Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil

Detalhes bibliográficos
Autor(a) principal: Souza, Carolina Rosal Teixeira de
Data de Publicação: 2014
Outros Autores: Oliveira, Katia Soares de, Ferraz, Jefferson Jose Sodre, Leal, Mariana Ferreira [UNIFESP], Calcagno, Danielle Queiroz [UNIFESP], Seabra, Aline Damasceno, Khayat, Andre Salim, Montenegro, Raquel Carvalho, Alves, Ana Paula Negreiros Nunes, Assumpcao, Paulo Pimentel, Smith, Marilia de Arruda Cardoso [UNIFESP], Burbano, Rommel Rodriguez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1186/1471-230X-14-179
http://repositorio.unifesp.br/handle/11600/38330
Resumo: Background: Helicobacter pylori (HP) and Epstein-Barr virus (EBV) have been associated with cancer development. We evaluated the prevalence of HP, HP CagA(+) and EBV infection in gastric cancer (GC) samples from adults and in gastric tissues from patients who underwent upper endoscopy (UE).Methods: Samples from UE and GC were collected to investigate the presence of HP infection and the HP virulence factor CagA by a urease test and PCR. the presence of EBV was detected by Eber-1 in situ hybridization.Results: in UE, 85.5% of juvenile patients showed some degree of gastritis (45.3% of patients with mild gastritis and 54.7% with moderate/severe gastritis) and patients with mild gastritis were younger than patients with moderate/severe gastritis. Among adults, 48.7% presented mild gastritis and 51.3% moderate/severe gastritis. HP infection was detected in 0% of normal mucosa, 58.5% of juvenile gastritis patients, 69.2% of adult gastritis patients and 88% of GC patients. in these same groups, HP CagA(+) was detected in 0%, 37.7%, 61.5% and 67.2% of tissue samples, respectively. in juvenile patients, HP infection was more common in those with gastritis than in normal samples (p = 0.004). the patients with either HP or HP CagA(+) were older than patients without these pathogens (p < 0.05). in juvenile patients, HP infection was more frequent in cases of moderate/severe gastritis than in cases of mild gastritis (p = 0.026). Moreover, in patients with GC, HP infection was more frequent in males than in females (p = 0.023). GC patients with HP CagA(+) were older than patients with HP CagA-(p = 0.027). HP CagA(+) was more common in intestinal-type than diffuse-type GC (p = 0.012). HP CagA(+) was also associated with lymph-node (p = 0.024) and distal (p = 0.005) metastasis. No association between EBV infection and HP infection or any clinicopathological variable was detected.Conclusions: Our results suggest that HP is involved in the pathophysiology of severe gastric lesions and in the development of GC, particularly when CagA(+) is present. EBV was not the primary pathogenic factor in our samples.
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spelling Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern BrazilHelicobacter pyloriEpstein-Barr virusGastritisGastric cancerBackground: Helicobacter pylori (HP) and Epstein-Barr virus (EBV) have been associated with cancer development. We evaluated the prevalence of HP, HP CagA(+) and EBV infection in gastric cancer (GC) samples from adults and in gastric tissues from patients who underwent upper endoscopy (UE).Methods: Samples from UE and GC were collected to investigate the presence of HP infection and the HP virulence factor CagA by a urease test and PCR. the presence of EBV was detected by Eber-1 in situ hybridization.Results: in UE, 85.5% of juvenile patients showed some degree of gastritis (45.3% of patients with mild gastritis and 54.7% with moderate/severe gastritis) and patients with mild gastritis were younger than patients with moderate/severe gastritis. Among adults, 48.7% presented mild gastritis and 51.3% moderate/severe gastritis. HP infection was detected in 0% of normal mucosa, 58.5% of juvenile gastritis patients, 69.2% of adult gastritis patients and 88% of GC patients. in these same groups, HP CagA(+) was detected in 0%, 37.7%, 61.5% and 67.2% of tissue samples, respectively. in juvenile patients, HP infection was more common in those with gastritis than in normal samples (p = 0.004). the patients with either HP or HP CagA(+) were older than patients without these pathogens (p < 0.05). in juvenile patients, HP infection was more frequent in cases of moderate/severe gastritis than in cases of mild gastritis (p = 0.026). Moreover, in patients with GC, HP infection was more frequent in males than in females (p = 0.023). GC patients with HP CagA(+) were older than patients with HP CagA-(p = 0.027). HP CagA(+) was more common in intestinal-type than diffuse-type GC (p = 0.012). HP CagA(+) was also associated with lymph-node (p = 0.024) and distal (p = 0.005) metastasis. No association between EBV infection and HP infection or any clinicopathological variable was detected.Conclusions: Our results suggest that HP is involved in the pathophysiology of severe gastric lesions and in the development of GC, particularly when CagA(+) is present. EBV was not the primary pathogenic factor in our samples.Fed Univ Para, Inst Ciencias Biol, Lab Citogenet Humana, BR-66075110 Belem, PA, BrazilFed Univ Para, Inst Ciencias Saude, BR-66075110 Belem, PA, BrazilCtr Univ Para, Belem, PA, BrazilUniversidade Federal de São Paulo, Dept Ortopedia & Traumatol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, São Paulo, BrazilFed Univ Para, BR-66075110 Belem, PA, BrazilUniv Fed Ceara, Fac Odontol, Dept Oral Pathol, Fortaleza, CE, BrazilUniversidade Federal de São Paulo, Dept Ortopedia & Traumatol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morfol & Genet, Disciplina Genet, São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Biomed Central LtdFed Univ ParaCtr Univ ParaUniversidade Federal de São Paulo (UNIFESP)Univ Fed CearaSouza, Carolina Rosal Teixeira deOliveira, Katia Soares deFerraz, Jefferson Jose SodreLeal, Mariana Ferreira [UNIFESP]Calcagno, Danielle Queiroz [UNIFESP]Seabra, Aline DamascenoKhayat, Andre SalimMontenegro, Raquel CarvalhoAlves, Ana Paula Negreiros NunesAssumpcao, Paulo PimentelSmith, Marilia de Arruda Cardoso [UNIFESP]Burbano, Rommel Rodriguez2016-01-24T14:38:00Z2016-01-24T14:38:00Z2014-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9application/pdfhttp://dx.doi.org/10.1186/1471-230X-14-179Bmc Gastroenterology. London: Biomed Central Ltd, v. 14, 9 p., 2014.10.1186/1471-230X-14-179WOS000346673400001.pdf1471-230Xhttp://repositorio.unifesp.br/handle/11600/38330WOS:000346673400001engBmc Gastroenterologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T05:46:10Zoai:repositorio.unifesp.br/:11600/38330Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T05:46:10Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil
title Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil
spellingShingle Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil
Souza, Carolina Rosal Teixeira de
Helicobacter pylori
Epstein-Barr virus
Gastritis
Gastric cancer
title_short Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil
title_full Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil
title_fullStr Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil
title_full_unstemmed Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil
title_sort Occurrence of Helicobacter pylori and Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil
author Souza, Carolina Rosal Teixeira de
author_facet Souza, Carolina Rosal Teixeira de
Oliveira, Katia Soares de
Ferraz, Jefferson Jose Sodre
Leal, Mariana Ferreira [UNIFESP]
Calcagno, Danielle Queiroz [UNIFESP]
Seabra, Aline Damasceno
Khayat, Andre Salim
Montenegro, Raquel Carvalho
Alves, Ana Paula Negreiros Nunes
Assumpcao, Paulo Pimentel
Smith, Marilia de Arruda Cardoso [UNIFESP]
Burbano, Rommel Rodriguez
author_role author
author2 Oliveira, Katia Soares de
Ferraz, Jefferson Jose Sodre
Leal, Mariana Ferreira [UNIFESP]
Calcagno, Danielle Queiroz [UNIFESP]
Seabra, Aline Damasceno
Khayat, Andre Salim
Montenegro, Raquel Carvalho
Alves, Ana Paula Negreiros Nunes
Assumpcao, Paulo Pimentel
Smith, Marilia de Arruda Cardoso [UNIFESP]
Burbano, Rommel Rodriguez
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fed Univ Para
Ctr Univ Para
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Ceara
dc.contributor.author.fl_str_mv Souza, Carolina Rosal Teixeira de
Oliveira, Katia Soares de
Ferraz, Jefferson Jose Sodre
Leal, Mariana Ferreira [UNIFESP]
Calcagno, Danielle Queiroz [UNIFESP]
Seabra, Aline Damasceno
Khayat, Andre Salim
Montenegro, Raquel Carvalho
Alves, Ana Paula Negreiros Nunes
Assumpcao, Paulo Pimentel
Smith, Marilia de Arruda Cardoso [UNIFESP]
Burbano, Rommel Rodriguez
dc.subject.por.fl_str_mv Helicobacter pylori
Epstein-Barr virus
Gastritis
Gastric cancer
topic Helicobacter pylori
Epstein-Barr virus
Gastritis
Gastric cancer
description Background: Helicobacter pylori (HP) and Epstein-Barr virus (EBV) have been associated with cancer development. We evaluated the prevalence of HP, HP CagA(+) and EBV infection in gastric cancer (GC) samples from adults and in gastric tissues from patients who underwent upper endoscopy (UE).Methods: Samples from UE and GC were collected to investigate the presence of HP infection and the HP virulence factor CagA by a urease test and PCR. the presence of EBV was detected by Eber-1 in situ hybridization.Results: in UE, 85.5% of juvenile patients showed some degree of gastritis (45.3% of patients with mild gastritis and 54.7% with moderate/severe gastritis) and patients with mild gastritis were younger than patients with moderate/severe gastritis. Among adults, 48.7% presented mild gastritis and 51.3% moderate/severe gastritis. HP infection was detected in 0% of normal mucosa, 58.5% of juvenile gastritis patients, 69.2% of adult gastritis patients and 88% of GC patients. in these same groups, HP CagA(+) was detected in 0%, 37.7%, 61.5% and 67.2% of tissue samples, respectively. in juvenile patients, HP infection was more common in those with gastritis than in normal samples (p = 0.004). the patients with either HP or HP CagA(+) were older than patients without these pathogens (p < 0.05). in juvenile patients, HP infection was more frequent in cases of moderate/severe gastritis than in cases of mild gastritis (p = 0.026). Moreover, in patients with GC, HP infection was more frequent in males than in females (p = 0.023). GC patients with HP CagA(+) were older than patients with HP CagA-(p = 0.027). HP CagA(+) was more common in intestinal-type than diffuse-type GC (p = 0.012). HP CagA(+) was also associated with lymph-node (p = 0.024) and distal (p = 0.005) metastasis. No association between EBV infection and HP infection or any clinicopathological variable was detected.Conclusions: Our results suggest that HP is involved in the pathophysiology of severe gastric lesions and in the development of GC, particularly when CagA(+) is present. EBV was not the primary pathogenic factor in our samples.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-15
2016-01-24T14:38:00Z
2016-01-24T14:38:00Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1471-230X-14-179
Bmc Gastroenterology. London: Biomed Central Ltd, v. 14, 9 p., 2014.
10.1186/1471-230X-14-179
WOS000346673400001.pdf
1471-230X
http://repositorio.unifesp.br/handle/11600/38330
WOS:000346673400001
url http://dx.doi.org/10.1186/1471-230X-14-179
http://repositorio.unifesp.br/handle/11600/38330
identifier_str_mv Bmc Gastroenterology. London: Biomed Central Ltd, v. 14, 9 p., 2014.
10.1186/1471-230X-14-179
WOS000346673400001.pdf
1471-230X
WOS:000346673400001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bmc Gastroenterology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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