Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/s0100-736x2015001000002 http://hdl.handle.net/11449/172293 |
Resumo: | Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent- culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non -injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis. |
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Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitisCattlePeriodontitisPorphyromonas spp.Prevotella spp.Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent- culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non -injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis.Programa de Pós-Graduação em Medicina Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Patologia e Propedêutica Clínica Faculdade de Odontologia de Araçatuba Unesp, Rua José Bonifácio 1193Departamento de Matemática Faculdade de Engenharia de Ilha Solteira Unesp, Alameda Rio de Janeiro 266Ex-Pesquisador da Empresa Brasileira de Pesquisa Agropecuária (Embrapa) General Editor of 'Pesquisa Veterinária BrasileiraDepartamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba Unesp Campus de Araçatuba, Rua Clóvis Pestana 793Programa de Pós-Graduação em Medicina Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp), Via de Acesso Professor Paulo Donato Castellane s/nDepartamento de Patologia e Propedêutica Clínica Faculdade de Odontologia de Araçatuba Unesp, Rua José Bonifácio 1193Departamento de Matemática Faculdade de Engenharia de Ilha Solteira Unesp, Alameda Rio de Janeiro 266Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba Unesp Campus de Araçatuba, Rua Clóvis Pestana 793Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Borsanelli, Ana Carolina [UNESP]Júnior, Elerson Gaetti-Jardim [UNESP]Schweitzer, Christiane Marie [UNESP]Döbereiner, JürgenDutra, Iveraldo S. [UNESP]2018-12-11T16:59:34Z2018-12-11T16:59:34Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article829-834application/pdfhttp://dx.doi.org/10.1590/s0100-736x2015001000002Pesquisa Veterinaria Brasileira, v. 35, n. 10, p. 829-834, 2015.1678-51500100-736Xhttp://hdl.handle.net/11449/17229310.1590/s0100-736x2015001000002S0100-736X20150010008292-s2.0-84949745362S0100-736X2015001000829.pdf00011553552103270000-0001-9561-8281Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-09-04T19:16:06Zoai:repositorio.unesp.br:11449/172293Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:16:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis |
title |
Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis |
spellingShingle |
Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis Borsanelli, Ana Carolina [UNESP] Cattle Periodontitis Porphyromonas spp. Prevotella spp. |
title_short |
Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis |
title_full |
Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis |
title_fullStr |
Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis |
title_full_unstemmed |
Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis |
title_sort |
Presence of Porphyromonas and Prevotella species in the oral microflora of cattle with periodontitis |
author |
Borsanelli, Ana Carolina [UNESP] |
author_facet |
Borsanelli, Ana Carolina [UNESP] Júnior, Elerson Gaetti-Jardim [UNESP] Schweitzer, Christiane Marie [UNESP] Döbereiner, Jürgen Dutra, Iveraldo S. [UNESP] |
author_role |
author |
author2 |
Júnior, Elerson Gaetti-Jardim [UNESP] Schweitzer, Christiane Marie [UNESP] Döbereiner, Jürgen Dutra, Iveraldo S. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Borsanelli, Ana Carolina [UNESP] Júnior, Elerson Gaetti-Jardim [UNESP] Schweitzer, Christiane Marie [UNESP] Döbereiner, Jürgen Dutra, Iveraldo S. [UNESP] |
dc.subject.por.fl_str_mv |
Cattle Periodontitis Porphyromonas spp. Prevotella spp. |
topic |
Cattle Periodontitis Porphyromonas spp. Prevotella spp. |
description |
Bovine periodontitis is a progressive purulent infectious process associated with the presence of strictly and facultative anaerobic subgingival biofilm and epidemiologically related to soil management in large geographic areas of Brazil. This study aimed to detect species of the genera Porphyromonas and Prevotella, which occurr in periodontal pockets of cattle with lesions deeper than 5mm (n=26) and in gingival sulcus of animals considered periodontally healthy (n=25). Presence of the microorganisms was evaluated by independent- culture medium diagnostic method, using polymerase chain reaction (PCR) with specific primers of Porphyromonas asaccharolytica, P. endodontalis, P. gingivalis, P. gulae, Prevotella buccae, P. intermedia, P. loescheii, P. melaninogenica, P. nigrescens, P. oralis and P. tannerae. The species P. endodontalis (80.7%), P. melaninogenica (73.1%) and P. intermedia (61.5%) were the most predominant in samples of cattle with periodontitis. Regarding non -injured gingival sulcus of cattle, P. endodontalis (40%) and P. loeschei (40%) prevailed. Porphyromonas gingivalis, P. gulae and Prevotella tannerae were not detected in the 51 samples studied. Data evaluation by T test, enabled to verify that ocorrence of Porphyromonas asaccharolytica (p=0.000003), P. endodontalis (p=0.0023), Prevotella buccae (p=0.0017), P. intermedia (p=0.0020), P. melaninogenica (p=0.00006) and P. oralis (p=0.0028) is correlated with bovine periodontitis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-12-11T16:59:34Z 2018-12-11T16:59:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/s0100-736x2015001000002 Pesquisa Veterinaria Brasileira, v. 35, n. 10, p. 829-834, 2015. 1678-5150 0100-736X http://hdl.handle.net/11449/172293 10.1590/s0100-736x2015001000002 S0100-736X2015001000829 2-s2.0-84949745362 S0100-736X2015001000829.pdf 0001155355210327 0000-0001-9561-8281 |
url |
http://dx.doi.org/10.1590/s0100-736x2015001000002 http://hdl.handle.net/11449/172293 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 35, n. 10, p. 829-834, 2015. 1678-5150 0100-736X 10.1590/s0100-736x2015001000002 S0100-736X2015001000829 2-s2.0-84949745362 S0100-736X2015001000829.pdf 0001155355210327 0000-0001-9561-8281 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
829-834 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021414172884992 |