Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individuals
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1477-7827-11-101 http://hdl.handle.net/11449/112271 |
Resumo: | Background: Intrauterine insemination (IUI) is widely used to treat infertility, and its adequate indication is important to obtain good pregnancy rates. To assess which couples could benefit from IUI, this study aimed to evaluate whether sperm motility using a discontinuous gradient of different densities and incubation in CO2 in normospermic individuals is able to predict pregnancy.Methods: A total of 175 couples underwent 175 IUI cycles. The inclusion criteria for women were as follows: 35 years old or younger (age range: from 27 to 35 years) with normal fallopian tubes; endometriosis grades I-II; unexplained infertility; nonhyperandrogenic ovulatory dysfunction. Men with normal seminal parameters were also included. All patients underwent ovarian stimulation with clomiphene citrate and human hMG or r-FSH. When one or (at most) three follicles measuring 18 to 20 mm were observed, hCG (5000 UI) or r-hCG (250 mcg) was administered and IUI performed 36-40 h after hCG. Sperm processing was performed using a discontinuous concentration gradient. A 20 microliters aliquot was incubated for 24 h at 37 degrees C in 5% CO2 following a total progressive motility analysis. The Mann-Whitney and Chi-square tests, as well as a ROC curve were used to determine the cutoff value for motility.Results: Of the 175 couples, 52 (in 52 IUI cycles) achieved clinical pregnancies (CP rate per cycle: 29.7%). The analysis of age, duration and causes of infertility did not indicate any statistical significance between pregnancy and no pregnancy groups, similar to the results for total sperm count and morphology analyses, excluding progressive motility (p < 0.0001). The comparison of progressive motility after processing and 24 h after incubation between these two groups indicated that progressive motility 24 h after incubation was higher in the pregnancy group. The analysis of the progressive motility of the pregnancy group after processing and 24 h after incubation has not shown any motility difference at 24 h after incubation; additionally, in couples who did not obtain pregnancy, there was a statistically significant decrease in progressive motility 24 h after incubation (p < 0.0001). The ROC curve analysis generated a cutoff value of 56.5% for progressive motility at 24 h after incubation and this cutoff value produced 96.1% sensitivity, 92.7% specificity, 84.7% positive predictive value and 98.3% negative predictive value.Conclusions: We concluded that the sperm motility of normospermic individuals 24 h after incubation at 37 degrees C in 5% CO2, with a cutoff value of 56.5%, is predictive of IUI success. |
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Repositório Institucional da UNESP |
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Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individualsSperm motilitySuccessful inseminationIntrauterine inseminationSperm survivalAdvanced semen analysisBackground: Intrauterine insemination (IUI) is widely used to treat infertility, and its adequate indication is important to obtain good pregnancy rates. To assess which couples could benefit from IUI, this study aimed to evaluate whether sperm motility using a discontinuous gradient of different densities and incubation in CO2 in normospermic individuals is able to predict pregnancy.Methods: A total of 175 couples underwent 175 IUI cycles. The inclusion criteria for women were as follows: 35 years old or younger (age range: from 27 to 35 years) with normal fallopian tubes; endometriosis grades I-II; unexplained infertility; nonhyperandrogenic ovulatory dysfunction. Men with normal seminal parameters were also included. All patients underwent ovarian stimulation with clomiphene citrate and human hMG or r-FSH. When one or (at most) three follicles measuring 18 to 20 mm were observed, hCG (5000 UI) or r-hCG (250 mcg) was administered and IUI performed 36-40 h after hCG. Sperm processing was performed using a discontinuous concentration gradient. A 20 microliters aliquot was incubated for 24 h at 37 degrees C in 5% CO2 following a total progressive motility analysis. The Mann-Whitney and Chi-square tests, as well as a ROC curve were used to determine the cutoff value for motility.Results: Of the 175 couples, 52 (in 52 IUI cycles) achieved clinical pregnancies (CP rate per cycle: 29.7%). The analysis of age, duration and causes of infertility did not indicate any statistical significance between pregnancy and no pregnancy groups, similar to the results for total sperm count and morphology analyses, excluding progressive motility (p < 0.0001). The comparison of progressive motility after processing and 24 h after incubation between these two groups indicated that progressive motility 24 h after incubation was higher in the pregnancy group. The analysis of the progressive motility of the pregnancy group after processing and 24 h after incubation has not shown any motility difference at 24 h after incubation; additionally, in couples who did not obtain pregnancy, there was a statistically significant decrease in progressive motility 24 h after incubation (p < 0.0001). The ROC curve analysis generated a cutoff value of 56.5% for progressive motility at 24 h after incubation and this cutoff value produced 96.1% sensitivity, 92.7% specificity, 84.7% positive predictive value and 98.3% negative predictive value.Conclusions: We concluded that the sperm motility of normospermic individuals 24 h after incubation at 37 degrees C in 5% CO2, with a cutoff value of 56.5%, is predictive of IUI success.Ctr Human Reprod Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Dept Gynaecol & Obstet, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Bioestat, Botucatu, SP, BrazilCtr Human Reprod Prof Franco Jr, Ribeirao Preto, SP, BrazilPaulista Ctr Diag Res & Training, Ribeirao Preto, SP, BrazilGERAR Fertil Clin, Recife, PE, BrazilSao Paulo State Univ UNESP, Botucatu Med Sch, Dept Gynaecol & Obstet, Botucatu, SP, BrazilSao Paulo State Univ UNESP, Inst Biosci Botucatu, Dept Bioestat, Botucatu, SP, BrazilBiomed Central Ltd.Ctr Human Reprod Sao Jose do Rio PretoUniversidade Estadual Paulista (Unesp)Ctr Human Reprod Prof Franco JrPaulista Ctr Diag Res & TrainingGERAR Fertil ClinAraujo, Ligia F. P. de [UNESP]Araujo Filho, Edilberto deFacio, Cassio L.Bossoni, Marcia C. O.Machado-Paula, Ligiane A.Corrente, José Eduardo [UNESP]Cavagna, MarioMatheus, Paulo C. S.Pontes, Anaglória [UNESP]2014-12-03T13:10:34Z2014-12-03T13:10:34Z2013-10-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1186/1477-7827-11-101Reproductive Biology And Endocrinology. London: Biomed Central Ltd, v. 11, 7 p., 2013.1477-7827http://hdl.handle.net/11449/11227110.1186/1477-7827-11-101WOS:000326242800001WOS000326242800001.pdf05141786546676840000-0001-5478-4996Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproductive Biology and Endocrinology2.8521,203info:eu-repo/semantics/openAccess2024-08-16T14:12:38Zoai:repositorio.unesp.br:11449/112271Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:12:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individuals |
title |
Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individuals |
spellingShingle |
Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individuals Araujo, Ligia F. P. de [UNESP] Sperm motility Successful insemination Intrauterine insemination Sperm survival Advanced semen analysis |
title_short |
Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individuals |
title_full |
Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individuals |
title_fullStr |
Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individuals |
title_full_unstemmed |
Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individuals |
title_sort |
Efficacy of sperm motility after processing and incubation to predict pregnancy after intrauterine insemination in normospermic individuals |
author |
Araujo, Ligia F. P. de [UNESP] |
author_facet |
Araujo, Ligia F. P. de [UNESP] Araujo Filho, Edilberto de Facio, Cassio L. Bossoni, Marcia C. O. Machado-Paula, Ligiane A. Corrente, José Eduardo [UNESP] Cavagna, Mario Matheus, Paulo C. S. Pontes, Anaglória [UNESP] |
author_role |
author |
author2 |
Araujo Filho, Edilberto de Facio, Cassio L. Bossoni, Marcia C. O. Machado-Paula, Ligiane A. Corrente, José Eduardo [UNESP] Cavagna, Mario Matheus, Paulo C. S. Pontes, Anaglória [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Ctr Human Reprod Sao Jose do Rio Preto Universidade Estadual Paulista (Unesp) Ctr Human Reprod Prof Franco Jr Paulista Ctr Diag Res & Training GERAR Fertil Clin |
dc.contributor.author.fl_str_mv |
Araujo, Ligia F. P. de [UNESP] Araujo Filho, Edilberto de Facio, Cassio L. Bossoni, Marcia C. O. Machado-Paula, Ligiane A. Corrente, José Eduardo [UNESP] Cavagna, Mario Matheus, Paulo C. S. Pontes, Anaglória [UNESP] |
dc.subject.por.fl_str_mv |
Sperm motility Successful insemination Intrauterine insemination Sperm survival Advanced semen analysis |
topic |
Sperm motility Successful insemination Intrauterine insemination Sperm survival Advanced semen analysis |
description |
Background: Intrauterine insemination (IUI) is widely used to treat infertility, and its adequate indication is important to obtain good pregnancy rates. To assess which couples could benefit from IUI, this study aimed to evaluate whether sperm motility using a discontinuous gradient of different densities and incubation in CO2 in normospermic individuals is able to predict pregnancy.Methods: A total of 175 couples underwent 175 IUI cycles. The inclusion criteria for women were as follows: 35 years old or younger (age range: from 27 to 35 years) with normal fallopian tubes; endometriosis grades I-II; unexplained infertility; nonhyperandrogenic ovulatory dysfunction. Men with normal seminal parameters were also included. All patients underwent ovarian stimulation with clomiphene citrate and human hMG or r-FSH. When one or (at most) three follicles measuring 18 to 20 mm were observed, hCG (5000 UI) or r-hCG (250 mcg) was administered and IUI performed 36-40 h after hCG. Sperm processing was performed using a discontinuous concentration gradient. A 20 microliters aliquot was incubated for 24 h at 37 degrees C in 5% CO2 following a total progressive motility analysis. The Mann-Whitney and Chi-square tests, as well as a ROC curve were used to determine the cutoff value for motility.Results: Of the 175 couples, 52 (in 52 IUI cycles) achieved clinical pregnancies (CP rate per cycle: 29.7%). The analysis of age, duration and causes of infertility did not indicate any statistical significance between pregnancy and no pregnancy groups, similar to the results for total sperm count and morphology analyses, excluding progressive motility (p < 0.0001). The comparison of progressive motility after processing and 24 h after incubation between these two groups indicated that progressive motility 24 h after incubation was higher in the pregnancy group. The analysis of the progressive motility of the pregnancy group after processing and 24 h after incubation has not shown any motility difference at 24 h after incubation; additionally, in couples who did not obtain pregnancy, there was a statistically significant decrease in progressive motility 24 h after incubation (p < 0.0001). The ROC curve analysis generated a cutoff value of 56.5% for progressive motility at 24 h after incubation and this cutoff value produced 96.1% sensitivity, 92.7% specificity, 84.7% positive predictive value and 98.3% negative predictive value.Conclusions: We concluded that the sperm motility of normospermic individuals 24 h after incubation at 37 degrees C in 5% CO2, with a cutoff value of 56.5%, is predictive of IUI success. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-22 2014-12-03T13:10:34Z 2014-12-03T13:10:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1477-7827-11-101 Reproductive Biology And Endocrinology. London: Biomed Central Ltd, v. 11, 7 p., 2013. 1477-7827 http://hdl.handle.net/11449/112271 10.1186/1477-7827-11-101 WOS:000326242800001 WOS000326242800001.pdf 0514178654667684 0000-0001-5478-4996 |
url |
http://dx.doi.org/10.1186/1477-7827-11-101 http://hdl.handle.net/11449/112271 |
identifier_str_mv |
Reproductive Biology And Endocrinology. London: Biomed Central Ltd, v. 11, 7 p., 2013. 1477-7827 10.1186/1477-7827-11-101 WOS:000326242800001 WOS000326242800001.pdf 0514178654667684 0000-0001-5478-4996 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproductive Biology and Endocrinology 2.852 1,203 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128184758042624 |