Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-myc

Detalhes bibliográficos
Autor(a) principal: Fonseca, Luciano S. [UNESP]
Data de Publicação: 2012
Outros Autores: Mota, Ligia S. L. S. [UNESP], Colodel, Marcia M. [UNESP], Ferreira, Isabelle [UNESP], Brandão, Claudia Valeria Seullner [UNESP], Rocha, Noeme Sousa [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902012000300007&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/11156
Resumo: Objective: this study aimed to evaluate the LINE-1 transposon inserted in c-myc gene as a specific genetic alteration in cells of spontaneous canine Transmissible Venereal Tumor (TVT) with either lymphocytoid or plasmacytoid phenotypes. Methods: tumoral biopsies from 35 dogs were collected by puncture or exfoliation. Polymerase Chain Reaction (PCR) was carried out with primers myc.s and LINE. A, specific to the LINE-1 segment to detect the presence of LINE-1/c-myc molecular marker. Results: sequence alignment of DNA samples from lymphocytoid and plasmacytoid TVT cells did not show polymorphisms, and the comparison with sequences from the GenBank identified them as a LINE-1/c-myc rearrangement. Conclusions: considering the aggressive nature of the plasmacytoid phenotype, there is no apparent relation between LINE-1/c-myc and the malignancy of TVT. Further studies are needed to establish molecular differences associated with the aggressiveness of the various phenotypes of canine TVT.
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spelling Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-mycTumor venéreo transmisible canino espontáneo: asociación entre diferentes fenotipos y la inserción LINE -1/c-mycTumor venéreo transmissível canino espontâneo: associação entre diferentes fenótipos e a inserção LINE-1/c-mycdogsoncogenesPolymerase Chain Reactiontransposition elementsObjective: this study aimed to evaluate the LINE-1 transposon inserted in c-myc gene as a specific genetic alteration in cells of spontaneous canine Transmissible Venereal Tumor (TVT) with either lymphocytoid or plasmacytoid phenotypes. Methods: tumoral biopsies from 35 dogs were collected by puncture or exfoliation. Polymerase Chain Reaction (PCR) was carried out with primers myc.s and LINE. A, specific to the LINE-1 segment to detect the presence of LINE-1/c-myc molecular marker. Results: sequence alignment of DNA samples from lymphocytoid and plasmacytoid TVT cells did not show polymorphisms, and the comparison with sequences from the GenBank identified them as a LINE-1/c-myc rearrangement. Conclusions: considering the aggressive nature of the plasmacytoid phenotype, there is no apparent relation between LINE-1/c-myc and the malignancy of TVT. Further studies are needed to establish molecular differences associated with the aggressiveness of the various phenotypes of canine TVT.Objetivo: evaluar la expresión del trasposón LINE-1 insertado en el oncogén c-myc como una alteración genética específica en células de Tumor Venéreo Transmisible canino espontáneo con fenotipos linfocitoideos o plasmocitoideos. Métodos: se tomaron muestras citológicas de 35 caninos naturalmente afectados por Tumor Venéreo Transmisible (TVT), separándolas en dos grupos de acuerdo al fenotipo predominante. La identificación del marcador molecular LINE-1/c-myc fue posible mediante la técnica de Reacción en Cadena de la Polimerasa (PCR) usando los primers myc.s y LINE. A, específicos para el segmento LINE-1. Resultados: el resultado del alineamiento de las secuencias obtenidas a partir del DNA de cada uno de los fenotipos de TVT no presentó variación entre ellos y al compararlas con el alineamiento de otras secuencias depositadas en el GenBank, pudo observarse que se trata de un reordenamiento LINE- 1/c-myc. Conclusiones: teniendo en cuenta la naturaleza agresiva del fenotipo plasmocitoide, se estableció que no hay polimorfismo genético entre los grupos analizados, siendo necesario realizar nuevos estudios tendientes a establecer diferencias moleculares asociadas con la agresividad de los diferentesObjetivo: foi avaliado o elemento de transposição LINE-1 inserido no gene c-myc como alteração genética específica nas células do TVT espontâneo canino nos grupos fenotípicos previamente classificados como linfocitóide e plasmocitóide. Métodos: amostras da lesão de 35 cães foram colhidas por punção ou esfoliação. Para identificar o marcador molecular LINE-1/c-myc nas amostras foi utilizada a técnica de Reação em Cadeia da Polimerase empregando-se os primers myc.s e LINE. A, específicos para o segmento LINE-1. Resultados: o resultado do alinhamento das sequências obtidas das amostras de DNA das células de TVT linfocitóide e plasmocitóide não apresentou polimorfismos e, por meio do alinhamento com outras sequências depositadas no GeneBank, identificou-se que trata-se do rearranjo LINE-1/c-myc. Conclusões: parece não haver relação entre este aspecto molecular analisado com a agressividade do tumor.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP, Dept Clin Vet Med, FMVZ, Botucatu, SP, BrazilUNESP, Dept Genet, Inst Biosci, Botucatu, SP, BrazilUNESP, FMVZ, Dept Surg & Vet Anesthesiol, Botucatu, SP, BrazilUNESP, Dept Clin Vet Med, FMVZ, Botucatu, SP, BrazilUNESP, Dept Genet, Inst Biosci, Botucatu, SP, BrazilUNESP, FMVZ, Dept Surg & Vet Anesthesiol, Botucatu, SP, BrazilUniv Antioquia, Fac Ciencias AgrariasUniversidade Estadual Paulista (Unesp)Fonseca, Luciano S. [UNESP]Mota, Ligia S. L. S. [UNESP]Colodel, Marcia M. [UNESP]Ferreira, Isabelle [UNESP]Brandão, Claudia Valeria Seullner [UNESP]Rocha, Noeme Sousa [UNESP]2014-05-20T13:32:40Z2014-05-20T13:32:40Z2012-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article402-408application/pdfhttp://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902012000300007&lng=en&nrm=iso&tlng=enRevista Colombiana de Ciências Pecuarias. Medellin: Univ Antioquia, Fac Ciencias Agrarias, v. 25, n. 3, p. 402-408, 2012.0120-0690http://hdl.handle.net/11449/11156S0120-06902012000300007WOS:000311716600007S0120-06902012000300007-en.pdf607773591846928410876153890136550000-0002-8188-81490000-0002-2011-5214Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Colombiana de Ciências Pecuarias0.5160,192info:eu-repo/semantics/openAccess2024-08-14T13:20:25Zoai:repositorio.unesp.br:11449/11156Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T13:20:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-myc
Tumor venéreo transmisible canino espontáneo: asociación entre diferentes fenotipos y la inserción LINE -1/c-myc
Tumor venéreo transmissível canino espontâneo: associação entre diferentes fenótipos e a inserção LINE-1/c-myc
title Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-myc
spellingShingle Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-myc
Fonseca, Luciano S. [UNESP]
dogs
oncogenes
Polymerase Chain Reaction
transposition elements
title_short Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-myc
title_full Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-myc
title_fullStr Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-myc
title_full_unstemmed Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-myc
title_sort Spontaneous canine transmissible venereal tumor: association between different phenotypes and the insertion LINE-1/c-myc
author Fonseca, Luciano S. [UNESP]
author_facet Fonseca, Luciano S. [UNESP]
Mota, Ligia S. L. S. [UNESP]
Colodel, Marcia M. [UNESP]
Ferreira, Isabelle [UNESP]
Brandão, Claudia Valeria Seullner [UNESP]
Rocha, Noeme Sousa [UNESP]
author_role author
author2 Mota, Ligia S. L. S. [UNESP]
Colodel, Marcia M. [UNESP]
Ferreira, Isabelle [UNESP]
Brandão, Claudia Valeria Seullner [UNESP]
Rocha, Noeme Sousa [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Fonseca, Luciano S. [UNESP]
Mota, Ligia S. L. S. [UNESP]
Colodel, Marcia M. [UNESP]
Ferreira, Isabelle [UNESP]
Brandão, Claudia Valeria Seullner [UNESP]
Rocha, Noeme Sousa [UNESP]
dc.subject.por.fl_str_mv dogs
oncogenes
Polymerase Chain Reaction
transposition elements
topic dogs
oncogenes
Polymerase Chain Reaction
transposition elements
description Objective: this study aimed to evaluate the LINE-1 transposon inserted in c-myc gene as a specific genetic alteration in cells of spontaneous canine Transmissible Venereal Tumor (TVT) with either lymphocytoid or plasmacytoid phenotypes. Methods: tumoral biopsies from 35 dogs were collected by puncture or exfoliation. Polymerase Chain Reaction (PCR) was carried out with primers myc.s and LINE. A, specific to the LINE-1 segment to detect the presence of LINE-1/c-myc molecular marker. Results: sequence alignment of DNA samples from lymphocytoid and plasmacytoid TVT cells did not show polymorphisms, and the comparison with sequences from the GenBank identified them as a LINE-1/c-myc rearrangement. Conclusions: considering the aggressive nature of the plasmacytoid phenotype, there is no apparent relation between LINE-1/c-myc and the malignancy of TVT. Further studies are needed to establish molecular differences associated with the aggressiveness of the various phenotypes of canine TVT.
publishDate 2012
dc.date.none.fl_str_mv 2012-07-01
2014-05-20T13:32:40Z
2014-05-20T13:32:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902012000300007&lng=en&nrm=iso&tlng=en
Revista Colombiana de Ciências Pecuarias. Medellin: Univ Antioquia, Fac Ciencias Agrarias, v. 25, n. 3, p. 402-408, 2012.
0120-0690
http://hdl.handle.net/11449/11156
S0120-06902012000300007
WOS:000311716600007
S0120-06902012000300007-en.pdf
6077735918469284
1087615389013655
0000-0002-8188-8149
0000-0002-2011-5214
url http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902012000300007&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/11156
identifier_str_mv Revista Colombiana de Ciências Pecuarias. Medellin: Univ Antioquia, Fac Ciencias Agrarias, v. 25, n. 3, p. 402-408, 2012.
0120-0690
S0120-06902012000300007
WOS:000311716600007
S0120-06902012000300007-en.pdf
6077735918469284
1087615389013655
0000-0002-8188-8149
0000-0002-2011-5214
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Colombiana de Ciências Pecuarias
0.516
0,192
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 402-408
application/pdf
dc.publisher.none.fl_str_mv Univ Antioquia, Fac Ciencias Agrarias
publisher.none.fl_str_mv Univ Antioquia, Fac Ciencias Agrarias
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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