Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites

Detalhes bibliográficos
Autor(a) principal: Valerio-Bolas, Ana
Data de Publicação: 2019
Outros Autores: Pereira, Maria, Alexandre-Pires, Graca, Santos-Mateus, David, Rodrigues, Armanda, Rafael-Fernandes, Mariana, Gabriel, Aurea, Passero, Felipe [UNESP], Santos-Gomes, Gabriela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.cellimm.2018.11.003
http://hdl.handle.net/11449/185400
Resumo: Neutrophils are short-lived phagocytic cells equipped with several receptors for pathogen recognition and phagocytosis and have intracellular and extracellular effector mechanisms that can inactivate pathogens. Leishmaniases are diseases caused by different species of Leishmania that mainly afflicts poorer populations of tropical and subtropical regions and immunocompromised individuals. Thus, the present study aims to investigate the effector response of murine neutrophils to species of Leishmania causing American cutaneous leishmaniasis and zoonotic visceral leishmaniasis by evaluating pattern recognition receptors (PRR) and intracellular and extracellular effector microbicide activity. When exposed to Leishmania parasites, mouse neutrophils produced superoxide, released enzymes in the extracellular space and generated neutrophil extracellular traps, although PRR gene expression is negatively regulated. L. infantum, L. guyanensis, and L. shawi inhibited enzymatic activity, whereas L. amazonensis reduced the emission of extracellular structures. These findings indicate that although neutrophils trigger several microbicide mechanisms, Leishmania parasites can manipulate extracellular effector mechanisms. The present study also provides evidence that neutrophils can internalize parasites by coiling phagocytosis.
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spelling Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasitesInnate immunityMouse neutrophilsPattern recognition receptorsPhagocytosisOxidative burstDegranulationNeutrophil extracellular trapsLeishmania spp.Neutrophils are short-lived phagocytic cells equipped with several receptors for pathogen recognition and phagocytosis and have intracellular and extracellular effector mechanisms that can inactivate pathogens. Leishmaniases are diseases caused by different species of Leishmania that mainly afflicts poorer populations of tropical and subtropical regions and immunocompromised individuals. Thus, the present study aims to investigate the effector response of murine neutrophils to species of Leishmania causing American cutaneous leishmaniasis and zoonotic visceral leishmaniasis by evaluating pattern recognition receptors (PRR) and intracellular and extracellular effector microbicide activity. When exposed to Leishmania parasites, mouse neutrophils produced superoxide, released enzymes in the extracellular space and generated neutrophil extracellular traps, although PRR gene expression is negatively regulated. L. infantum, L. guyanensis, and L. shawi inhibited enzymatic activity, whereas L. amazonensis reduced the emission of extracellular structures. These findings indicate that although neutrophils trigger several microbicide mechanisms, Leishmania parasites can manipulate extracellular effector mechanisms. The present study also provides evidence that neutrophils can internalize parasites by coiling phagocytosis.Portuguese Foundation for Science and Technology (FCT)Univ Nova Lisboa, IHMT, GHTM, Rua Junqueira 100, P-1349008 Lisbon, PortugalEscola Super Agr Elvas, Edificio Trem Auto, P-7350903 Elvas, PortugalUniv Lisbon, Fac Med Vet, Ctr Invest Interdisciplinar Sanidade Anim, Av Univ Tecn, P-1300477 Lisbon, PortugalUniv Estadual Paulista, Sao Vicente Unit, Biosci Inst, Paulista Coast Campus, BR-11330900 Sao Vicente, SP, BrazilUniv Estadual Paulista, Sao Vicente Unit, Biosci Inst, Paulista Coast Campus, BR-11330900 Sao Vicente, SP, BrazilPortuguese Foundation for Science and Technology (FCT): PTDC/CVT/113121/2009Portuguese Foundation for Science and Technology (FCT): GHTM - UID/Multi/04413/2013Portuguese Foundation for Science and Technology (FCT): CIISA - UID/CVT/267/2013Elsevier B.V.Univ Nova LisboaEscola Super Agr ElvasUniv LisbonUniversidade Estadual Paulista (Unesp)Valerio-Bolas, AnaPereira, MariaAlexandre-Pires, GracaSantos-Mateus, DavidRodrigues, ArmandaRafael-Fernandes, MarianaGabriel, AureaPassero, Felipe [UNESP]Santos-Gomes, Gabriela2019-10-04T12:35:12Z2019-10-04T12:35:12Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article76-84http://dx.doi.org/10.1016/j.cellimm.2018.11.003Cellular Immunology. San Diego: Academic Press Inc Elsevier Science, v. 335, p. 76-84, 2019.0008-8749http://hdl.handle.net/11449/18540010.1016/j.cellimm.2018.11.003WOS:000457737400010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Immunologyinfo:eu-repo/semantics/openAccess2021-10-23T14:26:50Zoai:repositorio.unesp.br:11449/185400Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:47:57.991292Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites
title Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites
spellingShingle Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites
Valerio-Bolas, Ana
Innate immunity
Mouse neutrophils
Pattern recognition receptors
Phagocytosis
Oxidative burst
Degranulation
Neutrophil extracellular traps
Leishmania spp.
title_short Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites
title_full Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites
title_fullStr Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites
title_full_unstemmed Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites
title_sort Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites
author Valerio-Bolas, Ana
author_facet Valerio-Bolas, Ana
Pereira, Maria
Alexandre-Pires, Graca
Santos-Mateus, David
Rodrigues, Armanda
Rafael-Fernandes, Mariana
Gabriel, Aurea
Passero, Felipe [UNESP]
Santos-Gomes, Gabriela
author_role author
author2 Pereira, Maria
Alexandre-Pires, Graca
Santos-Mateus, David
Rodrigues, Armanda
Rafael-Fernandes, Mariana
Gabriel, Aurea
Passero, Felipe [UNESP]
Santos-Gomes, Gabriela
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Nova Lisboa
Escola Super Agr Elvas
Univ Lisbon
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Valerio-Bolas, Ana
Pereira, Maria
Alexandre-Pires, Graca
Santos-Mateus, David
Rodrigues, Armanda
Rafael-Fernandes, Mariana
Gabriel, Aurea
Passero, Felipe [UNESP]
Santos-Gomes, Gabriela
dc.subject.por.fl_str_mv Innate immunity
Mouse neutrophils
Pattern recognition receptors
Phagocytosis
Oxidative burst
Degranulation
Neutrophil extracellular traps
Leishmania spp.
topic Innate immunity
Mouse neutrophils
Pattern recognition receptors
Phagocytosis
Oxidative burst
Degranulation
Neutrophil extracellular traps
Leishmania spp.
description Neutrophils are short-lived phagocytic cells equipped with several receptors for pathogen recognition and phagocytosis and have intracellular and extracellular effector mechanisms that can inactivate pathogens. Leishmaniases are diseases caused by different species of Leishmania that mainly afflicts poorer populations of tropical and subtropical regions and immunocompromised individuals. Thus, the present study aims to investigate the effector response of murine neutrophils to species of Leishmania causing American cutaneous leishmaniasis and zoonotic visceral leishmaniasis by evaluating pattern recognition receptors (PRR) and intracellular and extracellular effector microbicide activity. When exposed to Leishmania parasites, mouse neutrophils produced superoxide, released enzymes in the extracellular space and generated neutrophil extracellular traps, although PRR gene expression is negatively regulated. L. infantum, L. guyanensis, and L. shawi inhibited enzymatic activity, whereas L. amazonensis reduced the emission of extracellular structures. These findings indicate that although neutrophils trigger several microbicide mechanisms, Leishmania parasites can manipulate extracellular effector mechanisms. The present study also provides evidence that neutrophils can internalize parasites by coiling phagocytosis.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-04T12:35:12Z
2019-10-04T12:35:12Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.cellimm.2018.11.003
Cellular Immunology. San Diego: Academic Press Inc Elsevier Science, v. 335, p. 76-84, 2019.
0008-8749
http://hdl.handle.net/11449/185400
10.1016/j.cellimm.2018.11.003
WOS:000457737400010
url http://dx.doi.org/10.1016/j.cellimm.2018.11.003
http://hdl.handle.net/11449/185400
identifier_str_mv Cellular Immunology. San Diego: Academic Press Inc Elsevier Science, v. 335, p. 76-84, 2019.
0008-8749
10.1016/j.cellimm.2018.11.003
WOS:000457737400010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Immunology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 76-84
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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