Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2016/3521413 http://hdl.handle.net/11449/173934 |
Resumo: | This study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 107 UFC/mL of K. pneumoniae was distributed into 96-well microplates (n=10) and after 48 h at 37°C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical significant reduction (p<0.05) of planktonic form and biofilm. P paniculata K., R. officinalis L., and J. regia L. showed reductions in biomass of 55.6, 58.1, and 18.65% and cell viability reduction of 72.4, 65.1, and 31.5%, respectively. The reduction obtained with P. paniculata and R. officinalis extracts was similar to the reduction obtained with chlorhexidine digluconate 2%. In conclusion, all extracts have microbicidal action on the planktonic form but only P. paniculata K. and R. officinalis L. were effective against biofilm. |
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Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro studyThis study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 107 UFC/mL of K. pneumoniae was distributed into 96-well microplates (n=10) and after 48 h at 37°C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical significant reduction (p<0.05) of planktonic form and biofilm. P paniculata K., R. officinalis L., and J. regia L. showed reductions in biomass of 55.6, 58.1, and 18.65% and cell viability reduction of 72.4, 65.1, and 31.5%, respectively. The reduction obtained with P. paniculata and R. officinalis extracts was similar to the reduction obtained with chlorhexidine digluconate 2%. In conclusion, all extracts have microbicidal action on the planktonic form but only P. paniculata K. and R. officinalis L. were effective against biofilm.Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology São José dos Campos, Francisco José Longo 777Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology São José dos Campos, Francisco José Longo 777Universidade Estadual Paulista (Unesp)De Paula Ramos, Lucas [UNESP]Da Rocha Santos, Carlos Eduardo [UNESP]Camargo Reis Mello, Daphne [UNESP]Nishiama Theodoro, Lígia [UNESP]De Oliveira, Felipe Eduardo [UNESP]Back Brito, Graziella N. [UNESP]Campos Junqueira, Juliana [UNESP]Cardoso Jorge, Antonio Olavo [UNESP]Dias De Oliveira, Luciane [UNESP]2018-12-11T17:08:24Z2018-12-11T17:08:24Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2016/3521413Scientific World Journal, v. 2016.1537-744X2356-6140http://hdl.handle.net/11449/17393410.1155/2016/35214132-s2.0-850062378602-s2.0-85006237860.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific World Journalinfo:eu-repo/semantics/openAccess2024-01-02T06:18:39Zoai:repositorio.unesp.br:11449/173934Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:55:31.923164Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study |
title |
Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study |
spellingShingle |
Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study De Paula Ramos, Lucas [UNESP] |
title_short |
Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study |
title_full |
Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study |
title_fullStr |
Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study |
title_full_unstemmed |
Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study |
title_sort |
Klebsiella pneumoniae planktonic and biofilm reduction by different plant extracts: In vitro study |
author |
De Paula Ramos, Lucas [UNESP] |
author_facet |
De Paula Ramos, Lucas [UNESP] Da Rocha Santos, Carlos Eduardo [UNESP] Camargo Reis Mello, Daphne [UNESP] Nishiama Theodoro, Lígia [UNESP] De Oliveira, Felipe Eduardo [UNESP] Back Brito, Graziella N. [UNESP] Campos Junqueira, Juliana [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] Dias De Oliveira, Luciane [UNESP] |
author_role |
author |
author2 |
Da Rocha Santos, Carlos Eduardo [UNESP] Camargo Reis Mello, Daphne [UNESP] Nishiama Theodoro, Lígia [UNESP] De Oliveira, Felipe Eduardo [UNESP] Back Brito, Graziella N. [UNESP] Campos Junqueira, Juliana [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] Dias De Oliveira, Luciane [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
De Paula Ramos, Lucas [UNESP] Da Rocha Santos, Carlos Eduardo [UNESP] Camargo Reis Mello, Daphne [UNESP] Nishiama Theodoro, Lígia [UNESP] De Oliveira, Felipe Eduardo [UNESP] Back Brito, Graziella N. [UNESP] Campos Junqueira, Juliana [UNESP] Cardoso Jorge, Antonio Olavo [UNESP] Dias De Oliveira, Luciane [UNESP] |
description |
This study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 107 UFC/mL of K. pneumoniae was distributed into 96-well microplates (n=10) and after 48 h at 37°C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical significant reduction (p<0.05) of planktonic form and biofilm. P paniculata K., R. officinalis L., and J. regia L. showed reductions in biomass of 55.6, 58.1, and 18.65% and cell viability reduction of 72.4, 65.1, and 31.5%, respectively. The reduction obtained with P. paniculata and R. officinalis extracts was similar to the reduction obtained with chlorhexidine digluconate 2%. In conclusion, all extracts have microbicidal action on the planktonic form but only P. paniculata K. and R. officinalis L. were effective against biofilm. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:08:24Z 2018-12-11T17:08:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2016/3521413 Scientific World Journal, v. 2016. 1537-744X 2356-6140 http://hdl.handle.net/11449/173934 10.1155/2016/3521413 2-s2.0-85006237860 2-s2.0-85006237860.pdf |
url |
http://dx.doi.org/10.1155/2016/3521413 http://hdl.handle.net/11449/173934 |
identifier_str_mv |
Scientific World Journal, v. 2016. 1537-744X 2356-6140 10.1155/2016/3521413 2-s2.0-85006237860 2-s2.0-85006237860.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific World Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129373698523136 |