Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2020.01076 http://hdl.handle.net/11449/197012 |
Resumo: | The antibiotic activity of metalloantibiotic compounds has been evaluated since the 90s, and many different modes of action were characterized. In the last decade, the effects of secondary metabolites produced byPseudomonas aeruginosaLV strain, including a cupric compound identified as Fluopsin C, were tested against many pathogenic bacteria strains, proving their high antibiotic activity. In the present study, the bactericidal mechanisms of action of Fluopsin C and the semi-purified fraction F4A were elucidated. The results found in electron microscopy [scanning electron microscopy (SEM) and transmission electronic microscopy (TEM)] demonstrated that both Fluopsin C and F4A are affecting the cytoplasmatic membrane of Gram-positive and Gram-negative bacteria. These results were confirmed by fluorescence microscopy, where these bacteria presented permeabilization of their cytoplasmatic membranes after contact with the semi-purified fraction and pure compound. Using electronic and fluorescence microscopy, along with bacterial mutant strains with marked divisional septum, the membrane was defined as the primary target of Fluopsin C in the tested bacteria. |
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Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteriabactericidal mechanism of actionXanthomonasKPCMRSAelectronic microscopyfluorescence microscopyThe antibiotic activity of metalloantibiotic compounds has been evaluated since the 90s, and many different modes of action were characterized. In the last decade, the effects of secondary metabolites produced byPseudomonas aeruginosaLV strain, including a cupric compound identified as Fluopsin C, were tested against many pathogenic bacteria strains, proving their high antibiotic activity. In the present study, the bactericidal mechanisms of action of Fluopsin C and the semi-purified fraction F4A were elucidated. The results found in electron microscopy [scanning electron microscopy (SEM) and transmission electronic microscopy (TEM)] demonstrated that both Fluopsin C and F4A are affecting the cytoplasmatic membrane of Gram-positive and Gram-negative bacteria. These results were confirmed by fluorescence microscopy, where these bacteria presented permeabilization of their cytoplasmatic membranes after contact with the semi-purified fraction and pure compound. Using electronic and fluorescence microscopy, along with bacterial mutant strains with marked divisional septum, the membrane was defined as the primary target of Fluopsin C in the tested bacteria.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Araucaria FoundationUniv Estadual Londrina, Dept Microbiol, Microbial Ecol Lab, Londrina, Parana, BrazilUniv Estadual Paulista, Inst Biosci, Dept Biochem & Microbiol, Rio Claro, BrazilUniv Estado Santa Catarina, Ctr Agrovet Sci, Dept Vet Med, Lages, SC, BrazilUniv Estadual Paulista, Inst Biosci, Dept Biochem & Microbiol, Rio Claro, BrazilFrontiers Media SaUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Univ Estado Santa CatarinaPerez Navarro, Miguel OctavioDilarri, Guilherme [UNESP]Simionato, Ane StefanoGrzegorczyk, KathlenDealis, Mickely LiutiCano, Barbara GioncoBarazetti, Andre RiediAfonso, LeandroChryssafidis, Andreas LazarosFerreira, Henrique [UNESP]Andrade, Galdino2020-12-10T20:03:24Z2020-12-10T20:03:24Z2020-06-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.3389/fmicb.2020.01076Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 11, 11 p., 2020.1664-302Xhttp://hdl.handle.net/11449/19701210.3389/fmicb.2020.01076WOS:000543335100001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T10:18:16Zoai:repositorio.unesp.br:11449/197012Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:47:34.692526Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria |
title |
Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria |
spellingShingle |
Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria Perez Navarro, Miguel Octavio bactericidal mechanism of action Xanthomonas KPC MRSA electronic microscopy fluorescence microscopy |
title_short |
Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria |
title_full |
Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria |
title_fullStr |
Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria |
title_full_unstemmed |
Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria |
title_sort |
Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria |
author |
Perez Navarro, Miguel Octavio |
author_facet |
Perez Navarro, Miguel Octavio Dilarri, Guilherme [UNESP] Simionato, Ane Stefano Grzegorczyk, Kathlen Dealis, Mickely Liuti Cano, Barbara Gionco Barazetti, Andre Riedi Afonso, Leandro Chryssafidis, Andreas Lazaros Ferreira, Henrique [UNESP] Andrade, Galdino |
author_role |
author |
author2 |
Dilarri, Guilherme [UNESP] Simionato, Ane Stefano Grzegorczyk, Kathlen Dealis, Mickely Liuti Cano, Barbara Gionco Barazetti, Andre Riedi Afonso, Leandro Chryssafidis, Andreas Lazaros Ferreira, Henrique [UNESP] Andrade, Galdino |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) Univ Estado Santa Catarina |
dc.contributor.author.fl_str_mv |
Perez Navarro, Miguel Octavio Dilarri, Guilherme [UNESP] Simionato, Ane Stefano Grzegorczyk, Kathlen Dealis, Mickely Liuti Cano, Barbara Gionco Barazetti, Andre Riedi Afonso, Leandro Chryssafidis, Andreas Lazaros Ferreira, Henrique [UNESP] Andrade, Galdino |
dc.subject.por.fl_str_mv |
bactericidal mechanism of action Xanthomonas KPC MRSA electronic microscopy fluorescence microscopy |
topic |
bactericidal mechanism of action Xanthomonas KPC MRSA electronic microscopy fluorescence microscopy |
description |
The antibiotic activity of metalloantibiotic compounds has been evaluated since the 90s, and many different modes of action were characterized. In the last decade, the effects of secondary metabolites produced byPseudomonas aeruginosaLV strain, including a cupric compound identified as Fluopsin C, were tested against many pathogenic bacteria strains, proving their high antibiotic activity. In the present study, the bactericidal mechanisms of action of Fluopsin C and the semi-purified fraction F4A were elucidated. The results found in electron microscopy [scanning electron microscopy (SEM) and transmission electronic microscopy (TEM)] demonstrated that both Fluopsin C and F4A are affecting the cytoplasmatic membrane of Gram-positive and Gram-negative bacteria. These results were confirmed by fluorescence microscopy, where these bacteria presented permeabilization of their cytoplasmatic membranes after contact with the semi-purified fraction and pure compound. Using electronic and fluorescence microscopy, along with bacterial mutant strains with marked divisional septum, the membrane was defined as the primary target of Fluopsin C in the tested bacteria. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:03:24Z 2020-12-10T20:03:24Z 2020-06-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2020.01076 Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 11, 11 p., 2020. 1664-302X http://hdl.handle.net/11449/197012 10.3389/fmicb.2020.01076 WOS:000543335100001 |
url |
http://dx.doi.org/10.3389/fmicb.2020.01076 http://hdl.handle.net/11449/197012 |
identifier_str_mv |
Frontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 11, 11 p., 2020. 1664-302X 10.3389/fmicb.2020.01076 WOS:000543335100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129359123316736 |