Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase

Detalhes bibliográficos
Autor(a) principal: Bonifacio, Aurenivia
Data de Publicação: 2016
Outros Autores: Carvalho, Fabrício E.L., Martins, Marcio O., Lima Neto, Milton C. [UNESP], Cunha, Juliana R., Ribeiro, Carolina W., Margis-Pinheiro, Marcia, Silveira, Joaquim A.G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jplph.2016.06.015
http://hdl.handle.net/11449/173161
Resumo: The maintenance of H2O2 homeostasis and signaling mechanisms in plant subcellular compartments is greatly dependent on cytosolic ascorbate peroxidases (APX1 and APX2) and peroxisomal catalase (CAT) activities. APX1/2 knockdown plants were utilized in this study to clarify the role of increased cytosolic H2O2 levels as a signal to trigger the antioxidant defense system against oxidative stress generated in peroxisomes after 3-aminotriazole-inhibited catalase (CAT). Before supplying 3-AT, silenced APX1/2 plants showed marked changes in their oxidative and antioxidant profiles in comparison to NT plants. After supplying 3-AT, APX1/2 plants triggered up-expression of genes belonging to APX (OsAPX7 and OsAPX8) and GPX families (OsGPX1, OsGPX2, OsGPX3 and OsGPX5), but to a lower extent than in NT plants. In addition, APX1/2 exhibited lower glycolate oxidase (GO) activity, higher CO2 assimilation, higher cellular integrity and higher oxidation of GSH, whereas the H2O2 and lipid peroxidation levels remained unchanged. This evidence indicates that redox pre-acclimation displayed by silenced rice contributed to coping with oxidative stress generated by 3-AT. We suggest that APX1/2 plants were able to trigger alternative oxidative and antioxidant mechanisms involving signaling by H2O2, allowing these plants to display effective physiological responses for protection against oxidative damage generated by 3-AT, compared to non-transformed plants.
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spelling Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalaseAscorbate peroxidaseHydrogen peroxideOryza sativaPhotorespirationPhotosynthesisRedox metabolismThe maintenance of H2O2 homeostasis and signaling mechanisms in plant subcellular compartments is greatly dependent on cytosolic ascorbate peroxidases (APX1 and APX2) and peroxisomal catalase (CAT) activities. APX1/2 knockdown plants were utilized in this study to clarify the role of increased cytosolic H2O2 levels as a signal to trigger the antioxidant defense system against oxidative stress generated in peroxisomes after 3-aminotriazole-inhibited catalase (CAT). Before supplying 3-AT, silenced APX1/2 plants showed marked changes in their oxidative and antioxidant profiles in comparison to NT plants. After supplying 3-AT, APX1/2 plants triggered up-expression of genes belonging to APX (OsAPX7 and OsAPX8) and GPX families (OsGPX1, OsGPX2, OsGPX3 and OsGPX5), but to a lower extent than in NT plants. In addition, APX1/2 exhibited lower glycolate oxidase (GO) activity, higher CO2 assimilation, higher cellular integrity and higher oxidation of GSH, whereas the H2O2 and lipid peroxidation levels remained unchanged. This evidence indicates that redox pre-acclimation displayed by silenced rice contributed to coping with oxidative stress generated by 3-AT. We suggest that APX1/2 plants were able to trigger alternative oxidative and antioxidant mechanisms involving signaling by H2O2, allowing these plants to display effective physiological responses for protection against oxidative damage generated by 3-AT, compared to non-transformed plants.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Biochemistry and Molecular Biology Federal University of Ceara Fortaleza/CEBiosciences Institute São Paulo State University UNESP Coastal Campus, São Vicente/SP, P.O. Box 73601Department of Genetics Federal University of Rio Grande do SulBiosciences Institute São Paulo State University UNESP Coastal Campus, São Vicente/SP, P.O. Box 73601CNPq: 486231-2012-7Fortaleza/CEUniversidade Estadual Paulista (Unesp)Federal University of Rio Grande do SulBonifacio, AureniviaCarvalho, Fabrício E.L.Martins, Marcio O.Lima Neto, Milton C. [UNESP]Cunha, Juliana R.Ribeiro, Carolina W.Margis-Pinheiro, MarciaSilveira, Joaquim A.G.2018-12-11T17:03:55Z2018-12-11T17:03:55Z2016-08-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-27application/pdfhttp://dx.doi.org/10.1016/j.jplph.2016.06.015Journal of Plant Physiology, v. 201, p. 17-27.0176-1617http://hdl.handle.net/11449/17316110.1016/j.jplph.2016.06.0152-s2.0-849768928092-s2.0-84976892809.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Plant Physiology1,178info:eu-repo/semantics/openAccess2024-01-27T06:52:32Zoai:repositorio.unesp.br:11449/173161Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:03:41.838216Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase
title Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase
spellingShingle Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase
Bonifacio, Aurenivia
Ascorbate peroxidase
Hydrogen peroxide
Oryza sativa
Photorespiration
Photosynthesis
Redox metabolism
title_short Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase
title_full Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase
title_fullStr Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase
title_full_unstemmed Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase
title_sort Silenced rice in both cytosolic ascorbate peroxidases displays pre-acclimation to cope with oxidative stress induced by 3-aminotriazole-inhibited catalase
author Bonifacio, Aurenivia
author_facet Bonifacio, Aurenivia
Carvalho, Fabrício E.L.
Martins, Marcio O.
Lima Neto, Milton C. [UNESP]
Cunha, Juliana R.
Ribeiro, Carolina W.
Margis-Pinheiro, Marcia
Silveira, Joaquim A.G.
author_role author
author2 Carvalho, Fabrício E.L.
Martins, Marcio O.
Lima Neto, Milton C. [UNESP]
Cunha, Juliana R.
Ribeiro, Carolina W.
Margis-Pinheiro, Marcia
Silveira, Joaquim A.G.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fortaleza/CE
Universidade Estadual Paulista (Unesp)
Federal University of Rio Grande do Sul
dc.contributor.author.fl_str_mv Bonifacio, Aurenivia
Carvalho, Fabrício E.L.
Martins, Marcio O.
Lima Neto, Milton C. [UNESP]
Cunha, Juliana R.
Ribeiro, Carolina W.
Margis-Pinheiro, Marcia
Silveira, Joaquim A.G.
dc.subject.por.fl_str_mv Ascorbate peroxidase
Hydrogen peroxide
Oryza sativa
Photorespiration
Photosynthesis
Redox metabolism
topic Ascorbate peroxidase
Hydrogen peroxide
Oryza sativa
Photorespiration
Photosynthesis
Redox metabolism
description The maintenance of H2O2 homeostasis and signaling mechanisms in plant subcellular compartments is greatly dependent on cytosolic ascorbate peroxidases (APX1 and APX2) and peroxisomal catalase (CAT) activities. APX1/2 knockdown plants were utilized in this study to clarify the role of increased cytosolic H2O2 levels as a signal to trigger the antioxidant defense system against oxidative stress generated in peroxisomes after 3-aminotriazole-inhibited catalase (CAT). Before supplying 3-AT, silenced APX1/2 plants showed marked changes in their oxidative and antioxidant profiles in comparison to NT plants. After supplying 3-AT, APX1/2 plants triggered up-expression of genes belonging to APX (OsAPX7 and OsAPX8) and GPX families (OsGPX1, OsGPX2, OsGPX3 and OsGPX5), but to a lower extent than in NT plants. In addition, APX1/2 exhibited lower glycolate oxidase (GO) activity, higher CO2 assimilation, higher cellular integrity and higher oxidation of GSH, whereas the H2O2 and lipid peroxidation levels remained unchanged. This evidence indicates that redox pre-acclimation displayed by silenced rice contributed to coping with oxidative stress generated by 3-AT. We suggest that APX1/2 plants were able to trigger alternative oxidative and antioxidant mechanisms involving signaling by H2O2, allowing these plants to display effective physiological responses for protection against oxidative damage generated by 3-AT, compared to non-transformed plants.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-20
2018-12-11T17:03:55Z
2018-12-11T17:03:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jplph.2016.06.015
Journal of Plant Physiology, v. 201, p. 17-27.
0176-1617
http://hdl.handle.net/11449/173161
10.1016/j.jplph.2016.06.015
2-s2.0-84976892809
2-s2.0-84976892809.pdf
url http://dx.doi.org/10.1016/j.jplph.2016.06.015
http://hdl.handle.net/11449/173161
identifier_str_mv Journal of Plant Physiology, v. 201, p. 17-27.
0176-1617
10.1016/j.jplph.2016.06.015
2-s2.0-84976892809
2-s2.0-84976892809.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Plant Physiology
1,178
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17-27
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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