AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)
Autor(a) principal: | |
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Data de Publicação: | 1993 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.gmb.org.br/Revistas/V16/v16a044.pdf http://hdl.handle.net/11449/35455 |
Resumo: | In order to develop an efficient and low-cost technique for obtaining bird chromosome preparations, and to adapt the cytogenetic process of bird sexing for general use at zoos and breeding farms with the technical support of cytogenetics laboratories, we tested variants of the technique described by Giannoni et al. (Genet. Sel. Evol. 23: 123-125, 1991), based on the utilization of cellular material from growing feather pulp cultured in complete medium for six hours. Hanks' saline solution gave satisfactory performance as a substitute for complete medium, with no need to use PHA, serum of collagenase, when utilized in material obtained from feather pulp of Amazona amazonica (Psittacidae). |
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Repositório Institucional da UNESP |
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2946 |
spelling |
AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE)In order to develop an efficient and low-cost technique for obtaining bird chromosome preparations, and to adapt the cytogenetic process of bird sexing for general use at zoos and breeding farms with the technical support of cytogenetics laboratories, we tested variants of the technique described by Giannoni et al. (Genet. Sel. Evol. 23: 123-125, 1991), based on the utilization of cellular material from growing feather pulp cultured in complete medium for six hours. Hanks' saline solution gave satisfactory performance as a substitute for complete medium, with no need to use PHA, serum of collagenase, when utilized in material obtained from feather pulp of Amazona amazonica (Psittacidae).UNESP,FCAVJ,DEPT MELHORAMENTO GENET ANIMAL,BR-14870 JABOTICABAL,SP,BRAZILUNESP,FCAVJ,DEPT MELHORAMENTO GENET ANIMAL,BR-14870 JABOTICABAL,SP,BRAZILSoc Brasil GeneticaUniversidade Estadual Paulista (Unesp)Giannoni, M. L.Foresti, F.Falcone, C.Tosta, P. A.2014-05-20T15:24:58Z2014-05-20T15:24:58Z1993-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article477-483application/pdfhttp://www.gmb.org.br/Revistas/V16/v16a044.pdfRevista Brasileira de Genetica. Ribeirao Pret: Soc Brasil Genetica, v. 16, n. 2, p. 477-483, 1993.0100-8455http://hdl.handle.net/11449/35455WOS:A1993LJ96500020WOSA1993LJ96500020.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Genéticainfo:eu-repo/semantics/openAccess2024-06-07T18:40:52Zoai:repositorio.unesp.br:11449/35455Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:31:58.303363Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE) |
title |
AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE) |
spellingShingle |
AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE) Giannoni, M. L. |
title_short |
AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE) |
title_full |
AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE) |
title_fullStr |
AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE) |
title_full_unstemmed |
AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE) |
title_sort |
AN INEXPENSIVE METHOD FOR CHROMOSOME PREPARATIONS FROM FEATHER PULP IN BIRDS, USING SHORT-TERM TREATMENT WITH COLCHICINE IN-VITRO, DEMONSTRATED ON AMAZONA-AMAZONICA (PSITTACIDAE) |
author |
Giannoni, M. L. |
author_facet |
Giannoni, M. L. Foresti, F. Falcone, C. Tosta, P. A. |
author_role |
author |
author2 |
Foresti, F. Falcone, C. Tosta, P. A. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Giannoni, M. L. Foresti, F. Falcone, C. Tosta, P. A. |
description |
In order to develop an efficient and low-cost technique for obtaining bird chromosome preparations, and to adapt the cytogenetic process of bird sexing for general use at zoos and breeding farms with the technical support of cytogenetics laboratories, we tested variants of the technique described by Giannoni et al. (Genet. Sel. Evol. 23: 123-125, 1991), based on the utilization of cellular material from growing feather pulp cultured in complete medium for six hours. Hanks' saline solution gave satisfactory performance as a substitute for complete medium, with no need to use PHA, serum of collagenase, when utilized in material obtained from feather pulp of Amazona amazonica (Psittacidae). |
publishDate |
1993 |
dc.date.none.fl_str_mv |
1993-06-01 2014-05-20T15:24:58Z 2014-05-20T15:24:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.gmb.org.br/Revistas/V16/v16a044.pdf Revista Brasileira de Genetica. Ribeirao Pret: Soc Brasil Genetica, v. 16, n. 2, p. 477-483, 1993. 0100-8455 http://hdl.handle.net/11449/35455 WOS:A1993LJ96500020 WOSA1993LJ96500020.pdf |
url |
http://www.gmb.org.br/Revistas/V16/v16a044.pdf http://hdl.handle.net/11449/35455 |
identifier_str_mv |
Revista Brasileira de Genetica. Ribeirao Pret: Soc Brasil Genetica, v. 16, n. 2, p. 477-483, 1993. 0100-8455 WOS:A1993LJ96500020 WOSA1993LJ96500020.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Genética |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
477-483 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasil Genetica |
publisher.none.fl_str_mv |
Soc Brasil Genetica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128667558084608 |