Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/md21030197 http://hdl.handle.net/11449/247092 |
Resumo: | Sea anemones are sessile invertebrates of the phylum Cnidaria and their survival and evolutive success are highly related to the ability to produce and quickly inoculate venom, with the presence of potent toxins. In this study, a multi-omics approach was applied to characterize the protein composition of the tentacles and mucus of Bunodosoma caissarum, a species of sea anemone from the Brazilian coast. The tentacles transcriptome resulted in 23,444 annotated genes, of which 1% showed similarity with toxins or proteins related to toxin activity. In the proteome analysis, 430 polypeptides were consistently identified: 316 of them were more abundant in the tentacles while 114 were enriched in the mucus. Tentacle proteins were mostly enzymes, followed by DNA- and RNA-associated proteins, while in the mucus most proteins were toxins. In addition, peptidomics allowed the identification of large and small fragments of mature toxins, neuropeptides, and intracellular peptides. In conclusion, integrated omics identified previously unknown or uncharacterized genes in addition to 23 toxin-like proteins of therapeutic potential, improving the understanding of tentacle and mucus composition of sea anemones. |
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Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarumintracellular peptidesneuropeptidesomicssea anemonetoxinsSea anemones are sessile invertebrates of the phylum Cnidaria and their survival and evolutive success are highly related to the ability to produce and quickly inoculate venom, with the presence of potent toxins. In this study, a multi-omics approach was applied to characterize the protein composition of the tentacles and mucus of Bunodosoma caissarum, a species of sea anemone from the Brazilian coast. The tentacles transcriptome resulted in 23,444 annotated genes, of which 1% showed similarity with toxins or proteins related to toxin activity. In the proteome analysis, 430 polypeptides were consistently identified: 316 of them were more abundant in the tentacles while 114 were enriched in the mucus. Tentacle proteins were mostly enzymes, followed by DNA- and RNA-associated proteins, while in the mucus most proteins were toxins. In addition, peptidomics allowed the identification of large and small fragments of mature toxins, neuropeptides, and intracellular peptides. In conclusion, integrated omics identified previously unknown or uncharacterized genes in addition to 23 toxin-like proteins of therapeutic potential, improving the understanding of tentacle and mucus composition of sea anemones.Department of Biological and Environmental Sciences Bioscience Institute Sao Paulo State University (UNESP), SPBiodiversity of Coastal Environments Postgraduate Program Department of Biological and Environmental Sciences Bioscience Institute Sao Paulo State University (UNESP), SPNGS Soluções Genômicas, SPDepartment of Biological and Environmental Sciences Bioscience Institute Sao Paulo State University (UNESP), SPBiodiversity of Coastal Environments Postgraduate Program Department of Biological and Environmental Sciences Bioscience Institute Sao Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)NGS Soluções GenômicasMazzi Esquinca, Maria Eduarda [UNESP]Correa, Claudia Neves [UNESP]Marques de Barros, Gabriel [UNESP]Montenegro, HorácioMantovani de Castro, Leandro [UNESP]2023-07-29T13:06:02Z2023-07-29T13:06:02Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/md21030197Marine Drugs, v. 21, n. 3, 2023.1660-3397http://hdl.handle.net/11449/24709210.3390/md210301972-s2.0-85151398535Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMarine Drugsinfo:eu-repo/semantics/openAccess2023-07-29T13:06:02Zoai:repositorio.unesp.br:11449/247092Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:50:47.030749Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum |
title |
Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum |
spellingShingle |
Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum Mazzi Esquinca, Maria Eduarda [UNESP] intracellular peptides neuropeptides omics sea anemone toxins |
title_short |
Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum |
title_full |
Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum |
title_fullStr |
Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum |
title_full_unstemmed |
Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum |
title_sort |
Multiomic Approach for Bioprospection: Investigation of Toxins and Peptides of Brazilian Sea Anemone Bunodosoma caissarum |
author |
Mazzi Esquinca, Maria Eduarda [UNESP] |
author_facet |
Mazzi Esquinca, Maria Eduarda [UNESP] Correa, Claudia Neves [UNESP] Marques de Barros, Gabriel [UNESP] Montenegro, Horácio Mantovani de Castro, Leandro [UNESP] |
author_role |
author |
author2 |
Correa, Claudia Neves [UNESP] Marques de Barros, Gabriel [UNESP] Montenegro, Horácio Mantovani de Castro, Leandro [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) NGS Soluções Genômicas |
dc.contributor.author.fl_str_mv |
Mazzi Esquinca, Maria Eduarda [UNESP] Correa, Claudia Neves [UNESP] Marques de Barros, Gabriel [UNESP] Montenegro, Horácio Mantovani de Castro, Leandro [UNESP] |
dc.subject.por.fl_str_mv |
intracellular peptides neuropeptides omics sea anemone toxins |
topic |
intracellular peptides neuropeptides omics sea anemone toxins |
description |
Sea anemones are sessile invertebrates of the phylum Cnidaria and their survival and evolutive success are highly related to the ability to produce and quickly inoculate venom, with the presence of potent toxins. In this study, a multi-omics approach was applied to characterize the protein composition of the tentacles and mucus of Bunodosoma caissarum, a species of sea anemone from the Brazilian coast. The tentacles transcriptome resulted in 23,444 annotated genes, of which 1% showed similarity with toxins or proteins related to toxin activity. In the proteome analysis, 430 polypeptides were consistently identified: 316 of them were more abundant in the tentacles while 114 were enriched in the mucus. Tentacle proteins were mostly enzymes, followed by DNA- and RNA-associated proteins, while in the mucus most proteins were toxins. In addition, peptidomics allowed the identification of large and small fragments of mature toxins, neuropeptides, and intracellular peptides. In conclusion, integrated omics identified previously unknown or uncharacterized genes in addition to 23 toxin-like proteins of therapeutic potential, improving the understanding of tentacle and mucus composition of sea anemones. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:06:02Z 2023-07-29T13:06:02Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/md21030197 Marine Drugs, v. 21, n. 3, 2023. 1660-3397 http://hdl.handle.net/11449/247092 10.3390/md21030197 2-s2.0-85151398535 |
url |
http://dx.doi.org/10.3390/md21030197 http://hdl.handle.net/11449/247092 |
identifier_str_mv |
Marine Drugs, v. 21, n. 3, 2023. 1660-3397 10.3390/md21030197 2-s2.0-85151398535 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Marine Drugs |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128989501325312 |