Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1136/vr.103607 http://hdl.handle.net/11449/161254 |
Resumo: | This study compares two methods for seminal plasma removal by evaluating sperm recovery rates, and motility and morphology of cooled-stored semen. Ejaculates were divided into three groups: control, filtration and cushioned centrifugation. Semen was extended to 25 million sperm/ml using a skim-milk-based extender and stored at 5 degrees C for all groups. Sperm motility (total motility (% TM) and progressive motility (% PM)) was determined at 0, 24, 48 and 72 hours by a computer-assisted sperm analyser. Sperm morphology was assessed using differential interference microscopy. Overall, % TM of the centrifugation group was significantly higher than the filter group, but not significantly different than the control. No significant difference in % TM or % PM was detected for the control group and filter. Cushioned centrifugation was a superior method to obtain progressively motile sperm compared with control (P = 0.03) and filter groups (P < 0.001). No significant difference was found for the per cent of normal sperm cells and detached heads between the groups. This study demonstrated that cushioned centrifugation was a superior method to remove seminal plasma while preserving % TM and enhancing % PM for stallions under cooled storage over three days. However, as the differences appear to be negligible, the SpermFilter may represent an alternative for farms lacking a centrifuge. |
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spelling |
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semenThis study compares two methods for seminal plasma removal by evaluating sperm recovery rates, and motility and morphology of cooled-stored semen. Ejaculates were divided into three groups: control, filtration and cushioned centrifugation. Semen was extended to 25 million sperm/ml using a skim-milk-based extender and stored at 5 degrees C for all groups. Sperm motility (total motility (% TM) and progressive motility (% PM)) was determined at 0, 24, 48 and 72 hours by a computer-assisted sperm analyser. Sperm morphology was assessed using differential interference microscopy. Overall, % TM of the centrifugation group was significantly higher than the filter group, but not significantly different than the control. No significant difference in % TM or % PM was detected for the control group and filter. Cushioned centrifugation was a superior method to obtain progressively motile sperm compared with control (P = 0.03) and filter groups (P < 0.001). No significant difference was found for the per cent of normal sperm cells and detached heads between the groups. This study demonstrated that cushioned centrifugation was a superior method to remove seminal plasma while preserving % TM and enhancing % PM for stallions under cooled storage over three days. However, as the differences appear to be negligible, the SpermFilter may represent an alternative for farms lacking a centrifuge.Rood & Riddle Equine Hosp, 2150 Georgetown Rd, Lexington, KY 40511 USAUniv Illinois, Coll Vet Med, Dept Vet Clin Med, 1008 West Hazelwood Dr, Urbana, IL 61802 USAUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Reprod Anim & Radiol, Sao Paulo, BrazilUniv Illinois, Dept Anim Sci, 1207 W Gregory Dr, Urbana, IL 61801 USAUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Reprod Anim & Radiol, Sao Paulo, BrazilBmj Publishing GroupRood & Riddle Equine HospUniv IllinoisUniversidade Estadual Paulista (Unesp)Roach, J.Schnobrich, M.Ellerbrock, R.Feijo, L.Bradecamp, E.Alvarenga, M. A. [UNESP]Kline, K.Canisso, I.2018-11-26T16:27:46Z2018-11-26T16:27:46Z2016-03-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttp://dx.doi.org/10.1136/vr.103607Veterinary Record. London: Bmj Publishing Group, v. 178, n. 10, 4 p., 2016.0042-4900http://hdl.handle.net/11449/16125410.1136/vr.103607WOS:000371220500019WOS000371220500019.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Record0,464info:eu-repo/semantics/openAccess2024-09-09T14:06:04Zoai:repositorio.unesp.br:11449/161254Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen |
title |
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen |
spellingShingle |
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen Roach, J. |
title_short |
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen |
title_full |
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen |
title_fullStr |
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen |
title_full_unstemmed |
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen |
title_sort |
Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen |
author |
Roach, J. |
author_facet |
Roach, J. Schnobrich, M. Ellerbrock, R. Feijo, L. Bradecamp, E. Alvarenga, M. A. [UNESP] Kline, K. Canisso, I. |
author_role |
author |
author2 |
Schnobrich, M. Ellerbrock, R. Feijo, L. Bradecamp, E. Alvarenga, M. A. [UNESP] Kline, K. Canisso, I. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Rood & Riddle Equine Hosp Univ Illinois Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Roach, J. Schnobrich, M. Ellerbrock, R. Feijo, L. Bradecamp, E. Alvarenga, M. A. [UNESP] Kline, K. Canisso, I. |
description |
This study compares two methods for seminal plasma removal by evaluating sperm recovery rates, and motility and morphology of cooled-stored semen. Ejaculates were divided into three groups: control, filtration and cushioned centrifugation. Semen was extended to 25 million sperm/ml using a skim-milk-based extender and stored at 5 degrees C for all groups. Sperm motility (total motility (% TM) and progressive motility (% PM)) was determined at 0, 24, 48 and 72 hours by a computer-assisted sperm analyser. Sperm morphology was assessed using differential interference microscopy. Overall, % TM of the centrifugation group was significantly higher than the filter group, but not significantly different than the control. No significant difference in % TM or % PM was detected for the control group and filter. Cushioned centrifugation was a superior method to obtain progressively motile sperm compared with control (P = 0.03) and filter groups (P < 0.001). No significant difference was found for the per cent of normal sperm cells and detached heads between the groups. This study demonstrated that cushioned centrifugation was a superior method to remove seminal plasma while preserving % TM and enhancing % PM for stallions under cooled storage over three days. However, as the differences appear to be negligible, the SpermFilter may represent an alternative for farms lacking a centrifuge. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-05 2018-11-26T16:27:46Z 2018-11-26T16:27:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1136/vr.103607 Veterinary Record. London: Bmj Publishing Group, v. 178, n. 10, 4 p., 2016. 0042-4900 http://hdl.handle.net/11449/161254 10.1136/vr.103607 WOS:000371220500019 WOS000371220500019.pdf |
url |
http://dx.doi.org/10.1136/vr.103607 http://hdl.handle.net/11449/161254 |
identifier_str_mv |
Veterinary Record. London: Bmj Publishing Group, v. 178, n. 10, 4 p., 2016. 0042-4900 10.1136/vr.103607 WOS:000371220500019 WOS000371220500019.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Record 0,464 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4 application/pdf |
dc.publisher.none.fl_str_mv |
Bmj Publishing Group |
publisher.none.fl_str_mv |
Bmj Publishing Group |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021346709602304 |