Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cows

Detalhes bibliográficos
Autor(a) principal: Oliveira Júnior, Gerson A.
Data de Publicação: 2021
Outros Autores: Pinheiro, Vinícius G. [UNESP], Fonseca, Pablo A.S., Costa, Camila B. [UNESP], Pioltine, Elisa M. [UNESP], Botigelli, Ramon C. [UNESP], Razza, Eduardo M. [UNESP], Ereno, Ronaldo L. [UNESP], Ferraz, José B.S., Seneda, Marcelo M., Nogueira, Marcelo F.G. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.livsci.2021.104534
http://hdl.handle.net/11449/207751
Resumo: Antral follicle count (AFC) was phenotypically and genetically evaluated in a Bos taurus cow population. A total of 257 Aberdeen Angus cows underwent an ovarian ultrasound evaluation and hormonal synchronization for timed artificial insemination (TAI). These animals were between 40 and 70 d postpartum with a body score condition (BSC) between 2 and 4 (average = 2.7) on a scale of 1–5. The number of antral follicles was assessed 10 d before (D-10) the beginning of TAI. Visible follicles (≥3 mm in diameter) were counted from both ovaries (right and left) to determine the number of total follicles, and animals were allocated into groups according to the number of antral follicles: low AFC was defined as ≤ 15 follicles (n = 93); intermediate AFC as < 24 follicles and >16 follicles (n = 97); and high AFC as ≥ 25 follicles (n = 67). The comparison between the average AFC and pregnancy rate was performed using the chi-square test in the Minitab® 19.2 statistical software, adopting a P-value ≤ 0.05. Hair samples of Aberdeen Angus cows were collected for genomic DNA extraction and subsequent genotyping analysis using the GGP HD array. The associations between SNP markers and phenotypic information (AFC) were determined using the GBLUP methodology. Windows explaining more than 1% of the total additive genetic variance were selected as candidates for functional prospection using QTL colocalization and enrichment analysis using a hypergeometric test. The GWAS identified seven candidate genomic windows and 54 annotated genes within them. Among these genes, DUSP1, SOX17, XKR4, PLAG1, HFM1, PPM1K, IBSP, NCAPG-LCORL, and HMGHZ were associated with biological processes that might be related to follicle count in Angus cows. Enrichment analysis of the reproduction-related traits found enriched QTLs in BTA5 (“Inhibin level”, “Insulin-like growth factor 1 level”), BTA6 (“Calving ease”, “Calf size”, “Calving index”), and BTA14 (“Insulin-like growth factor 1 level”; “Age at puberty”, “Interval to first estrus after calving”). This study brings new knowledge of the genomic background of AFC and may lead to further investigations regarding physiological explanations for different AFCs and their relationship to fertility.
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spelling Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cowsBos taurusFertilityGenomicsReproductionAntral follicle count (AFC) was phenotypically and genetically evaluated in a Bos taurus cow population. A total of 257 Aberdeen Angus cows underwent an ovarian ultrasound evaluation and hormonal synchronization for timed artificial insemination (TAI). These animals were between 40 and 70 d postpartum with a body score condition (BSC) between 2 and 4 (average = 2.7) on a scale of 1–5. The number of antral follicles was assessed 10 d before (D-10) the beginning of TAI. Visible follicles (≥3 mm in diameter) were counted from both ovaries (right and left) to determine the number of total follicles, and animals were allocated into groups according to the number of antral follicles: low AFC was defined as ≤ 15 follicles (n = 93); intermediate AFC as < 24 follicles and >16 follicles (n = 97); and high AFC as ≥ 25 follicles (n = 67). The comparison between the average AFC and pregnancy rate was performed using the chi-square test in the Minitab® 19.2 statistical software, adopting a P-value ≤ 0.05. Hair samples of Aberdeen Angus cows were collected for genomic DNA extraction and subsequent genotyping analysis using the GGP HD array. The associations between SNP markers and phenotypic information (AFC) were determined using the GBLUP methodology. Windows explaining more than 1% of the total additive genetic variance were selected as candidates for functional prospection using QTL colocalization and enrichment analysis using a hypergeometric test. The GWAS identified seven candidate genomic windows and 54 annotated genes within them. Among these genes, DUSP1, SOX17, XKR4, PLAG1, HFM1, PPM1K, IBSP, NCAPG-LCORL, and HMGHZ were associated with biological processes that might be related to follicle count in Angus cows. Enrichment analysis of the reproduction-related traits found enriched QTLs in BTA5 (“Inhibin level”, “Insulin-like growth factor 1 level”), BTA6 (“Calving ease”, “Calf size”, “Calving index”), and BTA14 (“Insulin-like growth factor 1 level”; “Age at puberty”, “Interval to first estrus after calving”). This study brings new knowledge of the genomic background of AFC and may lead to further investigations regarding physiological explanations for different AFCs and their relationship to fertility.Department of Veterinary Medicine Faculty of Animal Science and Food Engineering University of São Paulo (USP)Center for Genetic Improvement of Livestock Department of Animal Biosciences University of GuelphLaboratory FitoFarmaTec Department of Pharmacology IBB São Paulo State University (UNESP)Department of Veterinary Clinics Londrina State University (UEL)Department of Biological Sciences School of Sciences and Languages UNESPLaboratory FitoFarmaTec Department of Pharmacology IBB São Paulo State University (UNESP)Department of Biological Sciences School of Sciences and Languages UNESPUniversidade de São Paulo (USP)University of GuelphUniversidade Estadual Paulista (Unesp)Universidade Estadual de Londrina (UEL)Oliveira Júnior, Gerson A.Pinheiro, Vinícius G. [UNESP]Fonseca, Pablo A.S.Costa, Camila B. [UNESP]Pioltine, Elisa M. [UNESP]Botigelli, Ramon C. [UNESP]Razza, Eduardo M. [UNESP]Ereno, Ronaldo L. [UNESP]Ferraz, José B.S.Seneda, Marcelo M.Nogueira, Marcelo F.G. [UNESP]2021-06-25T11:00:23Z2021-06-25T11:00:23Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.livsci.2021.104534Livestock Science, v. 249.1871-1413http://hdl.handle.net/11449/20775110.1016/j.livsci.2021.1045342-s2.0-85106214281Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Scienceinfo:eu-repo/semantics/openAccess2021-10-23T17:45:57Zoai:repositorio.unesp.br:11449/207751Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:45:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cows
title Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cows
spellingShingle Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cows
Oliveira Júnior, Gerson A.
Bos taurus
Fertility
Genomics
Reproduction
title_short Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cows
title_full Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cows
title_fullStr Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cows
title_full_unstemmed Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cows
title_sort Genomic and phenotypic analyses of antral follicle count in Aberdeen Angus cows
author Oliveira Júnior, Gerson A.
author_facet Oliveira Júnior, Gerson A.
Pinheiro, Vinícius G. [UNESP]
Fonseca, Pablo A.S.
Costa, Camila B. [UNESP]
Pioltine, Elisa M. [UNESP]
Botigelli, Ramon C. [UNESP]
Razza, Eduardo M. [UNESP]
Ereno, Ronaldo L. [UNESP]
Ferraz, José B.S.
Seneda, Marcelo M.
Nogueira, Marcelo F.G. [UNESP]
author_role author
author2 Pinheiro, Vinícius G. [UNESP]
Fonseca, Pablo A.S.
Costa, Camila B. [UNESP]
Pioltine, Elisa M. [UNESP]
Botigelli, Ramon C. [UNESP]
Razza, Eduardo M. [UNESP]
Ereno, Ronaldo L. [UNESP]
Ferraz, José B.S.
Seneda, Marcelo M.
Nogueira, Marcelo F.G. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
University of Guelph
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Londrina (UEL)
dc.contributor.author.fl_str_mv Oliveira Júnior, Gerson A.
Pinheiro, Vinícius G. [UNESP]
Fonseca, Pablo A.S.
Costa, Camila B. [UNESP]
Pioltine, Elisa M. [UNESP]
Botigelli, Ramon C. [UNESP]
Razza, Eduardo M. [UNESP]
Ereno, Ronaldo L. [UNESP]
Ferraz, José B.S.
Seneda, Marcelo M.
Nogueira, Marcelo F.G. [UNESP]
dc.subject.por.fl_str_mv Bos taurus
Fertility
Genomics
Reproduction
topic Bos taurus
Fertility
Genomics
Reproduction
description Antral follicle count (AFC) was phenotypically and genetically evaluated in a Bos taurus cow population. A total of 257 Aberdeen Angus cows underwent an ovarian ultrasound evaluation and hormonal synchronization for timed artificial insemination (TAI). These animals were between 40 and 70 d postpartum with a body score condition (BSC) between 2 and 4 (average = 2.7) on a scale of 1–5. The number of antral follicles was assessed 10 d before (D-10) the beginning of TAI. Visible follicles (≥3 mm in diameter) were counted from both ovaries (right and left) to determine the number of total follicles, and animals were allocated into groups according to the number of antral follicles: low AFC was defined as ≤ 15 follicles (n = 93); intermediate AFC as < 24 follicles and >16 follicles (n = 97); and high AFC as ≥ 25 follicles (n = 67). The comparison between the average AFC and pregnancy rate was performed using the chi-square test in the Minitab® 19.2 statistical software, adopting a P-value ≤ 0.05. Hair samples of Aberdeen Angus cows were collected for genomic DNA extraction and subsequent genotyping analysis using the GGP HD array. The associations between SNP markers and phenotypic information (AFC) were determined using the GBLUP methodology. Windows explaining more than 1% of the total additive genetic variance were selected as candidates for functional prospection using QTL colocalization and enrichment analysis using a hypergeometric test. The GWAS identified seven candidate genomic windows and 54 annotated genes within them. Among these genes, DUSP1, SOX17, XKR4, PLAG1, HFM1, PPM1K, IBSP, NCAPG-LCORL, and HMGHZ were associated with biological processes that might be related to follicle count in Angus cows. Enrichment analysis of the reproduction-related traits found enriched QTLs in BTA5 (“Inhibin level”, “Insulin-like growth factor 1 level”), BTA6 (“Calving ease”, “Calf size”, “Calving index”), and BTA14 (“Insulin-like growth factor 1 level”; “Age at puberty”, “Interval to first estrus after calving”). This study brings new knowledge of the genomic background of AFC and may lead to further investigations regarding physiological explanations for different AFCs and their relationship to fertility.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:00:23Z
2021-06-25T11:00:23Z
2021-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.livsci.2021.104534
Livestock Science, v. 249.
1871-1413
http://hdl.handle.net/11449/207751
10.1016/j.livsci.2021.104534
2-s2.0-85106214281
url http://dx.doi.org/10.1016/j.livsci.2021.104534
http://hdl.handle.net/11449/207751
identifier_str_mv Livestock Science, v. 249.
1871-1413
10.1016/j.livsci.2021.104534
2-s2.0-85106214281
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Livestock Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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