Paternity identification in sugarcane polycrosses by using microsatellite markers

Detalhes bibliográficos
Autor(a) principal: Xavier, M. A.
Data de Publicação: 2014
Outros Autores: Pinto, L. R., Favero, T. M., Perecin, D. [UNESP], Carlini-Garcia, L. A., Landell, M. G. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4238/2014.March.31.7
http://hdl.handle.net/11449/112279
Resumo: Although polycrosses have been used to test the potential of cross-combination of a large number of sugarcane parents, the male parent of the half-sib progenies produced is unknown. The present study aimed to integrate the molecular marker technology to the sugarcane polycross approach by the application of microsatellite markers to identify the male parent of 41 elite clones derived from polycross families. Ten microsatellite [single sequence repeats (SSRs)] primer pairs were used to identify the most likely male parent considering markers present in the selected clone but absent in the female parent. The number of alleles generated by the 10 microsatellite primer pairs ranged from 102 (cross-pollination lantern 4) to 120 (cross-pollination lantern 2) with an average of 113.25 alleles per SSR. The average genetic similarity among the involved parents in the polycrosses was 45.9%. The results of the analysis of the SSR markers absent in the female parent and present only in the selected clone as well as the genetic similarity values allowed the identification of the most likely male parent in 73% of the total clones evaluated and also to detect probable contaminations. The obtained results highlight the importance of using molecular marker technology in the identification and confirmation of the male parent of high-performance clones derived from polycrosses in the sugarcane breeding programs.
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spelling Paternity identification in sugarcane polycrosses by using microsatellite markersProgeniesSugarcane breedingGenetic dissimilarityAlthough polycrosses have been used to test the potential of cross-combination of a large number of sugarcane parents, the male parent of the half-sib progenies produced is unknown. The present study aimed to integrate the molecular marker technology to the sugarcane polycross approach by the application of microsatellite markers to identify the male parent of 41 elite clones derived from polycross families. Ten microsatellite [single sequence repeats (SSRs)] primer pairs were used to identify the most likely male parent considering markers present in the selected clone but absent in the female parent. The number of alleles generated by the 10 microsatellite primer pairs ranged from 102 (cross-pollination lantern 4) to 120 (cross-pollination lantern 2) with an average of 113.25 alleles per SSR. The average genetic similarity among the involved parents in the polycrosses was 45.9%. The results of the analysis of the SSR markers absent in the female parent and present only in the selected clone as well as the genetic similarity values allowed the identification of the most likely male parent in 73% of the total clones evaluated and also to detect probable contaminations. The obtained results highlight the importance of using molecular marker technology in the identification and confirmation of the male parent of high-performance clones derived from polycrosses in the sugarcane breeding programs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)IAC (Instituto Agronomico de Campinas)Ctr Avancado Pesquisa Tecnol Agronegocio Cana IAC, Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Fac Ciencias Agr Vet, Dept Ciencias Exatas, Jaboticabal, SP, BrazilPRDTA Ctr Sul APTA, Piracicaba, SP, BrazilUniv Estadual Paulista, Fac Ciencias Agr Vet, Dept Ciencias Exatas, Jaboticabal, SP, BrazilFAPESP: 08/56146-5Funpec-editoraCtr Avancado Pesquisa Tecnol Agronegocio Cana IACUniversidade Estadual Paulista (Unesp)Agência Paulista de Tecnologia dos Agronegócios (APTA)Xavier, M. A.Pinto, L. R.Favero, T. M.Perecin, D. [UNESP]Carlini-Garcia, L. A.Landell, M. G. A.2014-12-03T13:10:35Z2014-12-03T13:10:35Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2268-2277application/pdfhttp://dx.doi.org/10.4238/2014.March.31.7Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 13, n. 1, p. 2268-2277, 2014.1676-5680http://hdl.handle.net/11449/11227910.4238/2014.March.31.7WOS:000334114800152WOS000334114800152.pdf7087372884726559Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2024-06-06T13:43:43Zoai:repositorio.unesp.br:11449/112279Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:29:10.776568Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Paternity identification in sugarcane polycrosses by using microsatellite markers
title Paternity identification in sugarcane polycrosses by using microsatellite markers
spellingShingle Paternity identification in sugarcane polycrosses by using microsatellite markers
Xavier, M. A.
Progenies
Sugarcane breeding
Genetic dissimilarity
title_short Paternity identification in sugarcane polycrosses by using microsatellite markers
title_full Paternity identification in sugarcane polycrosses by using microsatellite markers
title_fullStr Paternity identification in sugarcane polycrosses by using microsatellite markers
title_full_unstemmed Paternity identification in sugarcane polycrosses by using microsatellite markers
title_sort Paternity identification in sugarcane polycrosses by using microsatellite markers
author Xavier, M. A.
author_facet Xavier, M. A.
Pinto, L. R.
Favero, T. M.
Perecin, D. [UNESP]
Carlini-Garcia, L. A.
Landell, M. G. A.
author_role author
author2 Pinto, L. R.
Favero, T. M.
Perecin, D. [UNESP]
Carlini-Garcia, L. A.
Landell, M. G. A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ctr Avancado Pesquisa Tecnol Agronegocio Cana IAC
Universidade Estadual Paulista (Unesp)
Agência Paulista de Tecnologia dos Agronegócios (APTA)
dc.contributor.author.fl_str_mv Xavier, M. A.
Pinto, L. R.
Favero, T. M.
Perecin, D. [UNESP]
Carlini-Garcia, L. A.
Landell, M. G. A.
dc.subject.por.fl_str_mv Progenies
Sugarcane breeding
Genetic dissimilarity
topic Progenies
Sugarcane breeding
Genetic dissimilarity
description Although polycrosses have been used to test the potential of cross-combination of a large number of sugarcane parents, the male parent of the half-sib progenies produced is unknown. The present study aimed to integrate the molecular marker technology to the sugarcane polycross approach by the application of microsatellite markers to identify the male parent of 41 elite clones derived from polycross families. Ten microsatellite [single sequence repeats (SSRs)] primer pairs were used to identify the most likely male parent considering markers present in the selected clone but absent in the female parent. The number of alleles generated by the 10 microsatellite primer pairs ranged from 102 (cross-pollination lantern 4) to 120 (cross-pollination lantern 2) with an average of 113.25 alleles per SSR. The average genetic similarity among the involved parents in the polycrosses was 45.9%. The results of the analysis of the SSR markers absent in the female parent and present only in the selected clone as well as the genetic similarity values allowed the identification of the most likely male parent in 73% of the total clones evaluated and also to detect probable contaminations. The obtained results highlight the importance of using molecular marker technology in the identification and confirmation of the male parent of high-performance clones derived from polycrosses in the sugarcane breeding programs.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-03T13:10:35Z
2014-12-03T13:10:35Z
2014-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4238/2014.March.31.7
Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 13, n. 1, p. 2268-2277, 2014.
1676-5680
http://hdl.handle.net/11449/112279
10.4238/2014.March.31.7
WOS:000334114800152
WOS000334114800152.pdf
7087372884726559
url http://dx.doi.org/10.4238/2014.March.31.7
http://hdl.handle.net/11449/112279
identifier_str_mv Genetics And Molecular Research. Ribeirao Preto: Funpec-editora, v. 13, n. 1, p. 2268-2277, 2014.
1676-5680
10.4238/2014.March.31.7
WOS:000334114800152
WOS000334114800152.pdf
7087372884726559
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Research
0,439
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2268-2277
application/pdf
dc.publisher.none.fl_str_mv Funpec-editora
publisher.none.fl_str_mv Funpec-editora
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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