In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model

Detalhes bibliográficos
Autor(a) principal: Lima, Lu M.
Data de Publicação: 2005
Outros Autores: Motisuki, C., Palomari Spolidorio, D. Madalena, Santos Pinto, L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1038/sj.ejcn.1602158
http://hdl.handle.net/11449/230989
Resumo: Objective: This in vitro study compared the adhesion of two probiotics microorganisms (Lactobacillus casei Shirota and Lactobacillus acidophilus) to an artificial caries model. Method: In total, 30 bovine teeth were longitudinally sectioned, excluding the lingual half surface. The specimens were covered with nail varnish, except for an area of 3 by 5 mm in dentin, suspended in an artificial caries solution promoted by Streptococcus mutans, and incubated at 37°C. After 14 days, the specimens were separated equally into two groups and transferred to a brain heart infusion culture media containing L. acidophilus (group A) and L. casei Shirota (group S), at 37°C. After 48 h, the exposed area of dentin was washed with 1 ml of distilled water and the caries dentin was removed and dispersed in 1 ml of saline solution. The samples of distilled water and caries dentin were diluted and inoculated in Rogosa selective Lactobacillus agar. The results in CFU/ml were analysed by the Mann-Whitney test. Results: There was no significant difference between Groups A and S for the lactobacilli count in samples of distilled water (P=0.237). Conclusions: The amount of L. acidophilus in the artificially caries dentin was significantly superior compared to L. casei Shirota (P=0.047), suggesting an inferior adhesion potential for this microorganism. © 2005 Nature Publishing Group. All rights reserved.
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spelling In vitro evaluation of probiotics microorganisms adhesion to an artificial caries modelCultured milk productsDental cariesLactobacillus acidophilusLactobacillus caseiObjective: This in vitro study compared the adhesion of two probiotics microorganisms (Lactobacillus casei Shirota and Lactobacillus acidophilus) to an artificial caries model. Method: In total, 30 bovine teeth were longitudinally sectioned, excluding the lingual half surface. The specimens were covered with nail varnish, except for an area of 3 by 5 mm in dentin, suspended in an artificial caries solution promoted by Streptococcus mutans, and incubated at 37°C. After 14 days, the specimens were separated equally into two groups and transferred to a brain heart infusion culture media containing L. acidophilus (group A) and L. casei Shirota (group S), at 37°C. After 48 h, the exposed area of dentin was washed with 1 ml of distilled water and the caries dentin was removed and dispersed in 1 ml of saline solution. The samples of distilled water and caries dentin were diluted and inoculated in Rogosa selective Lactobacillus agar. The results in CFU/ml were analysed by the Mann-Whitney test. Results: There was no significant difference between Groups A and S for the lactobacilli count in samples of distilled water (P=0.237). Conclusions: The amount of L. acidophilus in the artificially caries dentin was significantly superior compared to L. casei Shirota (P=0.047), suggesting an inferior adhesion potential for this microorganism. © 2005 Nature Publishing Group. All rights reserved.Department of Pediatric Dentistry Araraquara Dental School University of São Paulo State, Rua Humaitá 1680, 305 AraraquaraUniversidade de São Paulo (USP)Lima, Lu M.Motisuki, C.Palomari Spolidorio, D. MadalenaSantos Pinto, L.2022-04-29T08:42:58Z2022-04-29T08:42:58Z2005-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article884-886http://dx.doi.org/10.1038/sj.ejcn.1602158European Journal of Clinical Nutrition, v. 59, n. 7, p. 884-886, 2005.0954-3007http://hdl.handle.net/11449/23098910.1038/sj.ejcn.16021582-s2.0-22644441840Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Clinical Nutritioninfo:eu-repo/semantics/openAccess2024-09-26T14:21:35Zoai:repositorio.unesp.br:11449/230989Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T14:21:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model
title In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model
spellingShingle In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model
Lima, Lu M.
Cultured milk products
Dental caries
Lactobacillus acidophilus
Lactobacillus casei
title_short In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model
title_full In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model
title_fullStr In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model
title_full_unstemmed In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model
title_sort In vitro evaluation of probiotics microorganisms adhesion to an artificial caries model
author Lima, Lu M.
author_facet Lima, Lu M.
Motisuki, C.
Palomari Spolidorio, D. Madalena
Santos Pinto, L.
author_role author
author2 Motisuki, C.
Palomari Spolidorio, D. Madalena
Santos Pinto, L.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Lima, Lu M.
Motisuki, C.
Palomari Spolidorio, D. Madalena
Santos Pinto, L.
dc.subject.por.fl_str_mv Cultured milk products
Dental caries
Lactobacillus acidophilus
Lactobacillus casei
topic Cultured milk products
Dental caries
Lactobacillus acidophilus
Lactobacillus casei
description Objective: This in vitro study compared the adhesion of two probiotics microorganisms (Lactobacillus casei Shirota and Lactobacillus acidophilus) to an artificial caries model. Method: In total, 30 bovine teeth were longitudinally sectioned, excluding the lingual half surface. The specimens were covered with nail varnish, except for an area of 3 by 5 mm in dentin, suspended in an artificial caries solution promoted by Streptococcus mutans, and incubated at 37°C. After 14 days, the specimens were separated equally into two groups and transferred to a brain heart infusion culture media containing L. acidophilus (group A) and L. casei Shirota (group S), at 37°C. After 48 h, the exposed area of dentin was washed with 1 ml of distilled water and the caries dentin was removed and dispersed in 1 ml of saline solution. The samples of distilled water and caries dentin were diluted and inoculated in Rogosa selective Lactobacillus agar. The results in CFU/ml were analysed by the Mann-Whitney test. Results: There was no significant difference between Groups A and S for the lactobacilli count in samples of distilled water (P=0.237). Conclusions: The amount of L. acidophilus in the artificially caries dentin was significantly superior compared to L. casei Shirota (P=0.047), suggesting an inferior adhesion potential for this microorganism. © 2005 Nature Publishing Group. All rights reserved.
publishDate 2005
dc.date.none.fl_str_mv 2005-07-01
2022-04-29T08:42:58Z
2022-04-29T08:42:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1038/sj.ejcn.1602158
European Journal of Clinical Nutrition, v. 59, n. 7, p. 884-886, 2005.
0954-3007
http://hdl.handle.net/11449/230989
10.1038/sj.ejcn.1602158
2-s2.0-22644441840
url http://dx.doi.org/10.1038/sj.ejcn.1602158
http://hdl.handle.net/11449/230989
identifier_str_mv European Journal of Clinical Nutrition, v. 59, n. 7, p. 884-886, 2005.
0954-3007
10.1038/sj.ejcn.1602158
2-s2.0-22644441840
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv European Journal of Clinical Nutrition
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 884-886
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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