Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interaction

Detalhes bibliográficos
Autor(a) principal: Marcos, Caroline Maria [UNESP]
Data de Publicação: 2016
Outros Autores: de Oliveira, Haroldo Cesar [UNESP], da Silva, Julhiany de Fátima [UNESP], Assato, Patricia Akemi [UNESP], Yamazaki, Daniella Sayuri [UNESP], da Silva, Rosângela Aparecida Moraes [UNESP], Santos, Cláudia Tavares [UNESP], Santos-Filho, Norival Alves [UNESP], Portuondo, Deivys Leandro [UNESP], Mendes-Giannini, Maria José Soares [UNESP], Fusco-Almeida, Ana Marisa [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1093/femsyr/fow079
http://hdl.handle.net/11449/173769
Resumo: Paracoccidioides spp., which are temperature-dependent dimorphic fungi, are responsible for the most prevalent human systemic mycosis in Latin America, the paracoccidioidomycosis. The aim of this study was to characterise the involvement of elongation factor Tu (EF-Tu) in Paracoccidioides brasiliensis-host interaction. Adhesive properties were examined using recombinant PbEF-Tu proteins and the respective polyclonal anti-rPbEF-Tu antibody. Immunogold analysis demonstrated the surface location of EF-Tu in P. brasiliensis. Moreover, PbEF-Tu was found to bind to fibronectin and plasminogen by enzyme-linked immunosorbent assay, and it was determined that the binding to plasminogen is at least partly dependent on lysine residues and ionic interactions. To verify the participation of EF-Tu in the interaction of P. brasiliensis with pneumocytes, we blocked the respective protein with an anti-rPbEF-Tu antibody and evaluated the consequences on the interaction index by flow cytometry. During the interaction, we observed a decrease of 2- and 3-fold at 8 and 24 h, respectively, suggesting the contribution of EF-Tu in fungal adhesion/invasion.
id UNSP_776ccee2ec7e68e2ba1e3d31f7810d0e
oai_identifier_str oai:repositorio.unesp.br:11449/173769
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interactionEF-Tufibronectinfungal-host interactionmoonlighting proteinParacoccidioides brasiliensisplasminogenParacoccidioides spp., which are temperature-dependent dimorphic fungi, are responsible for the most prevalent human systemic mycosis in Latin America, the paracoccidioidomycosis. The aim of this study was to characterise the involvement of elongation factor Tu (EF-Tu) in Paracoccidioides brasiliensis-host interaction. Adhesive properties were examined using recombinant PbEF-Tu proteins and the respective polyclonal anti-rPbEF-Tu antibody. Immunogold analysis demonstrated the surface location of EF-Tu in P. brasiliensis. Moreover, PbEF-Tu was found to bind to fibronectin and plasminogen by enzyme-linked immunosorbent assay, and it was determined that the binding to plasminogen is at least partly dependent on lysine residues and ionic interactions. To verify the participation of EF-Tu in the interaction of P. brasiliensis with pneumocytes, we blocked the respective protein with an anti-rPbEF-Tu antibody and evaluated the consequences on the interaction index by flow cytometry. During the interaction, we observed a decrease of 2- and 3-fold at 8 and 24 h, respectively, suggesting the contribution of EF-Tu in fungal adhesion/invasion.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculdade de Ciências Farmacêuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Micologia ClínicaInstituto de Química UNESP - Univ Estadual Paulista Departamento de Bioquímica e Tecnologia Química Unidade de Sintese Estrutura e Caracterizacao de Peptideos e ProteinasFaculdade de Ciências Farmacéuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Imunologia ClínicaFaculdade de Ciências Farmacêuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Micologia ClínicaInstituto de Química UNESP - Univ Estadual Paulista Departamento de Bioquímica e Tecnologia Química Unidade de Sintese Estrutura e Caracterizacao de Peptideos e ProteinasFaculdade de Ciências Farmacéuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Imunologia ClínicaFAPESP: 2015/03700-9Universidade Estadual Paulista (Unesp)Marcos, Caroline Maria [UNESP]de Oliveira, Haroldo Cesar [UNESP]da Silva, Julhiany de Fátima [UNESP]Assato, Patricia Akemi [UNESP]Yamazaki, Daniella Sayuri [UNESP]da Silva, Rosângela Aparecida Moraes [UNESP]Santos, Cláudia Tavares [UNESP]Santos-Filho, Norival Alves [UNESP]Portuondo, Deivys Leandro [UNESP]Mendes-Giannini, Maria José Soares [UNESP]Fusco-Almeida, Ana Marisa [UNESP]2018-12-11T17:07:39Z2018-12-11T17:07:39Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1093/femsyr/fow079FEMS Yeast Research, v. 16, n. 7, 2016.1567-13641567-1356http://hdl.handle.net/11449/17376910.1093/femsyr/fow0792-s2.0-849947179652-s2.0-84994717965.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFEMS Yeast Research1,3081,308info:eu-repo/semantics/openAccess2024-06-21T15:18:56Zoai:repositorio.unesp.br:11449/173769Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:15:51.779428Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interaction
title Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interaction
spellingShingle Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interaction
Marcos, Caroline Maria [UNESP]
EF-Tu
fibronectin
fungal-host interaction
moonlighting protein
Paracoccidioides brasiliensis
plasminogen
title_short Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interaction
title_full Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interaction
title_fullStr Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interaction
title_full_unstemmed Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interaction
title_sort Identification and characterisation of elongation factor Tu, a novel protein involved in Paracoccidioides brasiliensis-host interaction
author Marcos, Caroline Maria [UNESP]
author_facet Marcos, Caroline Maria [UNESP]
de Oliveira, Haroldo Cesar [UNESP]
da Silva, Julhiany de Fátima [UNESP]
Assato, Patricia Akemi [UNESP]
Yamazaki, Daniella Sayuri [UNESP]
da Silva, Rosângela Aparecida Moraes [UNESP]
Santos, Cláudia Tavares [UNESP]
Santos-Filho, Norival Alves [UNESP]
Portuondo, Deivys Leandro [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
Fusco-Almeida, Ana Marisa [UNESP]
author_role author
author2 de Oliveira, Haroldo Cesar [UNESP]
da Silva, Julhiany de Fátima [UNESP]
Assato, Patricia Akemi [UNESP]
Yamazaki, Daniella Sayuri [UNESP]
da Silva, Rosângela Aparecida Moraes [UNESP]
Santos, Cláudia Tavares [UNESP]
Santos-Filho, Norival Alves [UNESP]
Portuondo, Deivys Leandro [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
Fusco-Almeida, Ana Marisa [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Marcos, Caroline Maria [UNESP]
de Oliveira, Haroldo Cesar [UNESP]
da Silva, Julhiany de Fátima [UNESP]
Assato, Patricia Akemi [UNESP]
Yamazaki, Daniella Sayuri [UNESP]
da Silva, Rosângela Aparecida Moraes [UNESP]
Santos, Cláudia Tavares [UNESP]
Santos-Filho, Norival Alves [UNESP]
Portuondo, Deivys Leandro [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
Fusco-Almeida, Ana Marisa [UNESP]
dc.subject.por.fl_str_mv EF-Tu
fibronectin
fungal-host interaction
moonlighting protein
Paracoccidioides brasiliensis
plasminogen
topic EF-Tu
fibronectin
fungal-host interaction
moonlighting protein
Paracoccidioides brasiliensis
plasminogen
description Paracoccidioides spp., which are temperature-dependent dimorphic fungi, are responsible for the most prevalent human systemic mycosis in Latin America, the paracoccidioidomycosis. The aim of this study was to characterise the involvement of elongation factor Tu (EF-Tu) in Paracoccidioides brasiliensis-host interaction. Adhesive properties were examined using recombinant PbEF-Tu proteins and the respective polyclonal anti-rPbEF-Tu antibody. Immunogold analysis demonstrated the surface location of EF-Tu in P. brasiliensis. Moreover, PbEF-Tu was found to bind to fibronectin and plasminogen by enzyme-linked immunosorbent assay, and it was determined that the binding to plasminogen is at least partly dependent on lysine residues and ionic interactions. To verify the participation of EF-Tu in the interaction of P. brasiliensis with pneumocytes, we blocked the respective protein with an anti-rPbEF-Tu antibody and evaluated the consequences on the interaction index by flow cytometry. During the interaction, we observed a decrease of 2- and 3-fold at 8 and 24 h, respectively, suggesting the contribution of EF-Tu in fungal adhesion/invasion.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-01
2018-12-11T17:07:39Z
2018-12-11T17:07:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1093/femsyr/fow079
FEMS Yeast Research, v. 16, n. 7, 2016.
1567-1364
1567-1356
http://hdl.handle.net/11449/173769
10.1093/femsyr/fow079
2-s2.0-84994717965
2-s2.0-84994717965.pdf
url http://dx.doi.org/10.1093/femsyr/fow079
http://hdl.handle.net/11449/173769
identifier_str_mv FEMS Yeast Research, v. 16, n. 7, 2016.
1567-1364
1567-1356
10.1093/femsyr/fow079
2-s2.0-84994717965
2-s2.0-84994717965.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv FEMS Yeast Research
1,308
1,308
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128913340104704