Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anaerobe.2018.05.015 http://hdl.handle.net/11449/176538 |
Resumo: | Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are strongly associated with periodontitis, and their evaluations are relevant to understand their role in the etiology and progression of periodontal diseases. In this study, the qualitative and quantitative detection of A. actinomycetemcomitans and F. nucleatum, as well as their genetic diversity, were evaluated in individuals with gingivitis, chronic periodontitis and periodontally healthy. In addition, the biotyping, serotyping, and prevalence of the ltx and cdt genes in A. actinomycetemcomitans were also determined. Subgingival biofilms obtained from gingivitis (70), periodontitis (75) and healthy (95) individuals were analyzed by cultures and PCR. Bacterial typing and presence of ltx and cdt genes in A. actinomycetemcomitans were also verified. DNA from A. actinomycetemcomitans and F. nucleatum was detected respectively, in 65.7% and 57.1% of gingivitis, 80% and 68% of periodontitis, and 57.8% and 37.8% of healthy. A. actinomycetemcomitans from gingivitis were biotypes I, II, IV, V, and X, and serotypes a, c, and e. In periodontitis, biotypes II, VI, and X, and serotypes a, b, and c were found. In healthy subjects, biotypes II and X, and serotypes b and c were found. The LTX and ltxA were observed in strains from gingivitis and periodontitis pockets. Subsequently, our data also showed no direct relationship between ltxA gene expression and leukotoxin gene 530-bp presence. On the other hand, cdt gene predominated during the inflammatory disease process. Our results strongly support a role of A. actinomycetemcomitans and F. nucleatum in advanced stage of periodontal disease. |
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Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditionsAggregatibacter actinomycetemcomitansBacterial typingFusobacterium nucleatumOral microbiologyPeriodontal diseaseAggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are strongly associated with periodontitis, and their evaluations are relevant to understand their role in the etiology and progression of periodontal diseases. In this study, the qualitative and quantitative detection of A. actinomycetemcomitans and F. nucleatum, as well as their genetic diversity, were evaluated in individuals with gingivitis, chronic periodontitis and periodontally healthy. In addition, the biotyping, serotyping, and prevalence of the ltx and cdt genes in A. actinomycetemcomitans were also determined. Subgingival biofilms obtained from gingivitis (70), periodontitis (75) and healthy (95) individuals were analyzed by cultures and PCR. Bacterial typing and presence of ltx and cdt genes in A. actinomycetemcomitans were also verified. DNA from A. actinomycetemcomitans and F. nucleatum was detected respectively, in 65.7% and 57.1% of gingivitis, 80% and 68% of periodontitis, and 57.8% and 37.8% of healthy. A. actinomycetemcomitans from gingivitis were biotypes I, II, IV, V, and X, and serotypes a, c, and e. In periodontitis, biotypes II, VI, and X, and serotypes a, b, and c were found. In healthy subjects, biotypes II and X, and serotypes b and c were found. The LTX and ltxA were observed in strains from gingivitis and periodontitis pockets. Subsequently, our data also showed no direct relationship between ltxA gene expression and leukotoxin gene 530-bp presence. On the other hand, cdt gene predominated during the inflammatory disease process. Our results strongly support a role of A. actinomycetemcomitans and F. nucleatum in advanced stage of periodontal disease.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Anaerobe Laboratory Department of Microbiology Institute of Biomedical Sciences University of Sao Paulo - USP, Av. Prof. Lineu PrestesPostdoctoral Research Fellow Department of Dental Materials and Prosthodontics School of Dentistry of Araraquara Sao Paulo State University - UNESP, Rua HumaitaDepartment of Biomaterials Radboud University Medical Center, Philips van Leydenlaan 25Postdoctoral Research Fellow Department of Dental Materials and Prosthodontics School of Dentistry of Araraquara Sao Paulo State University - UNESP, Rua HumaitaFAPESP: 2013/13652-6Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Radboud University Medical CenterArenas Rodrigues, Viviane Aparecidade Avila, Erica Dorigatti [UNESP]Nakano, VivianeAvila-Campos, Mario Julio2018-12-11T17:21:16Z2018-12-11T17:21:16Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article50-58application/pdfhttp://dx.doi.org/10.1016/j.anaerobe.2018.05.015Anaerobe, v. 52, p. 50-58.1095-82741075-9964http://hdl.handle.net/11449/17653810.1016/j.anaerobe.2018.05.0152-s2.0-850493444932-s2.0-85049344493.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnaerobe1,1441,144info:eu-repo/semantics/openAccess2023-12-18T06:18:51Zoai:repositorio.unesp.br:11449/176538Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-18T06:18:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions |
title |
Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions |
spellingShingle |
Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions Arenas Rodrigues, Viviane Aparecida Aggregatibacter actinomycetemcomitans Bacterial typing Fusobacterium nucleatum Oral microbiology Periodontal disease |
title_short |
Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions |
title_full |
Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions |
title_fullStr |
Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions |
title_full_unstemmed |
Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions |
title_sort |
Qualitative, quantitative and genotypic evaluation of Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum isolated from individuals with different periodontal clinical conditions |
author |
Arenas Rodrigues, Viviane Aparecida |
author_facet |
Arenas Rodrigues, Viviane Aparecida de Avila, Erica Dorigatti [UNESP] Nakano, Viviane Avila-Campos, Mario Julio |
author_role |
author |
author2 |
de Avila, Erica Dorigatti [UNESP] Nakano, Viviane Avila-Campos, Mario Julio |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Radboud University Medical Center |
dc.contributor.author.fl_str_mv |
Arenas Rodrigues, Viviane Aparecida de Avila, Erica Dorigatti [UNESP] Nakano, Viviane Avila-Campos, Mario Julio |
dc.subject.por.fl_str_mv |
Aggregatibacter actinomycetemcomitans Bacterial typing Fusobacterium nucleatum Oral microbiology Periodontal disease |
topic |
Aggregatibacter actinomycetemcomitans Bacterial typing Fusobacterium nucleatum Oral microbiology Periodontal disease |
description |
Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum are strongly associated with periodontitis, and their evaluations are relevant to understand their role in the etiology and progression of periodontal diseases. In this study, the qualitative and quantitative detection of A. actinomycetemcomitans and F. nucleatum, as well as their genetic diversity, were evaluated in individuals with gingivitis, chronic periodontitis and periodontally healthy. In addition, the biotyping, serotyping, and prevalence of the ltx and cdt genes in A. actinomycetemcomitans were also determined. Subgingival biofilms obtained from gingivitis (70), periodontitis (75) and healthy (95) individuals were analyzed by cultures and PCR. Bacterial typing and presence of ltx and cdt genes in A. actinomycetemcomitans were also verified. DNA from A. actinomycetemcomitans and F. nucleatum was detected respectively, in 65.7% and 57.1% of gingivitis, 80% and 68% of periodontitis, and 57.8% and 37.8% of healthy. A. actinomycetemcomitans from gingivitis were biotypes I, II, IV, V, and X, and serotypes a, c, and e. In periodontitis, biotypes II, VI, and X, and serotypes a, b, and c were found. In healthy subjects, biotypes II and X, and serotypes b and c were found. The LTX and ltxA were observed in strains from gingivitis and periodontitis pockets. Subsequently, our data also showed no direct relationship between ltxA gene expression and leukotoxin gene 530-bp presence. On the other hand, cdt gene predominated during the inflammatory disease process. Our results strongly support a role of A. actinomycetemcomitans and F. nucleatum in advanced stage of periodontal disease. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:21:16Z 2018-12-11T17:21:16Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anaerobe.2018.05.015 Anaerobe, v. 52, p. 50-58. 1095-8274 1075-9964 http://hdl.handle.net/11449/176538 10.1016/j.anaerobe.2018.05.015 2-s2.0-85049344493 2-s2.0-85049344493.pdf |
url |
http://dx.doi.org/10.1016/j.anaerobe.2018.05.015 http://hdl.handle.net/11449/176538 |
identifier_str_mv |
Anaerobe, v. 52, p. 50-58. 1095-8274 1075-9964 10.1016/j.anaerobe.2018.05.015 2-s2.0-85049344493 2-s2.0-85049344493.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anaerobe 1,144 1,144 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
50-58 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965328931815424 |