First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil

Detalhes bibliográficos
Autor(a) principal: Inácio, Sandra Valéria [UNESP]
Data de Publicação: 2017
Outros Autores: Zucatto, Anaiza Simão [UNESP], de Aquino, Monally Conceição Costa [UNESP], Oliveira, Bruno César Miranda [UNESP], Bresciani, Katia Denise Saraiva [UNESP], Widmer, Giovanni, de Brito, Roberta Lomonte Lemos [UNESP], Neto, Luiz da Silveira [UNESP], Nakamura, Alex Akira [UNESP], Meireles, Marcelo Vasconcelos [UNESP], Carvalho, João Gabriel Balizardo [UNESP], Gomes, Jancarlo Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetpar.2016.11.021
http://hdl.handle.net/11449/173989
Resumo: The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from five fecal samples. PCR amplification and sequencing of a fragment of the GP60 sporozoite surface glycoprotein gene revealed C. parvum genotypes IIaA18G3R1, IIaA15G2R1. Interestingly, we also detected in two foals a GP60 genotype related to the human parasite C. hominis.
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spelling First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in BrazilEquineMolecular biologyParasiteThe present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from five fecal samples. PCR amplification and sequencing of a fragment of the GP60 sporozoite surface glycoprotein gene revealed C. parvum genotypes IIaA18G3R1, IIaA15G2R1. Interestingly, we also detected in two foals a GP60 genotype related to the human parasite C. hominis.Universidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária Araçatuba Departamento de Apoio Produção e Saúde AnimalDepartment of Infectious Disease & Global Health Cummings School of Veterinary Medicine at Tufts UniversityUniversidade Estadual Paulista (Unesp) de Ciências Agrárias e Veterinárias Departamento de Medicina Veterinária Preventiva e Reprodução AnimalUniversidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária Araçatuba Departamento de Clínica Cirurgia e Reprodução AnimalUniversidade Estadual Paulista (Unesp), Faculdade de Medicina VeterináriaUniversidade Estadual de Campinas (Unicamp), Institutos de Biologia e Computação, CampinasUniversidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária Araçatuba Departamento de Apoio Produção e Saúde AnimalUniversidade Estadual Paulista (Unesp) de Ciências Agrárias e Veterinárias Departamento de Medicina Veterinária Preventiva e Reprodução AnimalUniversidade Estadual Paulista (Unesp) Faculdade de Medicina Veterinária Araçatuba Departamento de Clínica Cirurgia e Reprodução AnimalUniversidade Estadual Paulista (Unesp), Faculdade de Medicina VeterináriaUniversidade Estadual Paulista (Unesp)Cummings School of Veterinary Medicine at Tufts UniversityUniversidade Estadual de Campinas (UNICAMP)Inácio, Sandra Valéria [UNESP]Zucatto, Anaiza Simão [UNESP]de Aquino, Monally Conceição Costa [UNESP]Oliveira, Bruno César Miranda [UNESP]Bresciani, Katia Denise Saraiva [UNESP]Widmer, Giovannide Brito, Roberta Lomonte Lemos [UNESP]Neto, Luiz da Silveira [UNESP]Nakamura, Alex Akira [UNESP]Meireles, Marcelo Vasconcelos [UNESP]Carvalho, João Gabriel Balizardo [UNESP]Gomes, Jancarlo Ferreira2018-12-11T17:08:38Z2018-12-11T17:08:38Z2017-01-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article48-51application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2016.11.021Veterinary Parasitology, v. 233, p. 48-51.1873-25500304-4017http://hdl.handle.net/11449/17398910.1016/j.vetpar.2016.11.0212-s2.0-850073045282-s2.0-85007304528.pdf59505943668296470903513897615274Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology1,275info:eu-repo/semantics/openAccess2024-09-04T19:15:25Zoai:repositorio.unesp.br:11449/173989Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil
title First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil
spellingShingle First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil
Inácio, Sandra Valéria [UNESP]
Equine
Molecular biology
Parasite
title_short First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil
title_full First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil
title_fullStr First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil
title_full_unstemmed First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil
title_sort First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil
author Inácio, Sandra Valéria [UNESP]
author_facet Inácio, Sandra Valéria [UNESP]
Zucatto, Anaiza Simão [UNESP]
de Aquino, Monally Conceição Costa [UNESP]
Oliveira, Bruno César Miranda [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
Widmer, Giovanni
de Brito, Roberta Lomonte Lemos [UNESP]
Neto, Luiz da Silveira [UNESP]
Nakamura, Alex Akira [UNESP]
Meireles, Marcelo Vasconcelos [UNESP]
Carvalho, João Gabriel Balizardo [UNESP]
Gomes, Jancarlo Ferreira
author_role author
author2 Zucatto, Anaiza Simão [UNESP]
de Aquino, Monally Conceição Costa [UNESP]
Oliveira, Bruno César Miranda [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
Widmer, Giovanni
de Brito, Roberta Lomonte Lemos [UNESP]
Neto, Luiz da Silveira [UNESP]
Nakamura, Alex Akira [UNESP]
Meireles, Marcelo Vasconcelos [UNESP]
Carvalho, João Gabriel Balizardo [UNESP]
Gomes, Jancarlo Ferreira
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Cummings School of Veterinary Medicine at Tufts University
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Inácio, Sandra Valéria [UNESP]
Zucatto, Anaiza Simão [UNESP]
de Aquino, Monally Conceição Costa [UNESP]
Oliveira, Bruno César Miranda [UNESP]
Bresciani, Katia Denise Saraiva [UNESP]
Widmer, Giovanni
de Brito, Roberta Lomonte Lemos [UNESP]
Neto, Luiz da Silveira [UNESP]
Nakamura, Alex Akira [UNESP]
Meireles, Marcelo Vasconcelos [UNESP]
Carvalho, João Gabriel Balizardo [UNESP]
Gomes, Jancarlo Ferreira
dc.subject.por.fl_str_mv Equine
Molecular biology
Parasite
topic Equine
Molecular biology
Parasite
description The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from five fecal samples. PCR amplification and sequencing of a fragment of the GP60 sporozoite surface glycoprotein gene revealed C. parvum genotypes IIaA18G3R1, IIaA15G2R1. Interestingly, we also detected in two foals a GP60 genotype related to the human parasite C. hominis.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-15
2018-12-11T17:08:38Z
2018-12-11T17:08:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2016.11.021
Veterinary Parasitology, v. 233, p. 48-51.
1873-2550
0304-4017
http://hdl.handle.net/11449/173989
10.1016/j.vetpar.2016.11.021
2-s2.0-85007304528
2-s2.0-85007304528.pdf
5950594366829647
0903513897615274
url http://dx.doi.org/10.1016/j.vetpar.2016.11.021
http://hdl.handle.net/11449/173989
identifier_str_mv Veterinary Parasitology, v. 233, p. 48-51.
1873-2550
0304-4017
10.1016/j.vetpar.2016.11.021
2-s2.0-85007304528
2-s2.0-85007304528.pdf
5950594366829647
0903513897615274
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Parasitology
1,275
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 48-51
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021381630328832

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