Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/224903 |
Resumo: | To determine the inhibitory Qacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of Lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. Of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. Isolates identified as L. reuteri, L. salivarius, or Lactobacillus spp. inhibited Enterococcus faecalis, E. faecium, Listeria monocytogenes, and Salmonella spp. but not L. casei, L. delbrueckii, L. fermentum, or L. helveticus by the well-diffusion simultaneous antagonism method under anaerobic incubation conditions. The antagonistic substances produced by some of the Lactobacillus isolates were inactivated after treatment by proteolytic enzymes, which suggested that the substances could be antimicrobial peptides or bacteriocins. |
id |
UNSP_95785a2c58cff2d2b67d716013a70d65 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/224903 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickensTo determine the inhibitory Qacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of Lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. Of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. Isolates identified as L. reuteri, L. salivarius, or Lactobacillus spp. inhibited Enterococcus faecalis, E. faecium, Listeria monocytogenes, and Salmonella spp. but not L. casei, L. delbrueckii, L. fermentum, or L. helveticus by the well-diffusion simultaneous antagonism method under anaerobic incubation conditions. The antagonistic substances produced by some of the Lactobacillus isolates were inactivated after treatment by proteolytic enzymes, which suggested that the substances could be antimicrobial peptides or bacteriocins.Department of Clinical Veterinary Medicine College of Veterinary Medicine and Animal Science São Paulo State University, Distrito Rubião Junior S/N, Botucatu, São PauloDepartment of Biostatistics Institute for the Biosciences São Paulo State University, Distrito Rubião Junior S/N, Botucatu, São PauloDepartment of Clinical Veterinary Medicine College of Veterinary Medicine and Animal Science São Paulo State University, Distrito Rubião Junior S/N, Botucatu, São PauloDepartment of Biostatistics Institute for the Biosciences São Paulo State University, Distrito Rubião Junior S/N, Botucatu, São PauloUniversidade Estadual Paulista (UNESP)Lima, Edna T. [UNESP]Andreatti Filho, Raphael L. [UNESP]Okamoto, Adriano S. [UNESP]Noujaim, José C. [UNESP]Barros, Mércia R. [UNESP]Crocci, Adalberto J. [UNESP]2022-04-28T20:17:19Z2022-04-28T20:17:19Z2007-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article103-107Canadian Journal of Veterinary Research, v. 71, n. 2, p. 103-107, 2007.0830-9000http://hdl.handle.net/11449/2249032-s2.0-34248328002Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCanadian Journal of Veterinary Researchinfo:eu-repo/semantics/openAccess2022-04-28T20:17:19Zoai:repositorio.unesp.br:11449/224903Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:26:17.729427Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens |
title |
Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens |
spellingShingle |
Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens Lima, Edna T. [UNESP] |
title_short |
Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens |
title_full |
Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens |
title_fullStr |
Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens |
title_full_unstemmed |
Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens |
title_sort |
Evaluation in vitro of the antagonistic substances produced by Lactobacillus spp. isolated from chickens |
author |
Lima, Edna T. [UNESP] |
author_facet |
Lima, Edna T. [UNESP] Andreatti Filho, Raphael L. [UNESP] Okamoto, Adriano S. [UNESP] Noujaim, José C. [UNESP] Barros, Mércia R. [UNESP] Crocci, Adalberto J. [UNESP] |
author_role |
author |
author2 |
Andreatti Filho, Raphael L. [UNESP] Okamoto, Adriano S. [UNESP] Noujaim, José C. [UNESP] Barros, Mércia R. [UNESP] Crocci, Adalberto J. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Lima, Edna T. [UNESP] Andreatti Filho, Raphael L. [UNESP] Okamoto, Adriano S. [UNESP] Noujaim, José C. [UNESP] Barros, Mércia R. [UNESP] Crocci, Adalberto J. [UNESP] |
description |
To determine the inhibitory Qacity of lactic acid bacteria due to the action of antagonistic substances, we tested 474 isolates of Lactobacillus from the crop and cecum of chickens against gram-positive and gram-negative indicator microorganisms by the spot-on-the-lawn and well-diffusion antagonism methods. Of the 474 isolates, 265 demonstrated antimicrobial activity against the indicator microorganisms. Isolates identified as L. reuteri, L. salivarius, or Lactobacillus spp. inhibited Enterococcus faecalis, E. faecium, Listeria monocytogenes, and Salmonella spp. but not L. casei, L. delbrueckii, L. fermentum, or L. helveticus by the well-diffusion simultaneous antagonism method under anaerobic incubation conditions. The antagonistic substances produced by some of the Lactobacillus isolates were inactivated after treatment by proteolytic enzymes, which suggested that the substances could be antimicrobial peptides or bacteriocins. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-04-01 2022-04-28T20:17:19Z 2022-04-28T20:17:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Canadian Journal of Veterinary Research, v. 71, n. 2, p. 103-107, 2007. 0830-9000 http://hdl.handle.net/11449/224903 2-s2.0-34248328002 |
identifier_str_mv |
Canadian Journal of Veterinary Research, v. 71, n. 2, p. 103-107, 2007. 0830-9000 2-s2.0-34248328002 |
url |
http://hdl.handle.net/11449/224903 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Canadian Journal of Veterinary Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
103-107 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129069734166528 |