Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biopha.2018.01.022 http://hdl.handle.net/11449/179500 |
Resumo: | Brazilian Northeast is the world's largest producer of Agave sisalana Perrine for the supply of the sisal fiber. About 95% of plant biomass, which comprise the mucilage and sisal juice, is considered a waste residual is discarded in the soil. However, the sisal juice is rich in steroidal saponins, which exhibits different pharmacological properties. Despite this, natural products are not necessarily safe. Based on this, this study analyzed the antioxidant, cytotoxic and mutagenic potential of three extracts derived from acid hydrolysis (AHAS), dried precipitate (DPAS) and hexanic of A. sisalana (HAS). These analyses were performed by in vitro and in vivo methods, using Vero cells, human lymphocytes and mice. Results showed that AHAS 50 and 100 can be considered a useful antineoplastic candidate due to their antioxidant and cytotoxic activity, with no genotoxic/clastogenic potential in Vero cells and mice. Although the comet assay in human lymphocytes has showed that the AHAS 25, AHAS 50 and AHAS 100 can lead to DNA breaks, these extracts did not promote DNA damages in mice bone marrow. Considering the different mutagenic responses obtained with the different methods employed, this study suggest that the metabolizing pathways can produce by-products harmful to health. For this reason, it is mandatory to analyze the mutagenic potential by both in vitro and in vivo techniques, using cells derived from different species and origins. |
id |
UNSP_9d26bf3b93602170625d2e6fb68d4674 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/179500 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytesAgave sisalanaComet assayHistone γ-H2AX assayMicronucleus testMutagenesisBrazilian Northeast is the world's largest producer of Agave sisalana Perrine for the supply of the sisal fiber. About 95% of plant biomass, which comprise the mucilage and sisal juice, is considered a waste residual is discarded in the soil. However, the sisal juice is rich in steroidal saponins, which exhibits different pharmacological properties. Despite this, natural products are not necessarily safe. Based on this, this study analyzed the antioxidant, cytotoxic and mutagenic potential of three extracts derived from acid hydrolysis (AHAS), dried precipitate (DPAS) and hexanic of A. sisalana (HAS). These analyses were performed by in vitro and in vivo methods, using Vero cells, human lymphocytes and mice. Results showed that AHAS 50 and 100 can be considered a useful antineoplastic candidate due to their antioxidant and cytotoxic activity, with no genotoxic/clastogenic potential in Vero cells and mice. Although the comet assay in human lymphocytes has showed that the AHAS 25, AHAS 50 and AHAS 100 can lead to DNA breaks, these extracts did not promote DNA damages in mice bone marrow. Considering the different mutagenic responses obtained with the different methods employed, this study suggest that the metabolizing pathways can produce by-products harmful to health. For this reason, it is mandatory to analyze the mutagenic potential by both in vitro and in vivo techniques, using cells derived from different species and origins.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Genetics Laboratory Butantan InstituteBiotechnology Interunit Post-Graduation Program Biomedical Science Institute University of São Paulo (USP)Department of Biotechnology School of Science Humanities and Languages São Paulo State University (UNESP), AssisCenter of Toxins Immune-Response and Cell Signaling (CeTICS/CEPID) Butantan InstituteDepartment of Biotechnology School of Science Humanities and Languages São Paulo State University (UNESP), AssisFAPESP: 2014/20617-5Butantan InstituteUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Araldi, Rodrigo Pinheirodos Santos, Maristela Oliveira [UNESP]Barbon, Fabiane Faria [UNESP]Manjerona, Bruna Aparecida [UNESP]Meirelles, Bruno Rosa [UNESP]de Oliva Neto, Pedro [UNESP]da Silva, Pedro Ismaeldos Santos, Lucinéia [UNESP]Camargo, Isabel Cristina Cherici [UNESP]de Souza, Edislane Barreiros [UNESP]2018-12-11T17:35:26Z2018-12-11T17:35:26Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article873-885http://dx.doi.org/10.1016/j.biopha.2018.01.022Biomedicine and Pharmacotherapy, v. 98, p. 873-885.1950-60070753-3322http://hdl.handle.net/11449/17950010.1016/j.biopha.2018.01.0222-s2.0-850403421002-s2.0-85040342100.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomedicine and Pharmacotherapy0,951info:eu-repo/semantics/openAccess2024-06-13T17:38:43Zoai:repositorio.unesp.br:11449/179500Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:19:46.527495Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes |
title |
Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes |
spellingShingle |
Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes Araldi, Rodrigo Pinheiro Agave sisalana Comet assay Histone γ-H2AX assay Micronucleus test Mutagenesis |
title_short |
Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes |
title_full |
Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes |
title_fullStr |
Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes |
title_full_unstemmed |
Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes |
title_sort |
Analysis of antioxidant, cytotoxic and mutagenic potential of Agave sisalana Perrine extracts using Vero cells, human lymphocytes and mice polychromatic erythrocytes |
author |
Araldi, Rodrigo Pinheiro |
author_facet |
Araldi, Rodrigo Pinheiro dos Santos, Maristela Oliveira [UNESP] Barbon, Fabiane Faria [UNESP] Manjerona, Bruna Aparecida [UNESP] Meirelles, Bruno Rosa [UNESP] de Oliva Neto, Pedro [UNESP] da Silva, Pedro Ismael dos Santos, Lucinéia [UNESP] Camargo, Isabel Cristina Cherici [UNESP] de Souza, Edislane Barreiros [UNESP] |
author_role |
author |
author2 |
dos Santos, Maristela Oliveira [UNESP] Barbon, Fabiane Faria [UNESP] Manjerona, Bruna Aparecida [UNESP] Meirelles, Bruno Rosa [UNESP] de Oliva Neto, Pedro [UNESP] da Silva, Pedro Ismael dos Santos, Lucinéia [UNESP] Camargo, Isabel Cristina Cherici [UNESP] de Souza, Edislane Barreiros [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Butantan Institute Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Araldi, Rodrigo Pinheiro dos Santos, Maristela Oliveira [UNESP] Barbon, Fabiane Faria [UNESP] Manjerona, Bruna Aparecida [UNESP] Meirelles, Bruno Rosa [UNESP] de Oliva Neto, Pedro [UNESP] da Silva, Pedro Ismael dos Santos, Lucinéia [UNESP] Camargo, Isabel Cristina Cherici [UNESP] de Souza, Edislane Barreiros [UNESP] |
dc.subject.por.fl_str_mv |
Agave sisalana Comet assay Histone γ-H2AX assay Micronucleus test Mutagenesis |
topic |
Agave sisalana Comet assay Histone γ-H2AX assay Micronucleus test Mutagenesis |
description |
Brazilian Northeast is the world's largest producer of Agave sisalana Perrine for the supply of the sisal fiber. About 95% of plant biomass, which comprise the mucilage and sisal juice, is considered a waste residual is discarded in the soil. However, the sisal juice is rich in steroidal saponins, which exhibits different pharmacological properties. Despite this, natural products are not necessarily safe. Based on this, this study analyzed the antioxidant, cytotoxic and mutagenic potential of three extracts derived from acid hydrolysis (AHAS), dried precipitate (DPAS) and hexanic of A. sisalana (HAS). These analyses were performed by in vitro and in vivo methods, using Vero cells, human lymphocytes and mice. Results showed that AHAS 50 and 100 can be considered a useful antineoplastic candidate due to their antioxidant and cytotoxic activity, with no genotoxic/clastogenic potential in Vero cells and mice. Although the comet assay in human lymphocytes has showed that the AHAS 25, AHAS 50 and AHAS 100 can lead to DNA breaks, these extracts did not promote DNA damages in mice bone marrow. Considering the different mutagenic responses obtained with the different methods employed, this study suggest that the metabolizing pathways can produce by-products harmful to health. For this reason, it is mandatory to analyze the mutagenic potential by both in vitro and in vivo techniques, using cells derived from different species and origins. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:35:26Z 2018-12-11T17:35:26Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biopha.2018.01.022 Biomedicine and Pharmacotherapy, v. 98, p. 873-885. 1950-6007 0753-3322 http://hdl.handle.net/11449/179500 10.1016/j.biopha.2018.01.022 2-s2.0-85040342100 2-s2.0-85040342100.pdf |
url |
http://dx.doi.org/10.1016/j.biopha.2018.01.022 http://hdl.handle.net/11449/179500 |
identifier_str_mv |
Biomedicine and Pharmacotherapy, v. 98, p. 873-885. 1950-6007 0753-3322 10.1016/j.biopha.2018.01.022 2-s2.0-85040342100 2-s2.0-85040342100.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomedicine and Pharmacotherapy 0,951 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
873-885 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129310144331776 |