Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440201701393 http://hdl.handle.net/11449/179420 |
Resumo: | Recently, the non-albicans Candida species have become recognized as an important source of infection and oral colonization by association of different species in a large number of immunosuppressed patients. The objective of this study was to evaluate the interactions between C. krusei and C. glabrata in biofilms formed in vitro and their ability to colonize the oral cavity of mouse model. Monospecies and mixed biofilms were developed of each strain, on 96-well microtiter plates for 48 h. These biofilms were analyzed by counting colony-forming units (CFU/mL) and by determining cell viability, using the XTT hydroxide colorimetric assay. For the in vivo study, twenty-four mice received topical applications of monospecie or mixed suspensions of each strain. After 48 h, yeasts were recovered from the mice and quantified by CFU/mL count. In the biofilm assays, the results for the CFU/mL count and the XTT assay showed that the two species studied were capable of forming high levels of in vitro monospecie biofilm. In mixed biofilm, the CFU of C. krusei increased (p=0.0001) and C. glabrata decreased (p=0.0001). The metabolic activity observed in XTT assay of mixed biofilm was significantly reduced compared with a single C. glabrata biofilm (p=0.0001). Agreeing with CFU in vitro count, C. glabrata CFU/mL values recovered from oral cavity of mice were statistically higher in the group with single infection (p=0.0001) than the group with mixed infection. We concluded that C. krusei inhibits C. glabrata and takes advantage to colonize the oral cavity and to form biofilms. |
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Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studiesBiofilmsCandida glabrataCandida kruseiExperimental candidiasisMurine modelRecently, the non-albicans Candida species have become recognized as an important source of infection and oral colonization by association of different species in a large number of immunosuppressed patients. The objective of this study was to evaluate the interactions between C. krusei and C. glabrata in biofilms formed in vitro and their ability to colonize the oral cavity of mouse model. Monospecies and mixed biofilms were developed of each strain, on 96-well microtiter plates for 48 h. These biofilms were analyzed by counting colony-forming units (CFU/mL) and by determining cell viability, using the XTT hydroxide colorimetric assay. For the in vivo study, twenty-four mice received topical applications of monospecie or mixed suspensions of each strain. After 48 h, yeasts were recovered from the mice and quantified by CFU/mL count. In the biofilm assays, the results for the CFU/mL count and the XTT assay showed that the two species studied were capable of forming high levels of in vitro monospecie biofilm. In mixed biofilm, the CFU of C. krusei increased (p=0.0001) and C. glabrata decreased (p=0.0001). The metabolic activity observed in XTT assay of mixed biofilm was significantly reduced compared with a single C. glabrata biofilm (p=0.0001). Agreeing with CFU in vitro count, C. glabrata CFU/mL values recovered from oral cavity of mice were statistically higher in the group with single infection (p=0.0001) than the group with mixed infection. We concluded that C. krusei inhibits C. glabrata and takes advantage to colonize the oral cavity and to form biofilms.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP-Universidade Estadual PaulistaDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP-Universidade Estadual PaulistaFAPESP: #2012/02184-9FAPESP: #2013/25181-8Universidade Estadual Paulista (Unesp)Rossoni, Rodnei Dennis [UNESP]de Barros, Patrícia Pimentel [UNESP]Freire, Fernanda [UNESP]dos Santos, Jéssica Diane [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]Junqueira, Juliana Campos [UNESP]2018-12-11T17:35:07Z2018-12-11T17:35:07Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article669-674application/pdfhttp://dx.doi.org/10.1590/0103-6440201701393Brazilian Dental Journal, v. 28, n. 6, p. 669-674, 2017.1806-47600103-6440http://hdl.handle.net/11449/17942010.1590/0103-6440201701393S0103-644020170006006692-s2.0-85037371247S0103-64402017000600669.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2023-11-06T06:12:56Zoai:repositorio.unesp.br:11449/179420Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-06T06:12:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies |
title |
Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies |
spellingShingle |
Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies Rossoni, Rodnei Dennis [UNESP] Biofilms Candida glabrata Candida krusei Experimental candidiasis Murine model |
title_short |
Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies |
title_full |
Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies |
title_fullStr |
Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies |
title_full_unstemmed |
Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies |
title_sort |
Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies |
author |
Rossoni, Rodnei Dennis [UNESP] |
author_facet |
Rossoni, Rodnei Dennis [UNESP] de Barros, Patrícia Pimentel [UNESP] Freire, Fernanda [UNESP] dos Santos, Jéssica Diane [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos [UNESP] |
author_role |
author |
author2 |
de Barros, Patrícia Pimentel [UNESP] Freire, Fernanda [UNESP] dos Santos, Jéssica Diane [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rossoni, Rodnei Dennis [UNESP] de Barros, Patrícia Pimentel [UNESP] Freire, Fernanda [UNESP] dos Santos, Jéssica Diane [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms Candida glabrata Candida krusei Experimental candidiasis Murine model |
topic |
Biofilms Candida glabrata Candida krusei Experimental candidiasis Murine model |
description |
Recently, the non-albicans Candida species have become recognized as an important source of infection and oral colonization by association of different species in a large number of immunosuppressed patients. The objective of this study was to evaluate the interactions between C. krusei and C. glabrata in biofilms formed in vitro and their ability to colonize the oral cavity of mouse model. Monospecies and mixed biofilms were developed of each strain, on 96-well microtiter plates for 48 h. These biofilms were analyzed by counting colony-forming units (CFU/mL) and by determining cell viability, using the XTT hydroxide colorimetric assay. For the in vivo study, twenty-four mice received topical applications of monospecie or mixed suspensions of each strain. After 48 h, yeasts were recovered from the mice and quantified by CFU/mL count. In the biofilm assays, the results for the CFU/mL count and the XTT assay showed that the two species studied were capable of forming high levels of in vitro monospecie biofilm. In mixed biofilm, the CFU of C. krusei increased (p=0.0001) and C. glabrata decreased (p=0.0001). The metabolic activity observed in XTT assay of mixed biofilm was significantly reduced compared with a single C. glabrata biofilm (p=0.0001). Agreeing with CFU in vitro count, C. glabrata CFU/mL values recovered from oral cavity of mice were statistically higher in the group with single infection (p=0.0001) than the group with mixed infection. We concluded that C. krusei inhibits C. glabrata and takes advantage to colonize the oral cavity and to form biofilms. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-12-11T17:35:07Z 2018-12-11T17:35:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201701393 Brazilian Dental Journal, v. 28, n. 6, p. 669-674, 2017. 1806-4760 0103-6440 http://hdl.handle.net/11449/179420 10.1590/0103-6440201701393 S0103-64402017000600669 2-s2.0-85037371247 S0103-64402017000600669.pdf |
url |
http://dx.doi.org/10.1590/0103-6440201701393 http://hdl.handle.net/11449/179420 |
identifier_str_mv |
Brazilian Dental Journal, v. 28, n. 6, p. 669-674, 2017. 1806-4760 0103-6440 10.1590/0103-6440201701393 S0103-64402017000600669 2-s2.0-85037371247 S0103-64402017000600669.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal 0,476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
669-674 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964841928032256 |