Quick preparative separation of natural naphthopyranones with antioxidant activity by high-speed counter-current chromatography

• Autor(a) principal: Leitão, Gilda G.
• Data de Publicação: 2002
• Outros Autores: Leitão, Suzana G., Vilegas, Wagner [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.znaturforsch.com/ac/v57c/s57c1051.pdf; http://hdl.handle.net/11449/67016
• topic: Antioxidant Activity; High-Speed Counter-Current Chromatography; Naphthopyranone Glycosides; Antioxidants; Brazil; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Countercurrent Distribution; Eriocaulaceae; Ethanol; Free Radicals; Glycosides; Heterocyclic Compounds, 3-Ring; Naphthalenes; Plant Extracts; Pyrones; Paepalanthus

The natural naphthopyranones paepalantine (1), paepalantine-9O-β-D-glucopyranoside (2) and paepalantine-9-O-β-D-allopyranosyl-(1→6)-O-β-D-glucopyranoside (3) were separated in a preparative scale from the ethanolic extract of the capitula of Paepalanthus bromelioides by high-speed counter-current chromatography (HSCCC). The solvent system used was composed of water-ethanol-ethyl acetate-hexane (10:4:10:4, v/v/v/v). This technique led to the separation of the three different naphthopyranone glycosides in pure form in approximately 7 hours. Paepalantine showed a good antioxidant activity when assayed by the DPPH radical spectrophotometric assay.