Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.snb.2019.126681 http://hdl.handle.net/11449/189245 |
Resumo: | The Zika virus has recently emerged as a major worldwide threat because it induces microencephaly and other brain damage in babies born to infected mothers. Epidemiological monitoring of infection has been hampered by the lack of reliable tests capable of distinguishing between Zika and other Flavivirus infections, especially the dengue virus. The present work describes the electrochemical modification of pencil carbon graphite electrodes with a material derived from 3-amino-4-hydroxybenzoic acid. Solutions were prepared in acid medium and the material formed showed insulating behavior. Optical and electrical data suggested that carboxyl groups did not participate in the reaction mechanism. FT-IR spectra indicated that these groups were retained, enabling the proposal of a mechanism based on the data obtained. The material formed was used for immobilization of the ssDNAzv aminated oligonucleotide. Detection of the hybridization was based on the Δip resulting from the higher electrostatic repulsion of [Fe(CN)6]3−, caused by the increase of phosphates by addition of the complementary target sequence. The system showed a good linear correlation coefficient (r² = 0.997), with satisfactory selectivity and reproducibility, a low limit of detection (25.4 pM), and precision (RSD) of 3.1%, using real samples of human serum enriched with total extract of Zika virus RNA. Tests of the system using the total RNA extracted from dengue virus types 2 and 3 cultures showed little difference in the observed current, hence confirming the specificity of the system. These findings demonstrated the potential of the proposed biosensor. |
id |
UNSP_a9faa2cfde233221f4639b80fa24de86 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/189245 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode3-Amino-4-hydroxybenzoic acid polymerElectrochemical genosensorPencil graphiteZika virusThe Zika virus has recently emerged as a major worldwide threat because it induces microencephaly and other brain damage in babies born to infected mothers. Epidemiological monitoring of infection has been hampered by the lack of reliable tests capable of distinguishing between Zika and other Flavivirus infections, especially the dengue virus. The present work describes the electrochemical modification of pencil carbon graphite electrodes with a material derived from 3-amino-4-hydroxybenzoic acid. Solutions were prepared in acid medium and the material formed showed insulating behavior. Optical and electrical data suggested that carboxyl groups did not participate in the reaction mechanism. FT-IR spectra indicated that these groups were retained, enabling the proposal of a mechanism based on the data obtained. The material formed was used for immobilization of the ssDNAzv aminated oligonucleotide. Detection of the hybridization was based on the Δip resulting from the higher electrostatic repulsion of [Fe(CN)6]3−, caused by the increase of phosphates by addition of the complementary target sequence. The system showed a good linear correlation coefficient (r² = 0.997), with satisfactory selectivity and reproducibility, a low limit of detection (25.4 pM), and precision (RSD) of 3.1%, using real samples of human serum enriched with total extract of Zika virus RNA. Tests of the system using the total RNA extracted from dengue virus types 2 and 3 cultures showed little difference in the observed current, hence confirming the specificity of the system. These findings demonstrated the potential of the proposed biosensor.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP)Grupo de Eletroquímica Aplicada a Polímeros e Sensores Instituto de Química Universidade Federal de UberlândiaOswaldo Cruz-Fiocruz Foundation Aggeu Magalhães Institute Cidade Universitária, Av. Prof. Moraes Rego s/nº, P.O. Box 7.472Biomedical Engineering Laboratory Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade UniversitariaDepartment of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP)CNPq: 440605/2016-4Universidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)Cidade UniversitáriaUniversidade Federal de Pernambuco (UFPE)da Fonseca Alves, Rafael [UNESP]Franco, Diego LeoniCordeiro, Marli Tenóriode Oliveira, Ernesto MarquesFireman Dutra, Rosa AmaliaDel Pilar Taboada Sotomayor, Maria [UNESP]2019-10-06T16:34:34Z2019-10-06T16:34:34Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.snb.2019.126681Sensors and Actuators, B: Chemical, v. 296.0925-4005http://hdl.handle.net/11449/18924510.1016/j.snb.2019.1266812-s2.0-85067244178Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSensors and Actuators, B: Chemicalinfo:eu-repo/semantics/openAccess2021-10-23T04:16:31Zoai:repositorio.unesp.br:11449/189245Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:02:52.806059Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode |
title |
Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode |
spellingShingle |
Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode da Fonseca Alves, Rafael [UNESP] 3-Amino-4-hydroxybenzoic acid polymer Electrochemical genosensor Pencil graphite Zika virus |
title_short |
Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode |
title_full |
Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode |
title_fullStr |
Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode |
title_full_unstemmed |
Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode |
title_sort |
Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode |
author |
da Fonseca Alves, Rafael [UNESP] |
author_facet |
da Fonseca Alves, Rafael [UNESP] Franco, Diego Leoni Cordeiro, Marli Tenório de Oliveira, Ernesto Marques Fireman Dutra, Rosa Amalia Del Pilar Taboada Sotomayor, Maria [UNESP] |
author_role |
author |
author2 |
Franco, Diego Leoni Cordeiro, Marli Tenório de Oliveira, Ernesto Marques Fireman Dutra, Rosa Amalia Del Pilar Taboada Sotomayor, Maria [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Uberlândia (UFU) Cidade Universitária Universidade Federal de Pernambuco (UFPE) |
dc.contributor.author.fl_str_mv |
da Fonseca Alves, Rafael [UNESP] Franco, Diego Leoni Cordeiro, Marli Tenório de Oliveira, Ernesto Marques Fireman Dutra, Rosa Amalia Del Pilar Taboada Sotomayor, Maria [UNESP] |
dc.subject.por.fl_str_mv |
3-Amino-4-hydroxybenzoic acid polymer Electrochemical genosensor Pencil graphite Zika virus |
topic |
3-Amino-4-hydroxybenzoic acid polymer Electrochemical genosensor Pencil graphite Zika virus |
description |
The Zika virus has recently emerged as a major worldwide threat because it induces microencephaly and other brain damage in babies born to infected mothers. Epidemiological monitoring of infection has been hampered by the lack of reliable tests capable of distinguishing between Zika and other Flavivirus infections, especially the dengue virus. The present work describes the electrochemical modification of pencil carbon graphite electrodes with a material derived from 3-amino-4-hydroxybenzoic acid. Solutions were prepared in acid medium and the material formed showed insulating behavior. Optical and electrical data suggested that carboxyl groups did not participate in the reaction mechanism. FT-IR spectra indicated that these groups were retained, enabling the proposal of a mechanism based on the data obtained. The material formed was used for immobilization of the ssDNAzv aminated oligonucleotide. Detection of the hybridization was based on the Δip resulting from the higher electrostatic repulsion of [Fe(CN)6]3−, caused by the increase of phosphates by addition of the complementary target sequence. The system showed a good linear correlation coefficient (r² = 0.997), with satisfactory selectivity and reproducibility, a low limit of detection (25.4 pM), and precision (RSD) of 3.1%, using real samples of human serum enriched with total extract of Zika virus RNA. Tests of the system using the total RNA extracted from dengue virus types 2 and 3 cultures showed little difference in the observed current, hence confirming the specificity of the system. These findings demonstrated the potential of the proposed biosensor. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:34:34Z 2019-10-06T16:34:34Z 2019-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.snb.2019.126681 Sensors and Actuators, B: Chemical, v. 296. 0925-4005 http://hdl.handle.net/11449/189245 10.1016/j.snb.2019.126681 2-s2.0-85067244178 |
url |
http://dx.doi.org/10.1016/j.snb.2019.126681 http://hdl.handle.net/11449/189245 |
identifier_str_mv |
Sensors and Actuators, B: Chemical, v. 296. 0925-4005 10.1016/j.snb.2019.126681 2-s2.0-85067244178 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sensors and Actuators, B: Chemical |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128599394353152 |