Resposta tecidual a tubos de dentina contaminados e irrigados com nanopartícula de prata na concentração de 23ppm ou hipoclorito de sódio a 2,5%

• Autor(a) principal: Pacanaro, Sara Vieira [UNESP]
• Data de Publicação: 2012
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149463; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-27/000855813.pdf
• topic: Biocompatibilidade; Nanopartículas; Hipoclorito de sódio; Nanoparticles

The aim of this study was to evaluate the tecidual answer effect of silver nanoparticles dispersion using the tissue response to implanted dentine tubes irrigated with silver nanoparticles dispersion or 2.5% sodium hyppochlorite. Forty rats was individually receive two dentine tubes irrigated with 23ppm silver nanoparticles dispersion, 2.5% sodium hypochlorite, saline or sterilized tube as control. The observation periods was 7 and 30 days. After each period of time, twenty animals was killed and the tubes and surrounding tissue removed, fixed and prepared to be analyzed in light microscope with glycol methacrylate embedding, 3 m serial cutting and HE staining. Scores will be defined as follows: 0, none or few inflammatory cells, no reaction; 1, <25 cells, mild reaction; 2, 25 to 125 cells, moderate reaction; 3, >125 cells, severe reaction. Fibrous capsule was categorized as thin when thickness was < 150 μm and thick at > 150 μm. Necrosis and formation of calcification were recorded.. The data were statistically analyzed using Kruskal Wallis test. At 7th day, all groups had a mild inflammatory infiltrate and thick fibrotic capsule. The intensity of inflammation reduced on the 30th day, all groups, and a thin fibrous capsule was found. This study concluded that the dispersion of silver nanoparticles was biocompatible when compared with sodium hypochlorite solution, but further studies are needed to better analyze the properties of this material and confirm the data observed