Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability

Detalhes bibliográficos
Autor(a) principal: Ferrasi, Adriana Camargo [UNESP]
Data de Publicação: 2010
Outros Autores: Pinheiro, Nidia Alice [UNESP], Barem Rabenhorst, Silvia Helena, Caballero, Otavia Luisa, Rodrigues, Maria Aparecida Marchesan [UNESP], de Carvalho, Fabricio, Leite, Celso Vieira de Souza [UNESP], Pitombeira Ferreira, Marcia Valeria, Pessoa Barros, Marcos Aurelio, Pardini, Maria Ines de Moura Campos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3748/wjg.v16.i3.312
http://hdl.handle.net/11449/11117
Resumo: AIM: To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA(+) and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H, pylori and genotyping were performed by PCR, using specific primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the chi(2) or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA(+) in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H, pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. (C) 2010 Baishideng. All rights reserved.
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spelling Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instabilityGastric cancerMethylationMicrosatellite instabilityHelicobacter pyloriEpstein Barr virusAIM: To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA(+) and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H, pylori and genotyping were performed by PCR, using specific primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the chi(2) or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA(+) in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H, pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. (C) 2010 Baishideng. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP São Paulo State Univ, Ctr Blood Transfus, Botucatu Med Sch, BR-18618970 Botucatu, SP, BrazilUniversidade Federal do Ceará (UFC), Dept Pathol, BR-60430160 Fortaleza, Ceara, BrazilUNESP São Paulo State Univ, Dept Pathol, Sch Med, BR-18618970 Botucatu, SP, BrazilLudwig Inst Canc Res, New York, NY 10065 USALudwig Inst Canc Res, BR-01323903 São Paulo, BrazilUNESP São Paulo State Univ, Dept Surg Gastroenterol, Sch Med, BR-18618970 Botucatu, SP, BrazilUNESP São Paulo State Univ, Ctr Blood Transfus, Botucatu Med Sch, BR-18618970 Botucatu, SP, BrazilUNESP São Paulo State Univ, Dept Pathol, Sch Med, BR-18618970 Botucatu, SP, BrazilUNESP São Paulo State Univ, Dept Surg Gastroenterol, Sch Med, BR-18618970 Botucatu, SP, BrazilW J G PressUniversidade Estadual Paulista (Unesp)Universidade Federal do Ceará (UFC)Ludwig Inst Canc ResFerrasi, Adriana Camargo [UNESP]Pinheiro, Nidia Alice [UNESP]Barem Rabenhorst, Silvia HelenaCaballero, Otavia LuisaRodrigues, Maria Aparecida Marchesan [UNESP]de Carvalho, FabricioLeite, Celso Vieira de Souza [UNESP]Pitombeira Ferreira, Marcia ValeriaPessoa Barros, Marcos AurelioPardini, Maria Ines de Moura Campos [UNESP]2014-05-20T13:32:36Z2014-05-20T13:32:36Z2010-01-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article312-319http://dx.doi.org/10.3748/wjg.v16.i3.312World Journal of Gastroenterology. Beijing: W J G Press, v. 16, n. 3, p. 312-319, 2010.1007-9327http://hdl.handle.net/11449/1111710.3748/wjg.v16.i3.312WOS:0002738847000063191894452135777358789508522622446195883345820840000-0001-9200-5391Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWorld Journal of Gastroenterology3.3001,409info:eu-repo/semantics/openAccess2024-09-03T13:18:44Zoai:repositorio.unesp.br:11449/11117Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
title Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
spellingShingle Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
Ferrasi, Adriana Camargo [UNESP]
Gastric cancer
Methylation
Microsatellite instability
Helicobacter pylori
Epstein Barr virus
title_short Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
title_full Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
title_fullStr Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
title_full_unstemmed Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
title_sort Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
author Ferrasi, Adriana Camargo [UNESP]
author_facet Ferrasi, Adriana Camargo [UNESP]
Pinheiro, Nidia Alice [UNESP]
Barem Rabenhorst, Silvia Helena
Caballero, Otavia Luisa
Rodrigues, Maria Aparecida Marchesan [UNESP]
de Carvalho, Fabricio
Leite, Celso Vieira de Souza [UNESP]
Pitombeira Ferreira, Marcia Valeria
Pessoa Barros, Marcos Aurelio
Pardini, Maria Ines de Moura Campos [UNESP]
author_role author
author2 Pinheiro, Nidia Alice [UNESP]
Barem Rabenhorst, Silvia Helena
Caballero, Otavia Luisa
Rodrigues, Maria Aparecida Marchesan [UNESP]
de Carvalho, Fabricio
Leite, Celso Vieira de Souza [UNESP]
Pitombeira Ferreira, Marcia Valeria
Pessoa Barros, Marcos Aurelio
Pardini, Maria Ines de Moura Campos [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal do Ceará (UFC)
Ludwig Inst Canc Res
dc.contributor.author.fl_str_mv Ferrasi, Adriana Camargo [UNESP]
Pinheiro, Nidia Alice [UNESP]
Barem Rabenhorst, Silvia Helena
Caballero, Otavia Luisa
Rodrigues, Maria Aparecida Marchesan [UNESP]
de Carvalho, Fabricio
Leite, Celso Vieira de Souza [UNESP]
Pitombeira Ferreira, Marcia Valeria
Pessoa Barros, Marcos Aurelio
Pardini, Maria Ines de Moura Campos [UNESP]
dc.subject.por.fl_str_mv Gastric cancer
Methylation
Microsatellite instability
Helicobacter pylori
Epstein Barr virus
topic Gastric cancer
Methylation
Microsatellite instability
Helicobacter pylori
Epstein Barr virus
description AIM: To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA(+) and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H, pylori and genotyping were performed by PCR, using specific primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the chi(2) or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA(+) in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H, pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. (C) 2010 Baishideng. All rights reserved.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-21
2014-05-20T13:32:36Z
2014-05-20T13:32:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3748/wjg.v16.i3.312
World Journal of Gastroenterology. Beijing: W J G Press, v. 16, n. 3, p. 312-319, 2010.
1007-9327
http://hdl.handle.net/11449/11117
10.3748/wjg.v16.i3.312
WOS:000273884700006
3191894452135777
3587895085226224
4619588334582084
0000-0001-9200-5391
url http://dx.doi.org/10.3748/wjg.v16.i3.312
http://hdl.handle.net/11449/11117
identifier_str_mv World Journal of Gastroenterology. Beijing: W J G Press, v. 16, n. 3, p. 312-319, 2010.
1007-9327
10.3748/wjg.v16.i3.312
WOS:000273884700006
3191894452135777
3587895085226224
4619588334582084
0000-0001-9200-5391
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv World Journal of Gastroenterology
3.300
1,409
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 312-319
dc.publisher.none.fl_str_mv W J G Press
publisher.none.fl_str_mv W J G Press
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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