Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/gmr18700 http://hdl.handle.net/11449/210052 |
Resumo: | Microsatellite markers, also known as single sequence repeats (SSRs), are highly polymorphic, fast evolving, and regarded as neutral markers. Due to these traits, they have been widely used in population studies. The development of SSRs for a given species opens possibilities for their application in population studies of other species that are phylogenetically related. We tested 16 primer pairs developed for Drosophila sturtevanti and the transferability test of them in 14 species of the saltans group of Drosophila. The optimal amplification conditions were established using the DNA of 15 D. sturtevanti males. Among the primers pairs developed, 13 have successful in the amplification for D. sturtevanti. And in the transferability test, the total percentage of transfer was nearly 50%. The species with the highest success rates of heterologous amplification were in the sturtevanti subgroup. Two microsatellite markers amplified in all the species, while one would not amplify for any of the saltans group species. These data demonstrate the usefulness of testing transferability of genetic markers, which may be used in studies of genetic diversity and population structure of D. sturtevanti and other species of the saltans group of Drosophila. |
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Repositório Institucional da UNESP |
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Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae)Genetic diversityGenomic libraryMolecular markersNeutral markersSSRMicrosatellite markers, also known as single sequence repeats (SSRs), are highly polymorphic, fast evolving, and regarded as neutral markers. Due to these traits, they have been widely used in population studies. The development of SSRs for a given species opens possibilities for their application in population studies of other species that are phylogenetically related. We tested 16 primer pairs developed for Drosophila sturtevanti and the transferability test of them in 14 species of the saltans group of Drosophila. The optimal amplification conditions were established using the DNA of 15 D. sturtevanti males. Among the primers pairs developed, 13 have successful in the amplification for D. sturtevanti. And in the transferability test, the total percentage of transfer was nearly 50%. The species with the highest success rates of heterologous amplification were in the sturtevanti subgroup. Two microsatellite markers amplified in all the species, while one would not amplify for any of the saltans group species. These data demonstrate the usefulness of testing transferability of genetic markers, which may be used in studies of genetic diversity and population structure of D. sturtevanti and other species of the saltans group of Drosophila.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP, BrazilUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP, BrazilFAPESP: 2014/14059-0FAPESP: 2015/17579-7FAPESP: 2017/05344-0Funpec-editoraUniversidade Estadual Paulista (Unesp)Roman, B. E. [UNESP]Trava, B. M. [UNESP]Madi-Ravazzi, L. [UNESP]2021-06-25T12:38:12Z2021-06-25T12:38:12Z2020-10-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7http://dx.doi.org/10.4238/gmr18700Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 19, n. 4, 7 p., 2020.http://hdl.handle.net/11449/21005210.4238/gmr18700WOS:000619558000001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Researchinfo:eu-repo/semantics/openAccess2021-10-23T20:11:08Zoai:repositorio.unesp.br:11449/210052Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:11:51.336457Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae) |
title |
Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae) |
spellingShingle |
Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae) Roman, B. E. [UNESP] Genetic diversity Genomic library Molecular markers Neutral markers SSR |
title_short |
Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae) |
title_full |
Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae) |
title_fullStr |
Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae) |
title_full_unstemmed |
Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae) |
title_sort |
Development and transferability of microsatellite markers for species of the saltans group of Drosophila (Diptera: Drosophilidae) |
author |
Roman, B. E. [UNESP] |
author_facet |
Roman, B. E. [UNESP] Trava, B. M. [UNESP] Madi-Ravazzi, L. [UNESP] |
author_role |
author |
author2 |
Trava, B. M. [UNESP] Madi-Ravazzi, L. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Roman, B. E. [UNESP] Trava, B. M. [UNESP] Madi-Ravazzi, L. [UNESP] |
dc.subject.por.fl_str_mv |
Genetic diversity Genomic library Molecular markers Neutral markers SSR |
topic |
Genetic diversity Genomic library Molecular markers Neutral markers SSR |
description |
Microsatellite markers, also known as single sequence repeats (SSRs), are highly polymorphic, fast evolving, and regarded as neutral markers. Due to these traits, they have been widely used in population studies. The development of SSRs for a given species opens possibilities for their application in population studies of other species that are phylogenetically related. We tested 16 primer pairs developed for Drosophila sturtevanti and the transferability test of them in 14 species of the saltans group of Drosophila. The optimal amplification conditions were established using the DNA of 15 D. sturtevanti males. Among the primers pairs developed, 13 have successful in the amplification for D. sturtevanti. And in the transferability test, the total percentage of transfer was nearly 50%. The species with the highest success rates of heterologous amplification were in the sturtevanti subgroup. Two microsatellite markers amplified in all the species, while one would not amplify for any of the saltans group species. These data demonstrate the usefulness of testing transferability of genetic markers, which may be used in studies of genetic diversity and population structure of D. sturtevanti and other species of the saltans group of Drosophila. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-30 2021-06-25T12:38:12Z 2021-06-25T12:38:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/gmr18700 Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 19, n. 4, 7 p., 2020. http://hdl.handle.net/11449/210052 10.4238/gmr18700 WOS:000619558000001 |
url |
http://dx.doi.org/10.4238/gmr18700 http://hdl.handle.net/11449/210052 |
identifier_str_mv |
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 19, n. 4, 7 p., 2020. 10.4238/gmr18700 WOS:000619558000001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics And Molecular Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 |
dc.publisher.none.fl_str_mv |
Funpec-editora |
publisher.none.fl_str_mv |
Funpec-editora |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129498528350208 |