Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria

• Autor(a) principal: Perez Navarro, Miguel Octavio
• Data de Publicação: 2020
• Outros Autores: Dilarri, Guilherme [UNESP], Simionato, Ane Stefano, Grzegorczyk, Kathlen, Dealis, Mickely Liuti, Cano, Barbara Gionco, Barazetti, Andre Riedi, Afonso, Leandro, Chryssafidis, Andreas Lazaros, Ferreira, Henrique [UNESP], Andrade, Galdino
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.3389/fmicb.2020.01076; http://hdl.handle.net/11449/197012
• topic: bactericidal mechanism of action; Xanthomonas; KPC; MRSA; electronic microscopy; fluorescence microscopy

The antibiotic activity of metalloantibiotic compounds has been evaluated since the 90s, and many different modes of action were characterized. In the last decade, the effects of secondary metabolites produced byPseudomonas aeruginosaLV strain, including a cupric compound identified as Fluopsin C, were tested against many pathogenic bacteria strains, proving their high antibiotic activity. In the present study, the bactericidal mechanisms of action of Fluopsin C and the semi-purified fraction F4A were elucidated. The results found in electron microscopy [scanning electron microscopy (SEM) and transmission electronic microscopy (TEM)] demonstrated that both Fluopsin C and F4A are affecting the cytoplasmatic membrane of Gram-positive and Gram-negative bacteria. These results were confirmed by fluorescence microscopy, where these bacteria presented permeabilization of their cytoplasmatic membranes after contact with the semi-purified fraction and pure compound. Using electronic and fluorescence microscopy, along with bacterial mutant strains with marked divisional septum, the membrane was defined as the primary target of Fluopsin C in the tested bacteria.