Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-230X-14-179 http://repositorio.unifesp.br/handle/11600/38330 |
Resumo: | Background: Helicobacter pylori (HP) and Epstein-Barr virus (EBV) have been associated with cancer development. We evaluated the prevalence of HP, HP CagA(+) and EBV infection in gastric cancer (GC) samples from adults and in gastric tissues from patients who underwent upper endoscopy (UE).Methods: Samples from UE and GC were collected to investigate the presence of HP infection and the HP virulence factor CagA by a urease test and PCR. the presence of EBV was detected by Eber-1 in situ hybridization.Results: in UE, 85.5% of juvenile patients showed some degree of gastritis (45.3% of patients with mild gastritis and 54.7% with moderate/severe gastritis) and patients with mild gastritis were younger than patients with moderate/severe gastritis. Among adults, 48.7% presented mild gastritis and 51.3% moderate/severe gastritis. HP infection was detected in 0% of normal mucosa, 58.5% of juvenile gastritis patients, 69.2% of adult gastritis patients and 88% of GC patients. in these same groups, HP CagA(+) was detected in 0%, 37.7%, 61.5% and 67.2% of tissue samples, respectively. in juvenile patients, HP infection was more common in those with gastritis than in normal samples (p = 0.004). the patients with either HP or HP CagA(+) were older than patients without these pathogens (p < 0.05). in juvenile patients, HP infection was more frequent in cases of moderate/severe gastritis than in cases of mild gastritis (p = 0.026). Moreover, in patients with GC, HP infection was more frequent in males than in females (p = 0.023). GC patients with HP CagA(+) were older than patients with HP CagA-(p = 0.027). HP CagA(+) was more common in intestinal-type than diffuse-type GC (p = 0.012). HP CagA(+) was also associated with lymph-node (p = 0.024) and distal (p = 0.005) metastasis. No association between EBV infection and HP infection or any clinicopathological variable was detected.Conclusions: Our results suggest that HP is involved in the pathophysiology of severe gastric lesions and in the development of GC, particularly when CagA(+) is present. EBV was not the primary pathogenic factor in our samples. |
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Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern BrazilHelicobacter pyloriEpstein-Barr virusGastritisGastric cancerBackground: Helicobacter pylori (HP) and Epstein-Barr virus (EBV) have been associated with cancer development. We evaluated the prevalence of HP, HP CagA(+) and EBV infection in gastric cancer (GC) samples from adults and in gastric tissues from patients who underwent upper endoscopy (UE).Methods: Samples from UE and GC were collected to investigate the presence of HP infection and the HP virulence factor CagA by a urease test and PCR. the presence of EBV was detected by Eber-1 in situ hybridization.Results: in UE, 85.5% of juvenile patients showed some degree of gastritis (45.3% of patients with mild gastritis and 54.7% with moderate/severe gastritis) and patients with mild gastritis were younger than patients with moderate/severe gastritis. Among adults, 48.7% presented mild gastritis and 51.3% moderate/severe gastritis. HP infection was detected in 0% of normal mucosa, 58.5% of juvenile gastritis patients, 69.2% of adult gastritis patients and 88% of GC patients. in these same groups, HP CagA(+) was detected in 0%, 37.7%, 61.5% and 67.2% of tissue samples, respectively. in juvenile patients, HP infection was more common in those with gastritis than in normal samples (p = 0.004). the patients with either HP or HP CagA(+) were older than patients without these pathogens (p < 0.05). in juvenile patients, HP infection was more frequent in cases of moderate/severe gastritis than in cases of mild gastritis (p = 0.026). Moreover, in patients with GC, HP infection was more frequent in males than in females (p = 0.023). GC patients with HP CagA(+) were older than patients with HP CagA-(p = 0.027). HP CagA(+) was more common in intestinal-type than diffuse-type GC (p = 0.012). HP CagA(+) was also associated with lymph-node (p = 0.024) and distal (p = 0.005) metastasis. No association between EBV infection and HP infection or any clinicopathological variable was detected.Conclusions: Our results suggest that HP is involved in the pathophysiology of severe gastric lesions and in the development of GC, particularly when CagA(+) is present. EBV was not the primary pathogenic factor in our samples.Fed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66075110 Belem, PA, BrazilFed Univ Para, Inst Ciencias Saude, BR-66075110 Belem, PA, BrazilCtr Univ Para, Belem, PA, BrazilUniversidade Federal de São Paulo, Dept Ortopedia & Traumatol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, São Paulo, BrazilFed Univ Para, BR-66075110 Belem, PA, BrazilUniv Fed Ceara, Fac Odontol, Dept Oral Pathol, Fortaleza, CE, BrazilUniversidade Federal de São Paulo, Dept Ortopedia & Traumatol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Biomed Central LtdFed Univ ParaCtr Univ ParaUniversidade Federal de São Paulo (UNIFESP)Univ Fed CearaSouza, Carolina Rosal Teixeira deOliveira, Katia Soares deFerraz, Jefferson Jose SodreLeal, Mariana Ferreira [UNIFESP]Calcagno, Danielle Queiroz [UNIFESP]Seabra, Aline DamascenoKhayat, Andre SalimMontenegro, Raquel CarvalhoAlves, Ana Paula Negreiros NunesAssumpcao, Paulo PimentelSmith, Marilia de Arruda Cardoso [UNIFESP]Burbano, Rommel Rodriguez2016-01-24T14:38:00Z2016-01-24T14:38:00Z2014-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9application/pdfhttp://dx.doi.org/10.1186/1471-230X-14-179Bmc Gastroenterology. London: Biomed Central Ltd, v. 14, 9 p., 2014.10.1186/1471-230X-14-179WOS000346673400001.pdf1471-230Xhttp://repositorio.unifesp.br/handle/11600/38330WOS:000346673400001engBmc Gastroenterologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T05:46:10Zoai:repositorio.unifesp.br/:11600/38330Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T05:46:10Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil |
title |
Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil |
spellingShingle |
Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil Souza, Carolina Rosal Teixeira de Helicobacter pylori Epstein-Barr virus Gastritis Gastric cancer |
title_short |
Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil |
title_full |
Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil |
title_fullStr |
Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil |
title_full_unstemmed |
Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil |
title_sort |
Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil |
author |
Souza, Carolina Rosal Teixeira de |
author_facet |
Souza, Carolina Rosal Teixeira de Oliveira, Katia Soares de Ferraz, Jefferson Jose Sodre Leal, Mariana Ferreira [UNIFESP] Calcagno, Danielle Queiroz [UNIFESP] Seabra, Aline Damasceno Khayat, Andre Salim Montenegro, Raquel Carvalho Alves, Ana Paula Negreiros Nunes Assumpcao, Paulo Pimentel Smith, Marilia de Arruda Cardoso [UNIFESP] Burbano, Rommel Rodriguez |
author_role |
author |
author2 |
Oliveira, Katia Soares de Ferraz, Jefferson Jose Sodre Leal, Mariana Ferreira [UNIFESP] Calcagno, Danielle Queiroz [UNIFESP] Seabra, Aline Damasceno Khayat, Andre Salim Montenegro, Raquel Carvalho Alves, Ana Paula Negreiros Nunes Assumpcao, Paulo Pimentel Smith, Marilia de Arruda Cardoso [UNIFESP] Burbano, Rommel Rodriguez |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Fed Univ Para Ctr Univ Para Universidade Federal de São Paulo (UNIFESP) Univ Fed Ceara |
dc.contributor.author.fl_str_mv |
Souza, Carolina Rosal Teixeira de Oliveira, Katia Soares de Ferraz, Jefferson Jose Sodre Leal, Mariana Ferreira [UNIFESP] Calcagno, Danielle Queiroz [UNIFESP] Seabra, Aline Damasceno Khayat, Andre Salim Montenegro, Raquel Carvalho Alves, Ana Paula Negreiros Nunes Assumpcao, Paulo Pimentel Smith, Marilia de Arruda Cardoso [UNIFESP] Burbano, Rommel Rodriguez |
dc.subject.por.fl_str_mv |
Helicobacter pylori Epstein-Barr virus Gastritis Gastric cancer |
topic |
Helicobacter pylori Epstein-Barr virus Gastritis Gastric cancer |
description |
Background: Helicobacter pylori (HP) and Epstein-Barr virus (EBV) have been associated with cancer development. We evaluated the prevalence of HP, HP CagA(+) and EBV infection in gastric cancer (GC) samples from adults and in gastric tissues from patients who underwent upper endoscopy (UE).Methods: Samples from UE and GC were collected to investigate the presence of HP infection and the HP virulence factor CagA by a urease test and PCR. the presence of EBV was detected by Eber-1 in situ hybridization.Results: in UE, 85.5% of juvenile patients showed some degree of gastritis (45.3% of patients with mild gastritis and 54.7% with moderate/severe gastritis) and patients with mild gastritis were younger than patients with moderate/severe gastritis. Among adults, 48.7% presented mild gastritis and 51.3% moderate/severe gastritis. HP infection was detected in 0% of normal mucosa, 58.5% of juvenile gastritis patients, 69.2% of adult gastritis patients and 88% of GC patients. in these same groups, HP CagA(+) was detected in 0%, 37.7%, 61.5% and 67.2% of tissue samples, respectively. in juvenile patients, HP infection was more common in those with gastritis than in normal samples (p = 0.004). the patients with either HP or HP CagA(+) were older than patients without these pathogens (p < 0.05). in juvenile patients, HP infection was more frequent in cases of moderate/severe gastritis than in cases of mild gastritis (p = 0.026). Moreover, in patients with GC, HP infection was more frequent in males than in females (p = 0.023). GC patients with HP CagA(+) were older than patients with HP CagA-(p = 0.027). HP CagA(+) was more common in intestinal-type than diffuse-type GC (p = 0.012). HP CagA(+) was also associated with lymph-node (p = 0.024) and distal (p = 0.005) metastasis. No association between EBV infection and HP infection or any clinicopathological variable was detected.Conclusions: Our results suggest that HP is involved in the pathophysiology of severe gastric lesions and in the development of GC, particularly when CagA(+) is present. EBV was not the primary pathogenic factor in our samples. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-15 2016-01-24T14:38:00Z 2016-01-24T14:38:00Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-230X-14-179 Bmc Gastroenterology. London: Biomed Central Ltd, v. 14, 9 p., 2014. 10.1186/1471-230X-14-179 WOS000346673400001.pdf 1471-230X http://repositorio.unifesp.br/handle/11600/38330 WOS:000346673400001 |
url |
http://dx.doi.org/10.1186/1471-230X-14-179 http://repositorio.unifesp.br/handle/11600/38330 |
identifier_str_mv |
Bmc Gastroenterology. London: Biomed Central Ltd, v. 14, 9 p., 2014. 10.1186/1471-230X-14-179 WOS000346673400001.pdf 1471-230X WOS:000346673400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Gastroenterology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268463234940928 |