In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/sj.ejcn.1602158 http://hdl.handle.net/11449/230989 |
Resumo: | Objective: This in vitro study compared the adhesion of two probiotics microorganisms (Lactobacillus casei Shirota and Lactobacillus acidophilus) to an artificial caries model. Method: In total, 30 bovine teeth were longitudinally sectioned, excluding the lingual half surface. The specimens were covered with nail varnish, except for an area of 3 by 5 mm in dentin, suspended in an artificial caries solution promoted by Streptococcus mutans, and incubated at 37°C. After 14 days, the specimens were separated equally into two groups and transferred to a brain heart infusion culture media containing L. acidophilus (group A) and L. casei Shirota (group S), at 37°C. After 48 h, the exposed area of dentin was washed with 1 ml of distilled water and the caries dentin was removed and dispersed in 1 ml of saline solution. The samples of distilled water and caries dentin were diluted and inoculated in Rogosa selective Lactobacillus agar. The results in CFU/ml were analysed by the Mann-Whitney test. Results: There was no significant difference between Groups A and S for the lactobacilli count in samples of distilled water (P=0.237). Conclusions: The amount of L. acidophilus in the artificially caries dentin was significantly superior compared to L. casei Shirota (P=0.047), suggesting an inferior adhesion potential for this microorganism. © 2005 Nature Publishing Group. All rights reserved. |
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In vitro evaluation of probiotics microorganisms adhesion to an artificial caries modelCultured milk productsDental cariesLactobacillus acidophilusLactobacillus caseiObjective: This in vitro study compared the adhesion of two probiotics microorganisms (Lactobacillus casei Shirota and Lactobacillus acidophilus) to an artificial caries model. Method: In total, 30 bovine teeth were longitudinally sectioned, excluding the lingual half surface. The specimens were covered with nail varnish, except for an area of 3 by 5 mm in dentin, suspended in an artificial caries solution promoted by Streptococcus mutans, and incubated at 37°C. After 14 days, the specimens were separated equally into two groups and transferred to a brain heart infusion culture media containing L. acidophilus (group A) and L. casei Shirota (group S), at 37°C. After 48 h, the exposed area of dentin was washed with 1 ml of distilled water and the caries dentin was removed and dispersed in 1 ml of saline solution. The samples of distilled water and caries dentin were diluted and inoculated in Rogosa selective Lactobacillus agar. The results in CFU/ml were analysed by the Mann-Whitney test. Results: There was no significant difference between Groups A and S for the lactobacilli count in samples of distilled water (P=0.237). Conclusions: The amount of L. acidophilus in the artificially caries dentin was significantly superior compared to L. casei Shirota (P=0.047), suggesting an inferior adhesion potential for this microorganism. © 2005 Nature Publishing Group. All rights reserved.Department of Pediatric Dentistry Araraquara Dental School University of São Paulo State, Rua Humaitá 1680, 305 AraraquaraUniversidade de São Paulo (USP)Lima, Lu M.Motisuki, C.Palomari Spolidorio, D. MadalenaSantos Pinto, L.2022-04-29T08:42:58Z2022-04-29T08:42:58Z2005-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article884-886http://dx.doi.org/10.1038/sj.ejcn.1602158European Journal of Clinical Nutrition, v. 59, n. 7, p. 884-886, 2005.0954-3007http://hdl.handle.net/11449/23098910.1038/sj.ejcn.16021582-s2.0-22644441840Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Clinical Nutritioninfo:eu-repo/semantics/openAccess2024-09-26T14:21:35Zoai:repositorio.unesp.br:11449/230989Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T14:21:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model |
title |
In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model |
spellingShingle |
In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model Lima, Lu M. Cultured milk products Dental caries Lactobacillus acidophilus Lactobacillus casei |
title_short |
In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model |
title_full |
In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model |
title_fullStr |
In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model |
title_full_unstemmed |
In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model |
title_sort |
In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model |
author |
Lima, Lu M. |
author_facet |
Lima, Lu M. Motisuki, C. Palomari Spolidorio, D. Madalena Santos Pinto, L. |
author_role |
author |
author2 |
Motisuki, C. Palomari Spolidorio, D. Madalena Santos Pinto, L. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Lima, Lu M. Motisuki, C. Palomari Spolidorio, D. Madalena Santos Pinto, L. |
dc.subject.por.fl_str_mv |
Cultured milk products Dental caries Lactobacillus acidophilus Lactobacillus casei |
topic |
Cultured milk products Dental caries Lactobacillus acidophilus Lactobacillus casei |
description |
Objective: This in vitro study compared the adhesion of two probiotics microorganisms (Lactobacillus casei Shirota and Lactobacillus acidophilus) to an artificial caries model. Method: In total, 30 bovine teeth were longitudinally sectioned, excluding the lingual half surface. The specimens were covered with nail varnish, except for an area of 3 by 5 mm in dentin, suspended in an artificial caries solution promoted by Streptococcus mutans, and incubated at 37°C. After 14 days, the specimens were separated equally into two groups and transferred to a brain heart infusion culture media containing L. acidophilus (group A) and L. casei Shirota (group S), at 37°C. After 48 h, the exposed area of dentin was washed with 1 ml of distilled water and the caries dentin was removed and dispersed in 1 ml of saline solution. The samples of distilled water and caries dentin were diluted and inoculated in Rogosa selective Lactobacillus agar. The results in CFU/ml were analysed by the Mann-Whitney test. Results: There was no significant difference between Groups A and S for the lactobacilli count in samples of distilled water (P=0.237). Conclusions: The amount of L. acidophilus in the artificially caries dentin was significantly superior compared to L. casei Shirota (P=0.047), suggesting an inferior adhesion potential for this microorganism. © 2005 Nature Publishing Group. All rights reserved. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-07-01 2022-04-29T08:42:58Z 2022-04-29T08:42:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/sj.ejcn.1602158 European Journal of Clinical Nutrition, v. 59, n. 7, p. 884-886, 2005. 0954-3007 http://hdl.handle.net/11449/230989 10.1038/sj.ejcn.1602158 2-s2.0-22644441840 |
url |
http://dx.doi.org/10.1038/sj.ejcn.1602158 http://hdl.handle.net/11449/230989 |
identifier_str_mv |
European Journal of Clinical Nutrition, v. 59, n. 7, p. 884-886, 2005. 0954-3007 10.1038/sj.ejcn.1602158 2-s2.0-22644441840 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
European Journal of Clinical Nutrition |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
884-886 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546435766910976 |