OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE

Detalhes bibliográficos
Autor(a) principal: Silva, Jailson Do Nascimento
Data de Publicação: 2023
Outros Autores: Linhares, Amanda Camila Dos Santos, Palmieri, Dario Abel [UNESP], Costa, Marcones Ferreira, de SÁ, Gisele Holanda, Gomes, Maria Fernanda da Costa, Dos Santos, Michelli Ferreira, Feitoza, Lidiane de Lima, Valente, Sérgio Emílio Dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.14393/BJ-v39n0a2023-62577
http://hdl.handle.net/11449/249748
Resumo: Molecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies for endangered populations. The different molecular techniques involve the evaluation of many individuals; therefore, it is crucial to have fast, efficient, and inexpensive methods for DNA extraction. Given the importance of the Aroeira (Myracrodruon urundeuva Fr. All.) it is pertinent to optimize a protocol that allows the obtainment of intact and pure DNA, aiming to assist conservation strategies for this species that is threatened with extinction. Thus, this study aimed to compare five DNA extraction methods: Dellaporta et al. (1983), Doyle and Doyle (1987) modified, Ferreira and Grattapaglia (1995), Romano and Brasileiro (2015), and Khanuja et al. (1999) and optimize the most efficient protocol for M. urundeuva. The modified DNA extraction protocol proposed by Doyle and Doyle (1987), using 100 mg of leaf tissue and 6 µl of β-mercaptoethanol was the protocol that presented the sharpest bands after DNA electrophoresis and after the reactions of amplification employing Polymerase Chain Reaction (PCR). Therefore, it is suggested to use the protocol described by Doyle and Doyle (1987) modified for the extraction of DNA from young M. urundeuva leaves to carry out techniques involving molecular markers.
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spelling OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREECTABDNA isolationMyracrodruon urundeuvaMolecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies for endangered populations. The different molecular techniques involve the evaluation of many individuals; therefore, it is crucial to have fast, efficient, and inexpensive methods for DNA extraction. Given the importance of the Aroeira (Myracrodruon urundeuva Fr. All.) it is pertinent to optimize a protocol that allows the obtainment of intact and pure DNA, aiming to assist conservation strategies for this species that is threatened with extinction. Thus, this study aimed to compare five DNA extraction methods: Dellaporta et al. (1983), Doyle and Doyle (1987) modified, Ferreira and Grattapaglia (1995), Romano and Brasileiro (2015), and Khanuja et al. (1999) and optimize the most efficient protocol for M. urundeuva. The modified DNA extraction protocol proposed by Doyle and Doyle (1987), using 100 mg of leaf tissue and 6 µl of β-mercaptoethanol was the protocol that presented the sharpest bands after DNA electrophoresis and after the reactions of amplification employing Polymerase Chain Reaction (PCR). Therefore, it is suggested to use the protocol described by Doyle and Doyle (1987) modified for the extraction of DNA from young M. urundeuva leaves to carry out techniques involving molecular markers.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Education Instituto Federal do Piauí, PiauíDepartment of Biology Universidade Federal do Piauí, PiauíDepartment of Biotechnology Universidade Estadual Paulista (UNESP), São PauloUniversidade Federal do Piauí, PiauíPostgraduate Program in Genetics and Breeding Universidade Federal do Piauí, PiauíDepartment of Biology Universidade Estadual do Piauí, PiauíDepartment of Biotechnology Universidade Estadual Paulista (UNESP), São PauloInstituto Federal do PiauíUniversidade Federal do PiauíUniversidade Estadual Paulista (UNESP)Universidade Estadual do PiauíSilva, Jailson Do NascimentoLinhares, Amanda Camila Dos SantosPalmieri, Dario Abel [UNESP]Costa, Marcones Ferreirade SÁ, Gisele HolandaGomes, Maria Fernanda da CostaDos Santos, Michelli FerreiraFeitoza, Lidiane de LimaValente, Sérgio Emílio Dos Santos2023-07-29T16:08:06Z2023-07-29T16:08:06Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.14393/BJ-v39n0a2023-62577Bioscience Journal, v. 39.1981-31631516-3725http://hdl.handle.net/11449/24974810.14393/BJ-v39n0a2023-625772-s2.0-85149714071Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioscience Journalinfo:eu-repo/semantics/openAccess2024-06-13T17:39:05Zoai:repositorio.unesp.br:11449/249748Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:58:37.231526Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE
title OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE
spellingShingle OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE
Silva, Jailson Do Nascimento
CTAB
DNA isolation
Myracrodruon urundeuva
title_short OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE
title_full OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE
title_fullStr OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE
title_full_unstemmed OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE
title_sort OPTIMIZED METHOD FOR DNA EXTRACTION AND PCR AMPLIFICATION IN AROEIRA TREE
author Silva, Jailson Do Nascimento
author_facet Silva, Jailson Do Nascimento
Linhares, Amanda Camila Dos Santos
Palmieri, Dario Abel [UNESP]
Costa, Marcones Ferreira
de SÁ, Gisele Holanda
Gomes, Maria Fernanda da Costa
Dos Santos, Michelli Ferreira
Feitoza, Lidiane de Lima
Valente, Sérgio Emílio Dos Santos
author_role author
author2 Linhares, Amanda Camila Dos Santos
Palmieri, Dario Abel [UNESP]
Costa, Marcones Ferreira
de SÁ, Gisele Holanda
Gomes, Maria Fernanda da Costa
Dos Santos, Michelli Ferreira
Feitoza, Lidiane de Lima
Valente, Sérgio Emílio Dos Santos
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Federal do Piauí
Universidade Federal do Piauí
Universidade Estadual Paulista (UNESP)
Universidade Estadual do Piauí
dc.contributor.author.fl_str_mv Silva, Jailson Do Nascimento
Linhares, Amanda Camila Dos Santos
Palmieri, Dario Abel [UNESP]
Costa, Marcones Ferreira
de SÁ, Gisele Holanda
Gomes, Maria Fernanda da Costa
Dos Santos, Michelli Ferreira
Feitoza, Lidiane de Lima
Valente, Sérgio Emílio Dos Santos
dc.subject.por.fl_str_mv CTAB
DNA isolation
Myracrodruon urundeuva
topic CTAB
DNA isolation
Myracrodruon urundeuva
description Molecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies for endangered populations. The different molecular techniques involve the evaluation of many individuals; therefore, it is crucial to have fast, efficient, and inexpensive methods for DNA extraction. Given the importance of the Aroeira (Myracrodruon urundeuva Fr. All.) it is pertinent to optimize a protocol that allows the obtainment of intact and pure DNA, aiming to assist conservation strategies for this species that is threatened with extinction. Thus, this study aimed to compare five DNA extraction methods: Dellaporta et al. (1983), Doyle and Doyle (1987) modified, Ferreira and Grattapaglia (1995), Romano and Brasileiro (2015), and Khanuja et al. (1999) and optimize the most efficient protocol for M. urundeuva. The modified DNA extraction protocol proposed by Doyle and Doyle (1987), using 100 mg of leaf tissue and 6 µl of β-mercaptoethanol was the protocol that presented the sharpest bands after DNA electrophoresis and after the reactions of amplification employing Polymerase Chain Reaction (PCR). Therefore, it is suggested to use the protocol described by Doyle and Doyle (1987) modified for the extraction of DNA from young M. urundeuva leaves to carry out techniques involving molecular markers.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T16:08:06Z
2023-07-29T16:08:06Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.14393/BJ-v39n0a2023-62577
Bioscience Journal, v. 39.
1981-3163
1516-3725
http://hdl.handle.net/11449/249748
10.14393/BJ-v39n0a2023-62577
2-s2.0-85149714071
url http://dx.doi.org/10.14393/BJ-v39n0a2023-62577
http://hdl.handle.net/11449/249748
identifier_str_mv Bioscience Journal, v. 39.
1981-3163
1516-3725
10.14393/BJ-v39n0a2023-62577
2-s2.0-85149714071
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioscience Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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