Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture

Detalhes bibliográficos
Autor(a) principal: Simão, Vinícius Augusto
Data de Publicação: 2019
Outros Autores: Evangelista-Ribeiro, Caetano Pedro [UNESP], Brand, Heloisa [UNESP], Lagass-Pereira, Lougan, Marques, Laís Fernanda, Benites-Aoki, Pedro Henrique [UNESP], Nunes da Silveira-Antunes, Roseli, Tonso, Aldo, Ribeiro-Paes, João Tadeu [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.biologicals.2019.08.006
http://hdl.handle.net/11449/188050
Resumo: Adipose-derived mesenchymal stromal/stem cells (ASC) have acquired a prominent role in tissue engineering and regenerative medicine. However, the standardization of basic culture procedures in this cellular type is still not well established according to the main qualitative cellular attributes. We evaluate the cell growth profile of human ASC in a different culture medium volumes and their nutritional composition utilizing static cultivation. Culture medium volumes (5, 10 and 15 mL/25 cm2) in T-flasks were evaluated by kinetic parameters and the metabolic composition was determined by biochemical analysis and Fourier transform infrared (FT-IR) absorption spectroscopy. 50% renewal of culture medium volume every 48 h was adopted. Immunophenotypic characterization and cell differentiation were performed. There was no difference (p > 0.05) in the kinetic parameters of cell proliferation between the culture medium volumes or in FT-IR composition. However, the concentrations of glucose, glutamine, lactate, and glutamate varied significantly during the cultivation process as a function of the medium volume. ASC presented specific antigens and differentiation potential of mesenchymal stromal/stem cells. It was concluded that the minimal culture medium volume (5 mL/25 cm2 in static culture) was sufficient to maintain the stability, potency, and growth of ASC, representing an economic and safe standardization for this cell culture process.
id UNSP_99405ebd90c5a00a342d36f94401612e
oai_identifier_str oai:repositorio.unesp.br:11449/188050
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static cultureBiochemical analysisFT-IRKinetic evaluationMedium compositionStandard cultivationStem cellsAdipose-derived mesenchymal stromal/stem cells (ASC) have acquired a prominent role in tissue engineering and regenerative medicine. However, the standardization of basic culture procedures in this cellular type is still not well established according to the main qualitative cellular attributes. We evaluate the cell growth profile of human ASC in a different culture medium volumes and their nutritional composition utilizing static cultivation. Culture medium volumes (5, 10 and 15 mL/25 cm2) in T-flasks were evaluated by kinetic parameters and the metabolic composition was determined by biochemical analysis and Fourier transform infrared (FT-IR) absorption spectroscopy. 50% renewal of culture medium volume every 48 h was adopted. Immunophenotypic characterization and cell differentiation were performed. There was no difference (p > 0.05) in the kinetic parameters of cell proliferation between the culture medium volumes or in FT-IR composition. However, the concentrations of glucose, glutamine, lactate, and glutamate varied significantly during the cultivation process as a function of the medium volume. ASC presented specific antigens and differentiation potential of mesenchymal stromal/stem cells. It was concluded that the minimal culture medium volume (5 mL/25 cm2 in static culture) was sufficient to maintain the stability, potency, and growth of ASC, representing an economic and safe standardization for this cell culture process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Medicine University of São PauloSchool of Sciences Humanities and Languages São Paulo State University (UNESP)Biomedical Science Institute University of São PauloSchool of Medicine of Marilia HemocenterPolytechnic School University of São PauloSchool of Sciences Humanities and Languages São Paulo State University (UNESP)FAPESP: #2014/03324–4Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)HemocenterSimão, Vinícius AugustoEvangelista-Ribeiro, Caetano Pedro [UNESP]Brand, Heloisa [UNESP]Lagass-Pereira, LouganMarques, Laís FernandaBenites-Aoki, Pedro Henrique [UNESP]Nunes da Silveira-Antunes, RoseliTonso, AldoRibeiro-Paes, João Tadeu [UNESP]2019-10-06T15:55:44Z2019-10-06T15:55:44Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.biologicals.2019.08.006Biologicals.1095-83201045-1056http://hdl.handle.net/11449/18805010.1016/j.biologicals.2019.08.0062-s2.0-85071850791Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiologicalsinfo:eu-repo/semantics/openAccess2021-10-22T18:56:48Zoai:repositorio.unesp.br:11449/188050Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:56:03.995907Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture
title Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture
spellingShingle Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture
Simão, Vinícius Augusto
Biochemical analysis
FT-IR
Kinetic evaluation
Medium composition
Standard cultivation
Stem cells
title_short Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture
title_full Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture
title_fullStr Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture
title_full_unstemmed Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture
title_sort Metabolic and proliferation evaluation of human adipose-derived mesenchymal stromal cells (ASC) in different culture medium volumes: standardization of static culture
author Simão, Vinícius Augusto
author_facet Simão, Vinícius Augusto
Evangelista-Ribeiro, Caetano Pedro [UNESP]
Brand, Heloisa [UNESP]
Lagass-Pereira, Lougan
Marques, Laís Fernanda
Benites-Aoki, Pedro Henrique [UNESP]
Nunes da Silveira-Antunes, Roseli
Tonso, Aldo
Ribeiro-Paes, João Tadeu [UNESP]
author_role author
author2 Evangelista-Ribeiro, Caetano Pedro [UNESP]
Brand, Heloisa [UNESP]
Lagass-Pereira, Lougan
Marques, Laís Fernanda
Benites-Aoki, Pedro Henrique [UNESP]
Nunes da Silveira-Antunes, Roseli
Tonso, Aldo
Ribeiro-Paes, João Tadeu [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Hemocenter
dc.contributor.author.fl_str_mv Simão, Vinícius Augusto
Evangelista-Ribeiro, Caetano Pedro [UNESP]
Brand, Heloisa [UNESP]
Lagass-Pereira, Lougan
Marques, Laís Fernanda
Benites-Aoki, Pedro Henrique [UNESP]
Nunes da Silveira-Antunes, Roseli
Tonso, Aldo
Ribeiro-Paes, João Tadeu [UNESP]
dc.subject.por.fl_str_mv Biochemical analysis
FT-IR
Kinetic evaluation
Medium composition
Standard cultivation
Stem cells
topic Biochemical analysis
FT-IR
Kinetic evaluation
Medium composition
Standard cultivation
Stem cells
description Adipose-derived mesenchymal stromal/stem cells (ASC) have acquired a prominent role in tissue engineering and regenerative medicine. However, the standardization of basic culture procedures in this cellular type is still not well established according to the main qualitative cellular attributes. We evaluate the cell growth profile of human ASC in a different culture medium volumes and their nutritional composition utilizing static cultivation. Culture medium volumes (5, 10 and 15 mL/25 cm2) in T-flasks were evaluated by kinetic parameters and the metabolic composition was determined by biochemical analysis and Fourier transform infrared (FT-IR) absorption spectroscopy. 50% renewal of culture medium volume every 48 h was adopted. Immunophenotypic characterization and cell differentiation were performed. There was no difference (p > 0.05) in the kinetic parameters of cell proliferation between the culture medium volumes or in FT-IR composition. However, the concentrations of glucose, glutamine, lactate, and glutamate varied significantly during the cultivation process as a function of the medium volume. ASC presented specific antigens and differentiation potential of mesenchymal stromal/stem cells. It was concluded that the minimal culture medium volume (5 mL/25 cm2 in static culture) was sufficient to maintain the stability, potency, and growth of ASC, representing an economic and safe standardization for this cell culture process.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T15:55:44Z
2019-10-06T15:55:44Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.biologicals.2019.08.006
Biologicals.
1095-8320
1045-1056
http://hdl.handle.net/11449/188050
10.1016/j.biologicals.2019.08.006
2-s2.0-85071850791
url http://dx.doi.org/10.1016/j.biologicals.2019.08.006
http://hdl.handle.net/11449/188050
identifier_str_mv Biologicals.
1095-8320
1045-1056
10.1016/j.biologicals.2019.08.006
2-s2.0-85071850791
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biologicals
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128292342988800