Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode

Detalhes bibliográficos
Autor(a) principal: da Fonseca Alves, Rafael [UNESP]
Data de Publicação: 2019
Outros Autores: Franco, Diego Leoni, Cordeiro, Marli Tenório, de Oliveira, Ernesto Marques, Fireman Dutra, Rosa Amalia, Del Pilar Taboada Sotomayor, Maria [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.snb.2019.126681
http://hdl.handle.net/11449/189245
Resumo: The Zika virus has recently emerged as a major worldwide threat because it induces microencephaly and other brain damage in babies born to infected mothers. Epidemiological monitoring of infection has been hampered by the lack of reliable tests capable of distinguishing between Zika and other Flavivirus infections, especially the dengue virus. The present work describes the electrochemical modification of pencil carbon graphite electrodes with a material derived from 3-amino-4-hydroxybenzoic acid. Solutions were prepared in acid medium and the material formed showed insulating behavior. Optical and electrical data suggested that carboxyl groups did not participate in the reaction mechanism. FT-IR spectra indicated that these groups were retained, enabling the proposal of a mechanism based on the data obtained. The material formed was used for immobilization of the ssDNAzv aminated oligonucleotide. Detection of the hybridization was based on the Δip resulting from the higher electrostatic repulsion of [Fe(CN)6]3−, caused by the increase of phosphates by addition of the complementary target sequence. The system showed a good linear correlation coefficient (r² = 0.997), with satisfactory selectivity and reproducibility, a low limit of detection (25.4 pM), and precision (RSD) of 3.1%, using real samples of human serum enriched with total extract of Zika virus RNA. Tests of the system using the total RNA extracted from dengue virus types 2 and 3 cultures showed little difference in the observed current, hence confirming the specificity of the system. These findings demonstrated the potential of the proposed biosensor.
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spelling Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode3-Amino-4-hydroxybenzoic acid polymerElectrochemical genosensorPencil graphiteZika virusThe Zika virus has recently emerged as a major worldwide threat because it induces microencephaly and other brain damage in babies born to infected mothers. Epidemiological monitoring of infection has been hampered by the lack of reliable tests capable of distinguishing between Zika and other Flavivirus infections, especially the dengue virus. The present work describes the electrochemical modification of pencil carbon graphite electrodes with a material derived from 3-amino-4-hydroxybenzoic acid. Solutions were prepared in acid medium and the material formed showed insulating behavior. Optical and electrical data suggested that carboxyl groups did not participate in the reaction mechanism. FT-IR spectra indicated that these groups were retained, enabling the proposal of a mechanism based on the data obtained. The material formed was used for immobilization of the ssDNAzv aminated oligonucleotide. Detection of the hybridization was based on the Δip resulting from the higher electrostatic repulsion of [Fe(CN)6]3−, caused by the increase of phosphates by addition of the complementary target sequence. The system showed a good linear correlation coefficient (r² = 0.997), with satisfactory selectivity and reproducibility, a low limit of detection (25.4 pM), and precision (RSD) of 3.1%, using real samples of human serum enriched with total extract of Zika virus RNA. Tests of the system using the total RNA extracted from dengue virus types 2 and 3 cultures showed little difference in the observed current, hence confirming the specificity of the system. These findings demonstrated the potential of the proposed biosensor.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP)Grupo de Eletroquímica Aplicada a Polímeros e Sensores Instituto de Química Universidade Federal de UberlândiaOswaldo Cruz-Fiocruz Foundation Aggeu Magalhães Institute Cidade Universitária, Av. Prof. Moraes Rego s/nº, P.O. Box 7.472Biomedical Engineering Laboratory Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade UniversitariaDepartment of Analytical Chemistry Institute of Chemistry State University of São Paulo (UNESP)CNPq: 440605/2016-4Universidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)Cidade UniversitáriaUniversidade Federal de Pernambuco (UFPE)da Fonseca Alves, Rafael [UNESP]Franco, Diego LeoniCordeiro, Marli Tenóriode Oliveira, Ernesto MarquesFireman Dutra, Rosa AmaliaDel Pilar Taboada Sotomayor, Maria [UNESP]2019-10-06T16:34:34Z2019-10-06T16:34:34Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.snb.2019.126681Sensors and Actuators, B: Chemical, v. 296.0925-4005http://hdl.handle.net/11449/18924510.1016/j.snb.2019.1266812-s2.0-85067244178Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSensors and Actuators, B: Chemicalinfo:eu-repo/semantics/openAccess2021-10-23T04:16:31Zoai:repositorio.unesp.br:11449/189245Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:02:52.806059Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode
title Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode
spellingShingle Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode
da Fonseca Alves, Rafael [UNESP]
3-Amino-4-hydroxybenzoic acid polymer
Electrochemical genosensor
Pencil graphite
Zika virus
title_short Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode
title_full Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode
title_fullStr Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode
title_full_unstemmed Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode
title_sort Novel electrochemical genosensor for Zika virus based on a poly-(3-amino-4-hydroxybenzoic acid)-modified pencil carbon graphite electrode
author da Fonseca Alves, Rafael [UNESP]
author_facet da Fonseca Alves, Rafael [UNESP]
Franco, Diego Leoni
Cordeiro, Marli Tenório
de Oliveira, Ernesto Marques
Fireman Dutra, Rosa Amalia
Del Pilar Taboada Sotomayor, Maria [UNESP]
author_role author
author2 Franco, Diego Leoni
Cordeiro, Marli Tenório
de Oliveira, Ernesto Marques
Fireman Dutra, Rosa Amalia
Del Pilar Taboada Sotomayor, Maria [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Uberlândia (UFU)
Cidade Universitária
Universidade Federal de Pernambuco (UFPE)
dc.contributor.author.fl_str_mv da Fonseca Alves, Rafael [UNESP]
Franco, Diego Leoni
Cordeiro, Marli Tenório
de Oliveira, Ernesto Marques
Fireman Dutra, Rosa Amalia
Del Pilar Taboada Sotomayor, Maria [UNESP]
dc.subject.por.fl_str_mv 3-Amino-4-hydroxybenzoic acid polymer
Electrochemical genosensor
Pencil graphite
Zika virus
topic 3-Amino-4-hydroxybenzoic acid polymer
Electrochemical genosensor
Pencil graphite
Zika virus
description The Zika virus has recently emerged as a major worldwide threat because it induces microencephaly and other brain damage in babies born to infected mothers. Epidemiological monitoring of infection has been hampered by the lack of reliable tests capable of distinguishing between Zika and other Flavivirus infections, especially the dengue virus. The present work describes the electrochemical modification of pencil carbon graphite electrodes with a material derived from 3-amino-4-hydroxybenzoic acid. Solutions were prepared in acid medium and the material formed showed insulating behavior. Optical and electrical data suggested that carboxyl groups did not participate in the reaction mechanism. FT-IR spectra indicated that these groups were retained, enabling the proposal of a mechanism based on the data obtained. The material formed was used for immobilization of the ssDNAzv aminated oligonucleotide. Detection of the hybridization was based on the Δip resulting from the higher electrostatic repulsion of [Fe(CN)6]3−, caused by the increase of phosphates by addition of the complementary target sequence. The system showed a good linear correlation coefficient (r² = 0.997), with satisfactory selectivity and reproducibility, a low limit of detection (25.4 pM), and precision (RSD) of 3.1%, using real samples of human serum enriched with total extract of Zika virus RNA. Tests of the system using the total RNA extracted from dengue virus types 2 and 3 cultures showed little difference in the observed current, hence confirming the specificity of the system. These findings demonstrated the potential of the proposed biosensor.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:34:34Z
2019-10-06T16:34:34Z
2019-10-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.snb.2019.126681
Sensors and Actuators, B: Chemical, v. 296.
0925-4005
http://hdl.handle.net/11449/189245
10.1016/j.snb.2019.126681
2-s2.0-85067244178
url http://dx.doi.org/10.1016/j.snb.2019.126681
http://hdl.handle.net/11449/189245
identifier_str_mv Sensors and Actuators, B: Chemical, v. 296.
0925-4005
10.1016/j.snb.2019.126681
2-s2.0-85067244178
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sensors and Actuators, B: Chemical
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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