Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?

Detalhes bibliográficos
Autor(a) principal: Trevizan, Juliane Teramachi [UNESP]
Data de Publicação: 2018
Outros Autores: Carreira, Janaina Torres [UNESP], Carvalho, Isadora Resende [UNESP], Kipper, Bruna Helena [UNESP], Nagata, Walter Bertequine [UNESP], Perri, Silvia Helena Venturoli [UNESP], Franco Oliveira, Maria Emilia [UNESP], Pierucci, Julia Cestari [UNESP], Koivisto, Marion Burkhardt de [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.anireprosci.2018.04.071
http://hdl.handle.net/11449/179853
Resumo: The aims of this study were to evaluate cryopreserved semen of Nellore bulls of different ages and verify whether sperm quality declines with advancing age and whether lipid peroxidation and DNA damage are involved in this process. For this purpose, 40 Nellore bulls were divided into three age groups: Young, aged 1.8–2 years (n = 9); Adult, aged 3.5–7.0 years (n = 19); and Seniors, aged 8.0–14.3 years (n = 12). Three ejaculates were collected from each bull, cryopreserved and evaluated for various parameters including membrane integrity, mitochondrial potential (FITC-PSA and JC1), lipid peroxidation (C-11BODIPY 581 / 591) and oxidative DNA damage (8OHdG) using flow cytometry. The thawed semen of senior bulls was characterized by a low percentage of motile sperm (33.7 ± 6.1%), higher damage to the plasma and acrosomal membrane (37.5 ± 9.8%), and low mitochondrial potential (29.1 ± 13.8%), as well as higher percentages of peroxidated cells (53.6 ± 12.2%) and DNA damage (44.1 ± 11.0%; P < 0.05). Lipid peroxidation was negatively correlated with motility (r = −0.35, P < 0.0002), average mitochondrial potential (r = −0.42; P < 0.0001) and showed a positive correlation with membrane injury and oxidative DNA damage (r = 039; P = 0.0003). Young bulls presented superior thawed sperm quality, possibly due to greater resistance to oxidative stress and, consequently, to cryopreservation. In conclusion, the sperm quality of bull semen declines with advancing age and is strongly associated with increased oxidative damage to both the plasma membrane and DNA.
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spelling Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?AgingCryopreservationReactive oxygen species (ROS)Sperm freezingThe aims of this study were to evaluate cryopreserved semen of Nellore bulls of different ages and verify whether sperm quality declines with advancing age and whether lipid peroxidation and DNA damage are involved in this process. For this purpose, 40 Nellore bulls were divided into three age groups: Young, aged 1.8–2 years (n = 9); Adult, aged 3.5–7.0 years (n = 19); and Seniors, aged 8.0–14.3 years (n = 12). Three ejaculates were collected from each bull, cryopreserved and evaluated for various parameters including membrane integrity, mitochondrial potential (FITC-PSA and JC1), lipid peroxidation (C-11BODIPY 581 / 591) and oxidative DNA damage (8OHdG) using flow cytometry. The thawed semen of senior bulls was characterized by a low percentage of motile sperm (33.7 ± 6.1%), higher damage to the plasma and acrosomal membrane (37.5 ± 9.8%), and low mitochondrial potential (29.1 ± 13.8%), as well as higher percentages of peroxidated cells (53.6 ± 12.2%) and DNA damage (44.1 ± 11.0%; P < 0.05). Lipid peroxidation was negatively correlated with motility (r = −0.35, P < 0.0002), average mitochondrial potential (r = −0.42; P < 0.0001) and showed a positive correlation with membrane injury and oxidative DNA damage (r = 039; P = 0.0003). Young bulls presented superior thawed sperm quality, possibly due to greater resistance to oxidative stress and, consequently, to cryopreservation. In conclusion, the sperm quality of bull semen declines with advancing age and is strongly associated with increased oxidative damage to both the plasma membrane and DNA.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Medicine Surgery and Animal Reproduction School of Veterinary Medicine São Paulo State University – UNESPDepartment of Support Production and Animal Health School of Veterinary Medicine São Paulo State University – UNESPDepartment of Preventative Veterinary Medicine and Animal Reproduction School of Agricultural and Veterinary Sciences São Paulo State University – UNESPDepartment of Medicine Surgery and Animal Reproduction School of Veterinary Medicine São Paulo State University – UNESPDepartment of Support Production and Animal Health School of Veterinary Medicine São Paulo State University – UNESPDepartment of Preventative Veterinary Medicine and Animal Reproduction School of Agricultural and Veterinary Sciences São Paulo State University – UNESPFAPESP: 2011/16614-2FAPESP: 2011/22741-1Universidade Estadual Paulista (Unesp)Trevizan, Juliane Teramachi [UNESP]Carreira, Janaina Torres [UNESP]Carvalho, Isadora Resende [UNESP]Kipper, Bruna Helena [UNESP]Nagata, Walter Bertequine [UNESP]Perri, Silvia Helena Venturoli [UNESP]Franco Oliveira, Maria Emilia [UNESP]Pierucci, Julia Cestari [UNESP]Koivisto, Marion Burkhardt de [UNESP]2018-12-11T17:37:01Z2018-12-11T17:37:01Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8-15application/pdfhttp://dx.doi.org/10.1016/j.anireprosci.2018.04.071Animal Reproduction Science, v. 195, p. 8-15.0378-4320http://hdl.handle.net/11449/17985310.1016/j.anireprosci.2018.04.0712-s2.0-850468265302-s2.0-85046826530.pdf3725971100777730Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Science0,704info:eu-repo/semantics/openAccess2024-09-04T19:16:06Zoai:repositorio.unesp.br:11449/179853Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?
title Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?
spellingShingle Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?
Trevizan, Juliane Teramachi [UNESP]
Aging
Cryopreservation
Reactive oxygen species (ROS)
Sperm freezing
title_short Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?
title_full Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?
title_fullStr Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?
title_full_unstemmed Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?
title_sort Does lipid peroxidation and oxidative DNA damage differ in cryopreserved semen samples from young, adult and aged Nellore bulls?
author Trevizan, Juliane Teramachi [UNESP]
author_facet Trevizan, Juliane Teramachi [UNESP]
Carreira, Janaina Torres [UNESP]
Carvalho, Isadora Resende [UNESP]
Kipper, Bruna Helena [UNESP]
Nagata, Walter Bertequine [UNESP]
Perri, Silvia Helena Venturoli [UNESP]
Franco Oliveira, Maria Emilia [UNESP]
Pierucci, Julia Cestari [UNESP]
Koivisto, Marion Burkhardt de [UNESP]
author_role author
author2 Carreira, Janaina Torres [UNESP]
Carvalho, Isadora Resende [UNESP]
Kipper, Bruna Helena [UNESP]
Nagata, Walter Bertequine [UNESP]
Perri, Silvia Helena Venturoli [UNESP]
Franco Oliveira, Maria Emilia [UNESP]
Pierucci, Julia Cestari [UNESP]
Koivisto, Marion Burkhardt de [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Trevizan, Juliane Teramachi [UNESP]
Carreira, Janaina Torres [UNESP]
Carvalho, Isadora Resende [UNESP]
Kipper, Bruna Helena [UNESP]
Nagata, Walter Bertequine [UNESP]
Perri, Silvia Helena Venturoli [UNESP]
Franco Oliveira, Maria Emilia [UNESP]
Pierucci, Julia Cestari [UNESP]
Koivisto, Marion Burkhardt de [UNESP]
dc.subject.por.fl_str_mv Aging
Cryopreservation
Reactive oxygen species (ROS)
Sperm freezing
topic Aging
Cryopreservation
Reactive oxygen species (ROS)
Sperm freezing
description The aims of this study were to evaluate cryopreserved semen of Nellore bulls of different ages and verify whether sperm quality declines with advancing age and whether lipid peroxidation and DNA damage are involved in this process. For this purpose, 40 Nellore bulls were divided into three age groups: Young, aged 1.8–2 years (n = 9); Adult, aged 3.5–7.0 years (n = 19); and Seniors, aged 8.0–14.3 years (n = 12). Three ejaculates were collected from each bull, cryopreserved and evaluated for various parameters including membrane integrity, mitochondrial potential (FITC-PSA and JC1), lipid peroxidation (C-11BODIPY 581 / 591) and oxidative DNA damage (8OHdG) using flow cytometry. The thawed semen of senior bulls was characterized by a low percentage of motile sperm (33.7 ± 6.1%), higher damage to the plasma and acrosomal membrane (37.5 ± 9.8%), and low mitochondrial potential (29.1 ± 13.8%), as well as higher percentages of peroxidated cells (53.6 ± 12.2%) and DNA damage (44.1 ± 11.0%; P < 0.05). Lipid peroxidation was negatively correlated with motility (r = −0.35, P < 0.0002), average mitochondrial potential (r = −0.42; P < 0.0001) and showed a positive correlation with membrane injury and oxidative DNA damage (r = 039; P = 0.0003). Young bulls presented superior thawed sperm quality, possibly due to greater resistance to oxidative stress and, consequently, to cryopreservation. In conclusion, the sperm quality of bull semen declines with advancing age and is strongly associated with increased oxidative damage to both the plasma membrane and DNA.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:37:01Z
2018-12-11T17:37:01Z
2018-08-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.anireprosci.2018.04.071
Animal Reproduction Science, v. 195, p. 8-15.
0378-4320
http://hdl.handle.net/11449/179853
10.1016/j.anireprosci.2018.04.071
2-s2.0-85046826530
2-s2.0-85046826530.pdf
3725971100777730
url http://dx.doi.org/10.1016/j.anireprosci.2018.04.071
http://hdl.handle.net/11449/179853
identifier_str_mv Animal Reproduction Science, v. 195, p. 8-15.
0378-4320
10.1016/j.anireprosci.2018.04.071
2-s2.0-85046826530
2-s2.0-85046826530.pdf
3725971100777730
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Animal Reproduction Science
0,704
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8-15
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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