli Nordi Sasso Cardia, Mariana Florian Bell Francisconi, Renata Serignoli Dovigo, Lívia Nordi Spolidório, Denise Madalena Palomari de Souza Rastelli, Alessandra Nara Botta, Ana Carolina |
author_role |
author |
author2 |
Cardia, Mariana Florian Bell Francisconi, Renata Serignoli Dovigo, Lívia Nordi Spolidório, Denise Madalena Palomari de Souza Rastelli, Alessandra Nara Botta, Ana Carolina |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Stony Brook School of Dental Medicine |
dc.contributor.author.fl_str_mv |
Garcia, Patrícia Petromilli Nordi Sasso Cardia, Mariana Florian Bell Francisconi, Renata Serignoli Dovigo, Lívia Nordi Spolidório, Denise Madalena Palomari de Souza Rastelli, Alessandra Nara Botta, Ana Carolina |
dc.subject.por.fl_str_mv |
antibacterial biofilms glass ionomer cement microscopy nanoparticles SEM zinc oxide |
topic |
antibacterial biofilms glass ionomer cement microscopy nanoparticles SEM zinc oxide |
description |
This study evaluated the antibacterial activity of zinc oxide nanoparticles incorporated into self-cured glass ionomer cement (GIC) and light-cured resin-reinforced GIC on Streptococcus mutans biofilm. The GICs, Fuji II (GC America) and Fuji II LC (GC America), were incorporated with nanoparticles at concentrations of 0%, 1%, and 2% by weight, and the biofilm maturation time was one and seven days. Circular specimens of each GIC type were prepared. The antibacterial activity was evaluated by determining the number of colony forming units of S. mutans strain per milliliter. Morphology of the biofilm was analyzed by scanning electron microscopy (SEM). The data obtained for each GIC were analyzed by two-way ANOVA (α = 5%). For chemically activated GIC, no significant difference was observed in relation to the time of biofilm maturation (p = 0.744), concentration of nanoparticles (p = 0.966), and their interaction (p = 0.800). The results from analysis of GIC modified by light-polymerized resin showed that only of the maturing time significantly affected the number of adhered cells on the biofilm (p = 0.034, F = 4.778). The more mature the biofilm, higher the number of cells. SEM analysis showed no change in cell morphology in relation to the type of GIC, maturation time, and nanoparticles concentration. We conclude that the inclusion of zinc oxide nanoparticles at concentrations of 1% and 2% by weight into the GICs evaluated here, did not promote their antimicrobial activity against S. mutans. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 2022-04-29T08:45:03Z 2022-04-29T08:45:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jemt.22814 Microscopy Research and Technique, v. 80, n. 5, p. 456-461, 2017. 1097-0029 1059-910X http://hdl.handle.net/11449/231391 10.1002/jemt.22814 2-s2.0-85006713608 |
url |
http://dx.doi.org/10.1002/jemt.22814 http://hdl.handle.net/11449/231391 |
identifier_str_mv |
Microscopy Research and Technique, v. 80, n. 5, p. 456-461, 2017. 1097-0029 1059-910X 10.1002/jemt.22814 2-s2.0-85006713608 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microscopy Research and Technique |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
456-461 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964448055623680 |