CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVES

Detalhes bibliográficos
Autor(a) principal: De Sousa, Frank Bruno Vieira
Data de Publicação: 2023
Outros Autores: Cruz, Állefe Barbosa, Soares, Daniela Francisca, Cunha, Camila Luiza [UNESP], Holzbach, Juliana C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.14393/BJ-v39n0a2023-62323
http://hdl.handle.net/11449/246745
Resumo: Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.
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spelling CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVESArtemia salinaCerradoKaempferitrinClitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.Financiadora de Estudos e ProjetosUniversidade Federal do Tocantins, TocantinsUniversidade Estadual Paulista, São PauloUniversidade Estadual Paulista, São PauloFinanciadora de Estudos e Projetos: 01.08.0453.07Universidade Federal do TocantinsUniversidade Estadual Paulista (UNESP)De Sousa, Frank Bruno VieiraCruz, Állefe BarbosaSoares, Daniela FranciscaCunha, Camila Luiza [UNESP]Holzbach, Juliana C.2023-07-29T12:49:24Z2023-07-29T12:49:24Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.14393/BJ-v39n0a2023-62323Bioscience Journal, v. 39.1981-31631516-3725http://hdl.handle.net/11449/24674510.14393/BJ-v39n0a2023-623232-s2.0-85147183854Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioscience Journalinfo:eu-repo/semantics/openAccess2023-07-29T12:49:24Zoai:repositorio.unesp.br:11449/246745Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:04:12.017119Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVES
title CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVES
spellingShingle CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVES
De Sousa, Frank Bruno Vieira
Artemia salina
Cerrado
Kaempferitrin
title_short CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVES
title_full CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVES
title_fullStr CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVES
title_full_unstemmed CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVES
title_sort CHEMICAL CONSTITUENTS AND IN VIVO PRELIMINARY EVALUATION OF THE TOXICOLOGICAL ACTIVITY OF Ouratea spectabilis (OCHNACEAE) AND Clitoria guianensis (FABACEAE) LEAVES
author De Sousa, Frank Bruno Vieira
author_facet De Sousa, Frank Bruno Vieira
Cruz, Állefe Barbosa
Soares, Daniela Francisca
Cunha, Camila Luiza [UNESP]
Holzbach, Juliana C.
author_role author
author2 Cruz, Állefe Barbosa
Soares, Daniela Francisca
Cunha, Camila Luiza [UNESP]
Holzbach, Juliana C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Tocantins
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv De Sousa, Frank Bruno Vieira
Cruz, Állefe Barbosa
Soares, Daniela Francisca
Cunha, Camila Luiza [UNESP]
Holzbach, Juliana C.
dc.subject.por.fl_str_mv Artemia salina
Cerrado
Kaempferitrin
topic Artemia salina
Cerrado
Kaempferitrin
description Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD50) of 43.7, 25.4, and 233.4 mg.L−1, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD50 value of 115.13 mg.L−1.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T12:49:24Z
2023-07-29T12:49:24Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.14393/BJ-v39n0a2023-62323
Bioscience Journal, v. 39.
1981-3163
1516-3725
http://hdl.handle.net/11449/246745
10.14393/BJ-v39n0a2023-62323
2-s2.0-85147183854
url http://dx.doi.org/10.14393/BJ-v39n0a2023-62323
http://hdl.handle.net/11449/246745
identifier_str_mv Bioscience Journal, v. 39.
1981-3163
1516-3725
10.14393/BJ-v39n0a2023-62323
2-s2.0-85147183854
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioscience Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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