Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis

Detalhes bibliográficos
Autor(a) principal: Borsanelli, Ana C.
Data de Publicação: 2022
Outros Autores: Athayde, Flávia R. F. [UNESP], Riggio, Marcello P., Brandt, Bernd W., Rocha, Fernando I., Jesus, Ederson C., Gaetti-Jardim, Elerson [UNESP], Schweitzer, Christiane M. [UNESP], Dutra, Iveraldo S. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fmicb.2022.936021
http://hdl.handle.net/11449/241566
Resumo: Extensive cattle livestock is advancing in Amazonia and its low productivity, with consequent pressure to open new areas, is partly due to sanitary problems and, among them, the periodontal diseases, whose environmental triggers or modifying factors are unknown. In this study, we used high-throughput sequencing, network analysis and predicted functions to investigate the dental and ruminal microbiota of cattle raised in new livestock areas in the Amazon and identify possible keystone pathogens and proteins associated with the disease. Ninety-three genera were common in dental and ruminal fluid microbiomes and among them periodontal pathogens such as Fusobacterium, Prevotella, Porphyromonas and Actinomyces were recognized. Network analysis showed that dental microbiomes of clinically healthy animals tend to comprise a group of OTUs in homeostasis and when analyzed together, dental and ruminal fluid microbiomes of animals with periodontitis had almost twice the number of negative edges, indicating possible competition between bacteria and dysbiosis. The incisor dental and ruminal fluid microbiomes were dominated by a core community composed of members of the phyla Firmicutes and Bacteroidetes. Network results showed that members of the Prevotella genus stood out among the top five OTUs, with the largest number of hubs in the dental and ruminal microbiota of animals with periodontitis. Protein families linked to an inflammatory environment were predicted in the dental and ruminal microbiota of cattle with periodontitis. The dissimilarity between dental microbiomes, discriminating between healthy cattle and those with periodontitis and the identification of possible key pathogens, represent an important reference to elucidate the triggers involved in the etiopathogenesis of bovine periodontitis, and possibly in the development of measures to control the disease and reduce the pressures for deforestation.
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spelling Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitisAmazonbiodiversitybovinedental microbiomeperiodontitisPrevotellaruminal fluid microbiomeExtensive cattle livestock is advancing in Amazonia and its low productivity, with consequent pressure to open new areas, is partly due to sanitary problems and, among them, the periodontal diseases, whose environmental triggers or modifying factors are unknown. In this study, we used high-throughput sequencing, network analysis and predicted functions to investigate the dental and ruminal microbiota of cattle raised in new livestock areas in the Amazon and identify possible keystone pathogens and proteins associated with the disease. Ninety-three genera were common in dental and ruminal fluid microbiomes and among them periodontal pathogens such as Fusobacterium, Prevotella, Porphyromonas and Actinomyces were recognized. Network analysis showed that dental microbiomes of clinically healthy animals tend to comprise a group of OTUs in homeostasis and when analyzed together, dental and ruminal fluid microbiomes of animals with periodontitis had almost twice the number of negative edges, indicating possible competition between bacteria and dysbiosis. The incisor dental and ruminal fluid microbiomes were dominated by a core community composed of members of the phyla Firmicutes and Bacteroidetes. Network results showed that members of the Prevotella genus stood out among the top five OTUs, with the largest number of hubs in the dental and ruminal microbiota of animals with periodontitis. Protein families linked to an inflammatory environment were predicted in the dental and ruminal microbiota of cattle with periodontitis. The dissimilarity between dental microbiomes, discriminating between healthy cattle and those with periodontitis and the identification of possible key pathogens, represent an important reference to elucidate the triggers involved in the etiopathogenesis of bovine periodontitis, and possibly in the development of measures to control the disease and reduce the pressures for deforestation.National Academies of Sciences, Engineering, and MedicineDepartment of Veterinary Medicine School of Veterinary Medicine and Animal Science Universidade Federal de Goiás (UFG), GoiásDepartment of Production and Animal Health School of Veterinary Medicine São Paulo State University (Unesp)Dental School University of GlasgowDepartment of Preventive Dentistry Academic Centre for Dentistry Amsterdam University of Amsterdam and VU University AmsterdamDepartment of Soil Universidade Federal Rural do Rio de Janeiro, Rio de JaneiroNational Agrobiology Research Center Embrapa AgrobiologiaDepartment of Diagnosis and Surgery Dental School São Paulo State University (Unesp)Department of Mathematics School of Engineering São Paulo State University (Unesp), Ilha SolteiraDepartment of Production and Animal Health School of Veterinary Medicine São Paulo State University (Unesp)Department of Diagnosis and Surgery Dental School São Paulo State University (Unesp)Department of Mathematics School of Engineering São Paulo State University (Unesp), Ilha SolteiraUniversidade Federal de Goiás (UFG)Universidade Estadual Paulista (UNESP)University of GlasgowUniversity of Amsterdam and VU University AmsterdamUniversidade Federal Rural do Rio de JaneiroEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Borsanelli, Ana C.Athayde, Flávia R. F. [UNESP]Riggio, Marcello P.Brandt, Bernd W.Rocha, Fernando I.Jesus, Ederson C.Gaetti-Jardim, Elerson [UNESP]Schweitzer, Christiane M. [UNESP]Dutra, Iveraldo S. [UNESP]2023-03-01T21:10:26Z2023-03-01T21:10:26Z2022-08-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2022.936021Frontiers in Microbiology, v. 13.1664-302Xhttp://hdl.handle.net/11449/24156610.3389/fmicb.2022.9360212-s2.0-85136815299Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2024-07-10T15:41:36Zoai:repositorio.unesp.br:11449/241566Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:42:40.099626Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis
title Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis
spellingShingle Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis
Borsanelli, Ana C.
Amazon
biodiversity
bovine
dental microbiome
periodontitis
Prevotella
ruminal fluid microbiome
title_short Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis
title_full Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis
title_fullStr Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis
title_full_unstemmed Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis
title_sort Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis
author Borsanelli, Ana C.
author_facet Borsanelli, Ana C.
Athayde, Flávia R. F. [UNESP]
Riggio, Marcello P.
Brandt, Bernd W.
Rocha, Fernando I.
Jesus, Ederson C.
Gaetti-Jardim, Elerson [UNESP]
Schweitzer, Christiane M. [UNESP]
Dutra, Iveraldo S. [UNESP]
author_role author
author2 Athayde, Flávia R. F. [UNESP]
Riggio, Marcello P.
Brandt, Bernd W.
Rocha, Fernando I.
Jesus, Ederson C.
Gaetti-Jardim, Elerson [UNESP]
Schweitzer, Christiane M. [UNESP]
Dutra, Iveraldo S. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Goiás (UFG)
Universidade Estadual Paulista (UNESP)
University of Glasgow
University of Amsterdam and VU University Amsterdam
Universidade Federal Rural do Rio de Janeiro
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Borsanelli, Ana C.
Athayde, Flávia R. F. [UNESP]
Riggio, Marcello P.
Brandt, Bernd W.
Rocha, Fernando I.
Jesus, Ederson C.
Gaetti-Jardim, Elerson [UNESP]
Schweitzer, Christiane M. [UNESP]
Dutra, Iveraldo S. [UNESP]
dc.subject.por.fl_str_mv Amazon
biodiversity
bovine
dental microbiome
periodontitis
Prevotella
ruminal fluid microbiome
topic Amazon
biodiversity
bovine
dental microbiome
periodontitis
Prevotella
ruminal fluid microbiome
description Extensive cattle livestock is advancing in Amazonia and its low productivity, with consequent pressure to open new areas, is partly due to sanitary problems and, among them, the periodontal diseases, whose environmental triggers or modifying factors are unknown. In this study, we used high-throughput sequencing, network analysis and predicted functions to investigate the dental and ruminal microbiota of cattle raised in new livestock areas in the Amazon and identify possible keystone pathogens and proteins associated with the disease. Ninety-three genera were common in dental and ruminal fluid microbiomes and among them periodontal pathogens such as Fusobacterium, Prevotella, Porphyromonas and Actinomyces were recognized. Network analysis showed that dental microbiomes of clinically healthy animals tend to comprise a group of OTUs in homeostasis and when analyzed together, dental and ruminal fluid microbiomes of animals with periodontitis had almost twice the number of negative edges, indicating possible competition between bacteria and dysbiosis. The incisor dental and ruminal fluid microbiomes were dominated by a core community composed of members of the phyla Firmicutes and Bacteroidetes. Network results showed that members of the Prevotella genus stood out among the top five OTUs, with the largest number of hubs in the dental and ruminal microbiota of animals with periodontitis. Protein families linked to an inflammatory environment were predicted in the dental and ruminal microbiota of cattle with periodontitis. The dissimilarity between dental microbiomes, discriminating between healthy cattle and those with periodontitis and the identification of possible key pathogens, represent an important reference to elucidate the triggers involved in the etiopathogenesis of bovine periodontitis, and possibly in the development of measures to control the disease and reduce the pressures for deforestation.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-12
2023-03-01T21:10:26Z
2023-03-01T21:10:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fmicb.2022.936021
Frontiers in Microbiology, v. 13.
1664-302X
http://hdl.handle.net/11449/241566
10.3389/fmicb.2022.936021
2-s2.0-85136815299
url http://dx.doi.org/10.3389/fmicb.2022.936021
http://hdl.handle.net/11449/241566
identifier_str_mv Frontiers in Microbiology, v. 13.
1664-302X
10.3389/fmicb.2022.936021
2-s2.0-85136815299
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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