AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)

Detalhes bibliográficos
Autor(a) principal: Giannoni, M. L.
Data de Publicação: 1993
Outros Autores: Foresti, F., Falcone, C., Tosta, P. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.gmb.org.br/Revistas/V16/v16a044.pdf
http://hdl.handle.net/11449/35455
Resumo: In order to develop an efficient and low-cost technique for obtaining bird chromosome preparations, and to adapt the cytogenetic process of bird sexing for general use at zoos and breeding farms with the technical support of cytogenetics laboratories, we tested variants of the technique described by Giannoni et al. (Genet. Sel. Evol. 23: 123-125, 1991), based on the utilization of cellular material from growing feather pulp cultured in complete medium for six hours. Hanks' saline solution gave satisfactory performance as a substitute for complete medium, with no need to use PHA, serum of collagenase, when utilized in material obtained from feather pulp of Amazona amazonica (Psittacidae).
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spelling AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)In order to develop an efficient and low-cost technique for obtaining bird chromosome preparations, and to adapt the cytogenetic process of bird sexing for general use at zoos and breeding farms with the technical support of cytogenetics laboratories, we tested variants of the technique described by Giannoni et al. (Genet. Sel. Evol. 23: 123-125, 1991), based on the utilization of cellular material from growing feather pulp cultured in complete medium for six hours. Hanks' saline solution gave satisfactory performance as a substitute for complete medium, with no need to use PHA, serum of collagenase, when utilized in material obtained from feather pulp of Amazona amazonica (Psittacidae).UNESP,FCAVJ,DEPT MELHORAMENTO GENET ANIMAL,BR-14870 JABOTICABAL,SP,BRAZILUNESP,FCAVJ,DEPT MELHORAMENTO GENET ANIMAL,BR-14870 JABOTICABAL,SP,BRAZILSoc Brasil GeneticaUniversidade Estadual Paulista (Unesp)Giannoni, M. L.Foresti, F.Falcone, C.Tosta, P. A.2014-05-20T15:24:58Z2014-05-20T15:24:58Z1993-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article477-483application/pdfhttp://www.gmb.org.br/Revistas/V16/v16a044.pdfRevista Brasileira de Genetica. Ribeirao Pret: Soc Brasil Genetica, v. 16, n. 2, p. 477-483, 1993.0100-8455http://hdl.handle.net/11449/35455WOS:A1993LJ96500020WOSA1993LJ96500020.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Genéticainfo:eu-repo/semantics/openAccess2024-06-07T18:40:52Zoai:repositorio.unesp.br:11449/35455Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-07T18:40:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)
title AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)
spellingShingle AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)
Giannoni, M. L.
title_short AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)
title_full AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)
title_fullStr AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)
title_full_unstemmed AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)
title_sort AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)
author Giannoni, M. L.
author_facet Giannoni, M. L.
Foresti, F.
Falcone, C.
Tosta, P. A.
author_role author
author2 Foresti, F.
Falcone, C.
Tosta, P. A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Giannoni, M. L.
Foresti, F.
Falcone, C.
Tosta, P. A.
description In order to develop an efficient and low-cost technique for obtaining bird chromosome preparations, and to adapt the cytogenetic process of bird sexing for general use at zoos and breeding farms with the technical support of cytogenetics laboratories, we tested variants of the technique described by Giannoni et al. (Genet. Sel. Evol. 23: 123-125, 1991), based on the utilization of cellular material from growing feather pulp cultured in complete medium for six hours. Hanks' saline solution gave satisfactory performance as a substitute for complete medium, with no need to use PHA, serum of collagenase, when utilized in material obtained from feather pulp of Amazona amazonica (Psittacidae).
publishDate 1993
dc.date.none.fl_str_mv 1993-06-01
2014-05-20T15:24:58Z
2014-05-20T15:24:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.gmb.org.br/Revistas/V16/v16a044.pdf
Revista Brasileira de Genetica. Ribeirao Pret: Soc Brasil Genetica, v. 16, n. 2, p. 477-483, 1993.
0100-8455
http://hdl.handle.net/11449/35455
WOS:A1993LJ96500020
WOSA1993LJ96500020.pdf
url http://www.gmb.org.br/Revistas/V16/v16a044.pdf
http://hdl.handle.net/11449/35455
identifier_str_mv Revista Brasileira de Genetica. Ribeirao Pret: Soc Brasil Genetica, v. 16, n. 2, p. 477-483, 1993.
0100-8455
WOS:A1993LJ96500020
WOSA1993LJ96500020.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Genética
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 477-483
application/pdf
dc.publisher.none.fl_str_mv Soc Brasil Genetica
publisher.none.fl_str_mv Soc Brasil Genetica
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803649579276566528

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