Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry

Detalhes bibliográficos
Autor(a) principal: Rall, VLM
Data de Publicação: 2005
Outros Autores: Rall, R., Aragon, L. C., Silva, M. G. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/195844
Resumo: We evaluated the effectiveness of Selenite Cystine (SC), Tetrathionate Brilliant Green (TBG) and Rappaport Vassiliadis (RV) broths for Salmonella isolation. We also tested three classic plating media, Salmonella-Shigella-Agar (SS), Brilliant Green Agar (BGA), Xylose Lysine Desoxycholate Agar (XLD) and two chromogenic agars, Rambach (RA) and CHROMagar Salmonella (CAS). Among 100 poultry carcasses, 29 were positive for Salmonella using all plating media combined. RV broth (69%) and TT broth (58.6%) were more effective than SC broth (24.1%). The chromogenic media gave better results than the classic ones with less false-positive colonies. The most effective isolation medium was CHROMagar, where Salmonella was identified in 23 (79.3%) of the 29 positive samples, followed by Rambach (48%). Positivity for Salmonella using classic media was 13.8% for BGA, 27.6% for SS and 34.5% for XLD.
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spelling Evaluation of three enrichment broths and five plating media for Salmonella detection in poultrySalmonellapoultryplating mediaselective enrichmentWe evaluated the effectiveness of Selenite Cystine (SC), Tetrathionate Brilliant Green (TBG) and Rappaport Vassiliadis (RV) broths for Salmonella isolation. We also tested three classic plating media, Salmonella-Shigella-Agar (SS), Brilliant Green Agar (BGA), Xylose Lysine Desoxycholate Agar (XLD) and two chromogenic agars, Rambach (RA) and CHROMagar Salmonella (CAS). Among 100 poultry carcasses, 29 were positive for Salmonella using all plating media combined. RV broth (69%) and TT broth (58.6%) were more effective than SC broth (24.1%). The chromogenic media gave better results than the classic ones with less false-positive colonies. The most effective isolation medium was CHROMagar, where Salmonella was identified in 23 (79.3%) of the 29 positive samples, followed by Rambach (48%). Positivity for Salmonella using classic media was 13.8% for BGA, 27.6% for SS and 34.5% for XLD.Univ Estadual Paulista, Inst Biociencias, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Patol, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Patol, Botucatu, SP, BrazilSoc Brasileira MicrobiologiaUniversidade Estadual Paulista (Unesp)Rall, VLMRall, R.Aragon, L. C.Silva, M. G. da2020-12-10T18:05:13Z2020-12-10T18:05:13Z2005-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article147-150Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 36, n. 2, p. 147-150, 2005.1517-8382http://hdl.handle.net/11449/195844WOS:000234802900009Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Microbiologyinfo:eu-repo/semantics/openAccess2024-09-03T13:18:23Zoai:repositorio.unesp.br:11449/195844Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
title Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
spellingShingle Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
Rall, VLM
Salmonella
poultry
plating media
selective enrichment
title_short Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
title_full Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
title_fullStr Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
title_full_unstemmed Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
title_sort Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry
author Rall, VLM
author_facet Rall, VLM
Rall, R.
Aragon, L. C.
Silva, M. G. da
author_role author
author2 Rall, R.
Aragon, L. C.
Silva, M. G. da
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rall, VLM
Rall, R.
Aragon, L. C.
Silva, M. G. da
dc.subject.por.fl_str_mv Salmonella
poultry
plating media
selective enrichment
topic Salmonella
poultry
plating media
selective enrichment
description We evaluated the effectiveness of Selenite Cystine (SC), Tetrathionate Brilliant Green (TBG) and Rappaport Vassiliadis (RV) broths for Salmonella isolation. We also tested three classic plating media, Salmonella-Shigella-Agar (SS), Brilliant Green Agar (BGA), Xylose Lysine Desoxycholate Agar (XLD) and two chromogenic agars, Rambach (RA) and CHROMagar Salmonella (CAS). Among 100 poultry carcasses, 29 were positive for Salmonella using all plating media combined. RV broth (69%) and TT broth (58.6%) were more effective than SC broth (24.1%). The chromogenic media gave better results than the classic ones with less false-positive colonies. The most effective isolation medium was CHROMagar, where Salmonella was identified in 23 (79.3%) of the 29 positive samples, followed by Rambach (48%). Positivity for Salmonella using classic media was 13.8% for BGA, 27.6% for SS and 34.5% for XLD.
publishDate 2005
dc.date.none.fl_str_mv 2005-04-01
2020-12-10T18:05:13Z
2020-12-10T18:05:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 36, n. 2, p. 147-150, 2005.
1517-8382
http://hdl.handle.net/11449/195844
WOS:000234802900009
identifier_str_mv Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 36, n. 2, p. 147-150, 2005.
1517-8382
WOS:000234802900009
url http://hdl.handle.net/11449/195844
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal Of Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 147-150
dc.publisher.none.fl_str_mv Soc Brasileira Microbiologia
publisher.none.fl_str_mv Soc Brasileira Microbiologia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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