The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph

Detalhes bibliográficos
Autor(a) principal: Gregório, Elisa A. [UNESP]
Data de Publicação: 1991
Outros Autores: Ratcliffe, Norman A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02761991000200007
http://hdl.handle.net/11449/18457
Resumo: Haemolymph, heads, salivary glands, crops, midguts, hindguts, and Malpighian tubules from Rhodnius prolixus and Triatoma infestans were extracted in phosphate or Tris buffer saline with calcium, and tested for agglutination and lytic activities by microtitration against both vertebrateerythrocytes and cultured epimatigote forms of Trypanosoma rangeli. Haemagglutination activity against rabbit erythrocytes was found in the crop, midgut and hindgut extracts of T. infestans but only in the haemolymph of R. prolixus. Higher titres of parasite agglutinins were found in R. prolixus haemolymph than T. infestans, whilst the converse occurred for the tissue extracts. In addition, the extracts of T. infestans salivary glands, but not those of R. prolixus, showed a trypanolytic activity that was heat-inactivated and was not abolished by pre-incubation with any of the sugars or glycoproteins tested. T. infestans, which is refractory to infection by T. rangeli, thus appears to contain a much wider distribution of agglutinating and trypanolytic factors in its tissues than the more susceptible species, R. prolixus
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spelling The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymphhaemagglutininRhodnius prolixusTriatoma infestanshaemolymphtrypanolytic factorTrypanosoma rangeliHaemolymph, heads, salivary glands, crops, midguts, hindguts, and Malpighian tubules from Rhodnius prolixus and Triatoma infestans were extracted in phosphate or Tris buffer saline with calcium, and tested for agglutination and lytic activities by microtitration against both vertebrateerythrocytes and cultured epimatigote forms of Trypanosoma rangeli. Haemagglutination activity against rabbit erythrocytes was found in the crop, midgut and hindgut extracts of T. infestans but only in the haemolymph of R. prolixus. Higher titres of parasite agglutinins were found in R. prolixus haemolymph than T. infestans, whilst the converse occurred for the tissue extracts. In addition, the extracts of T. infestans salivary glands, but not those of R. prolixus, showed a trypanolytic activity that was heat-inactivated and was not abolished by pre-incubation with any of the sugars or glycoproteins tested. T. infestans, which is refractory to infection by T. rangeli, thus appears to contain a much wider distribution of agglutinating and trypanolytic factors in its tissues than the more susceptible species, R. prolixusUNESP Instituto de BiociênciasUniversity College of Swansea School of Biological Sciences Biomedical & Physiological Research GroupUNESP Instituto de BiociênciasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual Paulista (Unesp)University College of Swansea School of Biological Sciences Biomedical & Physiological Research GroupGregório, Elisa A. [UNESP]Ratcliffe, Norman A.2014-05-20T13:51:36Z2014-05-20T13:51:36Z1991-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article181-186application/pdfhttp://dx.doi.org/10.1590/S0074-02761991000200007Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 86, n. 2, p. 181-186, 1991.0074-0276http://hdl.handle.net/11449/1845710.1590/S0074-02761991000200007S0074-02761991000200007S0074-02761991000200007.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-10-18T06:06:45Zoai:repositorio.unesp.br:11449/18457Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:14:39.770113Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph
title The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph
spellingShingle The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph
Gregório, Elisa A. [UNESP]
haemagglutinin
Rhodnius prolixus
Triatoma infestans
haemolymph
trypanolytic factor
Trypanosoma rangeli
title_short The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph
title_full The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph
title_fullStr The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph
title_full_unstemmed The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph
title_sort The distribution of agglutinins and lytic activity against Trypanosoma rangeli and erythrocytes in Rhodnius prolixus and Triatoma infestans tissue extracts and haemolymph
author Gregório, Elisa A. [UNESP]
author_facet Gregório, Elisa A. [UNESP]
Ratcliffe, Norman A.
author_role author
author2 Ratcliffe, Norman A.
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University College of Swansea School of Biological Sciences Biomedical & Physiological Research Group
dc.contributor.author.fl_str_mv Gregório, Elisa A. [UNESP]
Ratcliffe, Norman A.
dc.subject.por.fl_str_mv haemagglutinin
Rhodnius prolixus
Triatoma infestans
haemolymph
trypanolytic factor
Trypanosoma rangeli
topic haemagglutinin
Rhodnius prolixus
Triatoma infestans
haemolymph
trypanolytic factor
Trypanosoma rangeli
description Haemolymph, heads, salivary glands, crops, midguts, hindguts, and Malpighian tubules from Rhodnius prolixus and Triatoma infestans were extracted in phosphate or Tris buffer saline with calcium, and tested for agglutination and lytic activities by microtitration against both vertebrateerythrocytes and cultured epimatigote forms of Trypanosoma rangeli. Haemagglutination activity against rabbit erythrocytes was found in the crop, midgut and hindgut extracts of T. infestans but only in the haemolymph of R. prolixus. Higher titres of parasite agglutinins were found in R. prolixus haemolymph than T. infestans, whilst the converse occurred for the tissue extracts. In addition, the extracts of T. infestans salivary glands, but not those of R. prolixus, showed a trypanolytic activity that was heat-inactivated and was not abolished by pre-incubation with any of the sugars or glycoproteins tested. T. infestans, which is refractory to infection by T. rangeli, thus appears to contain a much wider distribution of agglutinating and trypanolytic factors in its tissues than the more susceptible species, R. prolixus
publishDate 1991
dc.date.none.fl_str_mv 1991-06-01
2014-05-20T13:51:36Z
2014-05-20T13:51:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02761991000200007
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 86, n. 2, p. 181-186, 1991.
0074-0276
http://hdl.handle.net/11449/18457
10.1590/S0074-02761991000200007
S0074-02761991000200007
S0074-02761991000200007.pdf
url http://dx.doi.org/10.1590/S0074-02761991000200007
http://hdl.handle.net/11449/18457
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 86, n. 2, p. 181-186, 1991.
0074-0276
10.1590/S0074-02761991000200007
S0074-02761991000200007
S0074-02761991000200007.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
2.833
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 181-186
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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