Spontaneous canine transmissible venereal tumor: Association between different phenotypes and the insertion LINE-1/c-myc

• Autor(a) principal: Fonseca, Luciano S. [UNESP]
• Data de Publicação: 2012
• Outros Autores: Mota, Lígia S.L.S. [UNESP], Colodel, Marcia M. [UNESP], Ferreira, Isabelle [UNESP], Brandão, Cláudia Valéria S [UNESP], Rocha, Noeme S. [UNESP]
• Tipo de documento: Artigo
• Idioma: eng; spa
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/226981
• topic: Dogs; Oncogenes; Polymerase Chain Reaction; Transposition elements

Objective: This study aimed to evaluate the LINE-1 transposon inserted in c-myc gene as a specific genetic alteration in cells of spontaneous canine Transmissible Venereal Tumor (TVT) with either lymphocytoid or plasmacytoid phenotypes. Methods: Tumoral biopsies from 35 dogs were collected by puncture or exfoliation. Polymerase Chain Reaction (PCR) was carried out with primers myc.s and LINE. A, specific to the LINE-1 segment to detect the presence of LINE-1/c-myc molecular marker. Results: Sequence alignment of DNA samples from lymphocytoid and plasmacytoid TVT cells did not show polymorphisms, and the comparison with sequences from the GenBank identified them as a LINE-1/c-myc rearrangement. Conclusions: Considering the aggressive nature of the plasmacytoid phenotype, there is no apparent relation between LINE-1/c-myc and the malignancy of TVT. Further studies are needed to establish molecular differences associated with the aggressiveness of the various phenotypes of canine TVT.