Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3748/wjg.v16.i3.312 http://hdl.handle.net/11449/11117 |
Resumo: | AIM: To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA(+) and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H, pylori and genotyping were performed by PCR, using specific primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the chi(2) or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA(+) in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H, pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. (C) 2010 Baishideng. All rights reserved. |
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Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instabilityGastric cancerMethylationMicrosatellite instabilityHelicobacter pyloriEpstein Barr virusAIM: To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA(+) and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H, pylori and genotyping were performed by PCR, using specific primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the chi(2) or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA(+) in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H, pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. (C) 2010 Baishideng. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP São Paulo State Univ, Ctr Blood Transfus, Botucatu Med Sch, BR-18618970 Botucatu, SP, BrazilUniversidade Federal do Ceará (UFC), Dept Pathol, BR-60430160 Fortaleza, Ceara, BrazilUNESP São Paulo State Univ, Dept Pathol, Sch Med, BR-18618970 Botucatu, SP, BrazilLudwig Inst Canc Res, New York, NY 10065 USALudwig Inst Canc Res, BR-01323903 São Paulo, BrazilUNESP São Paulo State Univ, Dept Surg Gastroenterol, Sch Med, BR-18618970 Botucatu, SP, BrazilUNESP São Paulo State Univ, Ctr Blood Transfus, Botucatu Med Sch, BR-18618970 Botucatu, SP, BrazilUNESP São Paulo State Univ, Dept Pathol, Sch Med, BR-18618970 Botucatu, SP, BrazilUNESP São Paulo State Univ, Dept Surg Gastroenterol, Sch Med, BR-18618970 Botucatu, SP, BrazilW J G PressUniversidade Estadual Paulista (Unesp)Universidade Federal do Ceará (UFC)Ludwig Inst Canc ResFerrasi, Adriana Camargo [UNESP]Pinheiro, Nidia Alice [UNESP]Barem Rabenhorst, Silvia HelenaCaballero, Otavia LuisaRodrigues, Maria Aparecida Marchesan [UNESP]de Carvalho, FabricioLeite, Celso Vieira de Souza [UNESP]Pitombeira Ferreira, Marcia ValeriaPessoa Barros, Marcos AurelioPardini, Maria Ines de Moura Campos [UNESP]2014-05-20T13:32:36Z2014-05-20T13:32:36Z2010-01-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article312-319http://dx.doi.org/10.3748/wjg.v16.i3.312World Journal of Gastroenterology. Beijing: W J G Press, v. 16, n. 3, p. 312-319, 2010.1007-9327http://hdl.handle.net/11449/1111710.3748/wjg.v16.i3.312WOS:0002738847000063191894452135777358789508522622446195883345820840000-0001-9200-5391Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWorld Journal of Gastroenterology3.3001,409info:eu-repo/semantics/openAccess2024-09-03T13:18:44Zoai:repositorio.unesp.br:11449/11117Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability |
title |
Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability |
spellingShingle |
Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability Ferrasi, Adriana Camargo [UNESP] Gastric cancer Methylation Microsatellite instability Helicobacter pylori Epstein Barr virus |
title_short |
Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability |
title_full |
Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability |
title_fullStr |
Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability |
title_full_unstemmed |
Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability |
title_sort |
Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability |
author |
Ferrasi, Adriana Camargo [UNESP] |
author_facet |
Ferrasi, Adriana Camargo [UNESP] Pinheiro, Nidia Alice [UNESP] Barem Rabenhorst, Silvia Helena Caballero, Otavia Luisa Rodrigues, Maria Aparecida Marchesan [UNESP] de Carvalho, Fabricio Leite, Celso Vieira de Souza [UNESP] Pitombeira Ferreira, Marcia Valeria Pessoa Barros, Marcos Aurelio Pardini, Maria Ines de Moura Campos [UNESP] |
author_role |
author |
author2 |
Pinheiro, Nidia Alice [UNESP] Barem Rabenhorst, Silvia Helena Caballero, Otavia Luisa Rodrigues, Maria Aparecida Marchesan [UNESP] de Carvalho, Fabricio Leite, Celso Vieira de Souza [UNESP] Pitombeira Ferreira, Marcia Valeria Pessoa Barros, Marcos Aurelio Pardini, Maria Ines de Moura Campos [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do Ceará (UFC) Ludwig Inst Canc Res |
dc.contributor.author.fl_str_mv |
Ferrasi, Adriana Camargo [UNESP] Pinheiro, Nidia Alice [UNESP] Barem Rabenhorst, Silvia Helena Caballero, Otavia Luisa Rodrigues, Maria Aparecida Marchesan [UNESP] de Carvalho, Fabricio Leite, Celso Vieira de Souza [UNESP] Pitombeira Ferreira, Marcia Valeria Pessoa Barros, Marcos Aurelio Pardini, Maria Ines de Moura Campos [UNESP] |
dc.subject.por.fl_str_mv |
Gastric cancer Methylation Microsatellite instability Helicobacter pylori Epstein Barr virus |
topic |
Gastric cancer Methylation Microsatellite instability Helicobacter pylori Epstein Barr virus |
description |
AIM: To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA(+) and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H, pylori and genotyping were performed by PCR, using specific primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the chi(2) or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA(+) in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H, pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. (C) 2010 Baishideng. All rights reserved. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-21 2014-05-20T13:32:36Z 2014-05-20T13:32:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3748/wjg.v16.i3.312 World Journal of Gastroenterology. Beijing: W J G Press, v. 16, n. 3, p. 312-319, 2010. 1007-9327 http://hdl.handle.net/11449/11117 10.3748/wjg.v16.i3.312 WOS:000273884700006 3191894452135777 3587895085226224 4619588334582084 0000-0001-9200-5391 |
url |
http://dx.doi.org/10.3748/wjg.v16.i3.312 http://hdl.handle.net/11449/11117 |
identifier_str_mv |
World Journal of Gastroenterology. Beijing: W J G Press, v. 16, n. 3, p. 312-319, 2010. 1007-9327 10.3748/wjg.v16.i3.312 WOS:000273884700006 3191894452135777 3587895085226224 4619588334582084 0000-0001-9200-5391 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
World Journal of Gastroenterology 3.300 1,409 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
312-319 |
dc.publisher.none.fl_str_mv |
W J G Press |
publisher.none.fl_str_mv |
W J G Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021426356289536 |