ZIKV B-cell epitopes for immunodiagnostic tests
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1016/j.jim.2022.113246 http://hdl.handle.net/11449/223639 |
Resumo: | The use of serological tests is valuable to diagnose Zika virus (ZIKV) infection and carry out epidemiological surveillance. However, ZIKV serological tests may result in false positives due to cross-reactivity between antibodies against other Flavivirus, especially dengue virus that worldwide disseminated. We used three online tools to predict amino acid sequences of B-cell epitopes. We selected and synthetized two epitopes that showed appropriate features in the molecular dynamic simulation and demonstrated to be suitable for serological assays. |
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ZIKV B-cell epitopes for immunodiagnostic testsArbovirusBioinformaticsImmunoassayPeptideThe use of serological tests is valuable to diagnose Zika virus (ZIKV) infection and carry out epidemiological surveillance. However, ZIKV serological tests may result in false positives due to cross-reactivity between antibodies against other Flavivirus, especially dengue virus that worldwide disseminated. We used three online tools to predict amino acid sequences of B-cell epitopes. We selected and synthetized two epitopes that showed appropriate features in the molecular dynamic simulation and demonstrated to be suitable for serological assays.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Mato GrossoUniversidade Federal de Mato Grosso (UFMT) Instituto de Ciências da Saúde Avenida Alexandre Ferronato, 1200, SinopFaculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, Vila São Pedro, São José do Rio PretoFundação Oswaldo Cruz-PE/Centro de Pesquisas Aggeu Magalhães, Avenida Prof. Moraes Rego, Cidade Universitária, PernambucoUniversidade do Estado de Mato Grosso Departamento de Biologia, Rua Prof. Dr. Renato Figueiro Varella, Nova XavantinaCAPES: 001Fundação de Amparo à Pesquisa do Estado de Mato Grosso: 0499331/2016Avenida Alexandre FerronatoFaculdade de Medicina de São José do Rio PretoFundação Oswaldo Cruz-PE/Centro de Pesquisas Aggeu MagalhãesUniversidade Estadual Paulista (UNESP)Universidade do Estado de Mato GrossoKubiszeski, Janaina Rigottida Silva, David José FerreiraMenezes, Gabriela de Limada Silva, Roosevelt AlvesZini, NathaliaNogueira, Maurício LacerdaPena, Lindomar JoséPiccoli, Julia PintoCilli, Eduardo Maffudda Silva, Joaquim ManoelBronzoni, Roberta Vieira de Morais2022-04-28T19:51:55Z2022-04-28T19:51:55Z2022-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jim.2022.113246Journal of Immunological Methods, v. 504.1872-79050022-1759http://hdl.handle.net/11449/22363910.1016/j.jim.2022.1132462-s2.0-85126303568Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Immunological Methodsinfo:eu-repo/semantics/openAccess2022-04-28T19:51:55Zoai:repositorio.unesp.br:11449/223639Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:51:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
ZIKV B-cell epitopes for immunodiagnostic tests |
| title |
ZIKV B-cell epitopes for immunodiagnostic tests |
| spellingShingle |
ZIKV B-cell epitopes for immunodiagnostic tests Kubiszeski, Janaina Rigotti Arbovirus Bioinformatics Immunoassay Peptide |
| title_short |
ZIKV B-cell epitopes for immunodiagnostic tests |
| title_full |
ZIKV B-cell epitopes for immunodiagnostic tests |
| title_fullStr |
ZIKV B-cell epitopes for immunodiagnostic tests |
| title_full_unstemmed |
ZIKV B-cell epitopes for immunodiagnostic tests |
| title_sort |
ZIKV B-cell epitopes for immunodiagnostic tests |
| author |
Kubiszeski, Janaina Rigotti |
| author_facet |
Kubiszeski, Janaina Rigotti da Silva, David José Ferreira Menezes, Gabriela de Lima da Silva, Roosevelt Alves Zini, Nathalia Nogueira, Maurício Lacerda Pena, Lindomar José Piccoli, Julia Pinto Cilli, Eduardo Maffud da Silva, Joaquim Manoel Bronzoni, Roberta Vieira de Morais |
| author_role |
author |
| author2 |
da Silva, David José Ferreira Menezes, Gabriela de Lima da Silva, Roosevelt Alves Zini, Nathalia Nogueira, Maurício Lacerda Pena, Lindomar José Piccoli, Julia Pinto Cilli, Eduardo Maffud da Silva, Joaquim Manoel Bronzoni, Roberta Vieira de Morais |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Avenida Alexandre Ferronato Faculdade de Medicina de São José do Rio Preto Fundação Oswaldo Cruz-PE/Centro de Pesquisas Aggeu Magalhães Universidade Estadual Paulista (UNESP) Universidade do Estado de Mato Grosso |
| dc.contributor.author.fl_str_mv |
Kubiszeski, Janaina Rigotti da Silva, David José Ferreira Menezes, Gabriela de Lima da Silva, Roosevelt Alves Zini, Nathalia Nogueira, Maurício Lacerda Pena, Lindomar José Piccoli, Julia Pinto Cilli, Eduardo Maffud da Silva, Joaquim Manoel Bronzoni, Roberta Vieira de Morais |
| dc.subject.por.fl_str_mv |
Arbovirus Bioinformatics Immunoassay Peptide |
| topic |
Arbovirus Bioinformatics Immunoassay Peptide |
| description |
The use of serological tests is valuable to diagnose Zika virus (ZIKV) infection and carry out epidemiological surveillance. However, ZIKV serological tests may result in false positives due to cross-reactivity between antibodies against other Flavivirus, especially dengue virus that worldwide disseminated. We used three online tools to predict amino acid sequences of B-cell epitopes. We selected and synthetized two epitopes that showed appropriate features in the molecular dynamic simulation and demonstrated to be suitable for serological assays. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-04-28T19:51:55Z 2022-04-28T19:51:55Z 2022-05-01 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jim.2022.113246 Journal of Immunological Methods, v. 504. 1872-7905 0022-1759 http://hdl.handle.net/11449/223639 10.1016/j.jim.2022.113246 2-s2.0-85126303568 |
| url |
http://dx.doi.org/10.1016/j.jim.2022.113246 http://hdl.handle.net/11449/223639 |
| identifier_str_mv |
Journal of Immunological Methods, v. 504. 1872-7905 0022-1759 10.1016/j.jim.2022.113246 2-s2.0-85126303568 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Journal of Immunological Methods |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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|
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1803046735674605568 |