Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12861-017-0151-3 http://hdl.handle.net/11449/162992 |
Resumo: | Background: Mitochondrial alternative respiratory-chain enzymes are phylogenetically widespread, and buffer stresses affecting oxidative phosphorylation in species that possess them. However, they have been lost in the evolutionary lineages leading to vertebrates and arthropods, raising the question as to what survival or reproductive disadvantages they confer. Recent interest in using them in therapy lends a biomedical dimension to this question. Methods: Here, we examined the impact of the expression of Ciona intestinalis alternative oxidase, AOX, on the reproductive success of Drosophila melanogaster males. Sperm-competition assays were performed between flies carrying three copies of a ubiquitously expressed AOX construct, driven by the a-tubulin promoter, and wild-type males of the same genetic background. Results: In sperm-competition assays, AOX conferred a substantial disadvantage, associated with decreased production of mature sperm. Sperm differentiation appeared to proceed until the last stages, but was spatially deranged, with spermatozoids retained in the testis instead of being released to the seminal vesicle. High AOX expression was detected in the outermost cell-layer of the testis sheath, which we hypothesize may disrupt a signal required for sperm maturation. Conclusions: AOX expression in Drosophila thus has effects that are deleterious to male reproductive function. Our results imply that AOX therapy must be developed with caution. |
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Repositório Institucional da UNESP |
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Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogasterMitochondriaRespiratory chainSpermatogenesisSperm competitionBackground: Mitochondrial alternative respiratory-chain enzymes are phylogenetically widespread, and buffer stresses affecting oxidative phosphorylation in species that possess them. However, they have been lost in the evolutionary lineages leading to vertebrates and arthropods, raising the question as to what survival or reproductive disadvantages they confer. Recent interest in using them in therapy lends a biomedical dimension to this question. Methods: Here, we examined the impact of the expression of Ciona intestinalis alternative oxidase, AOX, on the reproductive success of Drosophila melanogaster males. Sperm-competition assays were performed between flies carrying three copies of a ubiquitously expressed AOX construct, driven by the a-tubulin promoter, and wild-type males of the same genetic background. Results: In sperm-competition assays, AOX conferred a substantial disadvantage, associated with decreased production of mature sperm. Sperm differentiation appeared to proceed until the last stages, but was spatially deranged, with spermatozoids retained in the testis instead of being released to the seminal vesicle. High AOX expression was detected in the outermost cell-layer of the testis sheath, which we hypothesize may disrupt a signal required for sperm maturation. Conclusions: AOX expression in Drosophila thus has effects that are deleterious to male reproductive function. Our results imply that AOX therapy must be developed with caution.European Union (Marie Curie International Incoming Fellowship)European Research Council Advanced GrantFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Finnish Cultural FoundationAcademy of FinlandTampere University Medical Research FundSigrid Juselius FoundationUniv Tampere, Tampere Univ Hosp, Inst Biosci & Med Technol, FI-33014 Tampere, FinlandUniv Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, BrazilUniv Helsinki, Inst Biotechnol, FI-00014 Helsinki, FinlandUniv Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, BrazilEuropean Union (Marie Curie International Incoming Fellowship): GA328988European Research Council Advanced Grant: 232,738FAPESP: 2014/02253-6Academy of Finland: 256,615Academy of Finland: 272,376Biomed Central LtdUniv TampereUniversidade Estadual Paulista (Unesp)Univ HelsinkiSaari, SinaAndjelkovic, AnaGarcia, Geovana S. [UNESP]Jacobs, Howard T.Oliveira, Marcos T. [UNESP]2018-11-26T17:39:40Z2018-11-26T17:39:40Z2017-07-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1186/s12861-017-0151-3Bmc Developmental Biology. London: Biomed Central Ltd, v. 17, 11 p., 2017.1471-213Xhttp://hdl.handle.net/11449/16299210.1186/s12861-017-0151-3WOS:000405439300001WOS000405439300001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Developmental Biologyinfo:eu-repo/semantics/openAccess2024-06-07T15:31:35Zoai:repositorio.unesp.br:11449/162992Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:19:49.294097Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster |
title |
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster |
spellingShingle |
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster Saari, Sina Mitochondria Respiratory chain Spermatogenesis Sperm competition |
title_short |
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster |
title_full |
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster |
title_fullStr |
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster |
title_full_unstemmed |
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster |
title_sort |
Expression of Ciona intestinalis AOX causes male reproductive defects in Drosophila melanogaster |
author |
Saari, Sina |
author_facet |
Saari, Sina Andjelkovic, Ana Garcia, Geovana S. [UNESP] Jacobs, Howard T. Oliveira, Marcos T. [UNESP] |
author_role |
author |
author2 |
Andjelkovic, Ana Garcia, Geovana S. [UNESP] Jacobs, Howard T. Oliveira, Marcos T. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Univ Tampere Universidade Estadual Paulista (Unesp) Univ Helsinki |
dc.contributor.author.fl_str_mv |
Saari, Sina Andjelkovic, Ana Garcia, Geovana S. [UNESP] Jacobs, Howard T. Oliveira, Marcos T. [UNESP] |
dc.subject.por.fl_str_mv |
Mitochondria Respiratory chain Spermatogenesis Sperm competition |
topic |
Mitochondria Respiratory chain Spermatogenesis Sperm competition |
description |
Background: Mitochondrial alternative respiratory-chain enzymes are phylogenetically widespread, and buffer stresses affecting oxidative phosphorylation in species that possess them. However, they have been lost in the evolutionary lineages leading to vertebrates and arthropods, raising the question as to what survival or reproductive disadvantages they confer. Recent interest in using them in therapy lends a biomedical dimension to this question. Methods: Here, we examined the impact of the expression of Ciona intestinalis alternative oxidase, AOX, on the reproductive success of Drosophila melanogaster males. Sperm-competition assays were performed between flies carrying three copies of a ubiquitously expressed AOX construct, driven by the a-tubulin promoter, and wild-type males of the same genetic background. Results: In sperm-competition assays, AOX conferred a substantial disadvantage, associated with decreased production of mature sperm. Sperm differentiation appeared to proceed until the last stages, but was spatially deranged, with spermatozoids retained in the testis instead of being released to the seminal vesicle. High AOX expression was detected in the outermost cell-layer of the testis sheath, which we hypothesize may disrupt a signal required for sperm maturation. Conclusions: AOX expression in Drosophila thus has effects that are deleterious to male reproductive function. Our results imply that AOX therapy must be developed with caution. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-03 2018-11-26T17:39:40Z 2018-11-26T17:39:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12861-017-0151-3 Bmc Developmental Biology. London: Biomed Central Ltd, v. 17, 11 p., 2017. 1471-213X http://hdl.handle.net/11449/162992 10.1186/s12861-017-0151-3 WOS:000405439300001 WOS000405439300001.pdf |
url |
http://dx.doi.org/10.1186/s12861-017-0151-3 http://hdl.handle.net/11449/162992 |
identifier_str_mv |
Bmc Developmental Biology. London: Biomed Central Ltd, v. 17, 11 p., 2017. 1471-213X 10.1186/s12861-017-0151-3 WOS:000405439300001 WOS000405439300001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Developmental Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128634319273984 |