Resposta tecidual a tubos de dentina irrigados com dispersão de nanopartículas de prata a 47ppm ou hipoclorito de sódio a 2,5%

• Autor(a) principal: Dalto, Luana Godoy [UNESP]
• Data de Publicação: 2011
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149402; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-26/000846824.pdf
• topic: Teste de materiais; Nanopartículas; Hipoclorito de sódio; Materials Testing

The aim of this study was to evaluate the tissue response to implanted dentine tubes irrigated with silver nanoparticles dispersion or 2.5% sodium hyppochlorite. Thirty rats will individually receive two dentine tubes irrigated with 47ppm silver nanoparticles dispersion, 2.5% sodium hypochlorite or saline as controle. The observation periods will be 7 and 30 days. After each period of time, fifteen animals will be killed and the tubes and surrounding tissue removed, fixed and prepared to be analyzed in light microscope with glycol methacrylate embedding, 3 m serial cutting and HE staining. Scores will be defined as follows: 0, none or few inflammatory cells, no reaction; 1, <25 cells, mild reaction; 2, 25 to 125 cells, moderate reaction; 3, >125 cells, severe reaction. Fibrous capsule was categorized as thin when thickness was < 150 μm and thick at > 150 μm. Necrosis and formation of calcification were recorded. The data will be statistically analyzed using Kruskal Wallis test. All groups showed a thin fibrous capsule and mild inflammatory infiltrate specially on the 30th day. This study concluded that the dispersion of silver nanoparticles was biocompatible when compared with sodium hypochlorite solution, but further studies are needed to better analyze the properties of this material and confirm the data observed