Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows

Detalhes bibliográficos
Autor(a) principal: Juliano, L. C.B. [UNESP]
Data de Publicação: 2022
Outros Autores: Gouvêa, F. L.R. [UNESP], Latosinski, G. S. [UNESP], Fabri, F. H.H. [UNESP], Salvador, T. B. [UNESP], Guimaraes, F. F. [UNESP], Ribeiro, M. G. [UNESP], Langoni, H. [UNESP], Rall, V. L.M. [UNESP], Hernandes, R. T. [UNESP], Leite, D. S., Pantoja, J. C.F. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/lam.13768
http://hdl.handle.net/11449/240341
Resumo: The objectives were to study the diversity of Enterococcus spp. isolated from mastitis cases, milking equipment and the environment of dairy cows; and to determine in vitro resistance of isolates to antimicrobials that are relevant to human and animal health. Ten dairy farms were visited to collect samples from mastitis cases, faeces, bedding, aisles, water and milking equipment. Identification of Enterococcus at the species level and antimicrobial resistance testing was performed by MALDI-TOF and the disk-diffusion method, respectively. Of 365 isolates, Enterococcus hirae was the most prevalent, being more likely to be isolated from faeces than from milk (odds ratio (OR) = 39·2), liners (OR = 5·4) or bedding (OR = 2·2). Enterococcus saccharolyticus was the most prevalent in milk samples. The chances of isolating Enterococcus faecalis from milk were higher than from aisles (OR = 12·5), faeces (OR = 5·3), bedding (OR = 3·6) or liners (OR = 3·0). The odds of isolating Enterococcus faecium from faeces were higher than from liners (OR = 7·3), bedding (OR = 2·5) or aisles (OR = 2·4). Of 360 tested isolates, 1·9, 0·3 and 0·6% were resistant to penicillin, vancomycin and teicoplanin, respectively. Our results suggest that Enterococcus species can occupy specific ecological niches on dairy farms and pose a risk to public and animal health.
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spelling Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cowsantimicrobialsdairydiversityenvironmentalresistanceThe objectives were to study the diversity of Enterococcus spp. isolated from mastitis cases, milking equipment and the environment of dairy cows; and to determine in vitro resistance of isolates to antimicrobials that are relevant to human and animal health. Ten dairy farms were visited to collect samples from mastitis cases, faeces, bedding, aisles, water and milking equipment. Identification of Enterococcus at the species level and antimicrobial resistance testing was performed by MALDI-TOF and the disk-diffusion method, respectively. Of 365 isolates, Enterococcus hirae was the most prevalent, being more likely to be isolated from faeces than from milk (odds ratio (OR) = 39·2), liners (OR = 5·4) or bedding (OR = 2·2). Enterococcus saccharolyticus was the most prevalent in milk samples. The chances of isolating Enterococcus faecalis from milk were higher than from aisles (OR = 12·5), faeces (OR = 5·3), bedding (OR = 3·6) or liners (OR = 3·0). The odds of isolating Enterococcus faecium from faeces were higher than from liners (OR = 7·3), bedding (OR = 2·5) or aisles (OR = 2·4). Of 360 tested isolates, 1·9, 0·3 and 0·6% were resistant to penicillin, vancomycin and teicoplanin, respectively. Our results suggest that Enterococcus species can occupy specific ecological niches on dairy farms and pose a risk to public and animal health.Department of Animal Production and Preventive Veterinary Medicine Sao Paulo State University (UNESP), SPDepartment of Chemical and Biological Sciences Institute of Biosciences Sao Paulo State University (UNESP), SPDepartment of Genetics Evolution Microbiology and Immunology University of Campinas, SPDepartment of Animal Production and Preventive Veterinary Medicine Sao Paulo State University (UNESP), SPDepartment of Chemical and Biological Sciences Institute of Biosciences Sao Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Juliano, L. C.B. [UNESP]Gouvêa, F. L.R. [UNESP]Latosinski, G. S. [UNESP]Fabri, F. H.H. [UNESP]Salvador, T. B. [UNESP]Guimaraes, F. F. [UNESP]Ribeiro, M. G. [UNESP]Langoni, H. [UNESP]Rall, V. L.M. [UNESP]Hernandes, R. T. [UNESP]Leite, D. S.Pantoja, J. C.F. [UNESP]2023-03-01T20:12:46Z2023-03-01T20:12:46Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/lam.13768Letters in Applied Microbiology.1472-765X0266-8254http://hdl.handle.net/11449/24034110.1111/lam.137682-s2.0-85132783651Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLetters in Applied Microbiologyinfo:eu-repo/semantics/openAccess2023-03-01T20:12:46Zoai:repositorio.unesp.br:11449/240341Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:12:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows
title Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows
spellingShingle Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows
Juliano, L. C.B. [UNESP]
antimicrobials
dairy
diversity
environmental
resistance
title_short Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows
title_full Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows
title_fullStr Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows
title_full_unstemmed Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows
title_sort Species diversity and antimicrobial resistance patterns of Enterococcus spp. isolated from mastitis cases, milking machine and the environment of dairy cows
author Juliano, L. C.B. [UNESP]
author_facet Juliano, L. C.B. [UNESP]
Gouvêa, F. L.R. [UNESP]
Latosinski, G. S. [UNESP]
Fabri, F. H.H. [UNESP]
Salvador, T. B. [UNESP]
Guimaraes, F. F. [UNESP]
Ribeiro, M. G. [UNESP]
Langoni, H. [UNESP]
Rall, V. L.M. [UNESP]
Hernandes, R. T. [UNESP]
Leite, D. S.
Pantoja, J. C.F. [UNESP]
author_role author
author2 Gouvêa, F. L.R. [UNESP]
Latosinski, G. S. [UNESP]
Fabri, F. H.H. [UNESP]
Salvador, T. B. [UNESP]
Guimaraes, F. F. [UNESP]
Ribeiro, M. G. [UNESP]
Langoni, H. [UNESP]
Rall, V. L.M. [UNESP]
Hernandes, R. T. [UNESP]
Leite, D. S.
Pantoja, J. C.F. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Juliano, L. C.B. [UNESP]
Gouvêa, F. L.R. [UNESP]
Latosinski, G. S. [UNESP]
Fabri, F. H.H. [UNESP]
Salvador, T. B. [UNESP]
Guimaraes, F. F. [UNESP]
Ribeiro, M. G. [UNESP]
Langoni, H. [UNESP]
Rall, V. L.M. [UNESP]
Hernandes, R. T. [UNESP]
Leite, D. S.
Pantoja, J. C.F. [UNESP]
dc.subject.por.fl_str_mv antimicrobials
dairy
diversity
environmental
resistance
topic antimicrobials
dairy
diversity
environmental
resistance
description The objectives were to study the diversity of Enterococcus spp. isolated from mastitis cases, milking equipment and the environment of dairy cows; and to determine in vitro resistance of isolates to antimicrobials that are relevant to human and animal health. Ten dairy farms were visited to collect samples from mastitis cases, faeces, bedding, aisles, water and milking equipment. Identification of Enterococcus at the species level and antimicrobial resistance testing was performed by MALDI-TOF and the disk-diffusion method, respectively. Of 365 isolates, Enterococcus hirae was the most prevalent, being more likely to be isolated from faeces than from milk (odds ratio (OR) = 39·2), liners (OR = 5·4) or bedding (OR = 2·2). Enterococcus saccharolyticus was the most prevalent in milk samples. The chances of isolating Enterococcus faecalis from milk were higher than from aisles (OR = 12·5), faeces (OR = 5·3), bedding (OR = 3·6) or liners (OR = 3·0). The odds of isolating Enterococcus faecium from faeces were higher than from liners (OR = 7·3), bedding (OR = 2·5) or aisles (OR = 2·4). Of 360 tested isolates, 1·9, 0·3 and 0·6% were resistant to penicillin, vancomycin and teicoplanin, respectively. Our results suggest that Enterococcus species can occupy specific ecological niches on dairy farms and pose a risk to public and animal health.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-03-01T20:12:46Z
2023-03-01T20:12:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/lam.13768
Letters in Applied Microbiology.
1472-765X
0266-8254
http://hdl.handle.net/11449/240341
10.1111/lam.13768
2-s2.0-85132783651
url http://dx.doi.org/10.1111/lam.13768
http://hdl.handle.net/11449/240341
identifier_str_mv Letters in Applied Microbiology.
1472-765X
0266-8254
10.1111/lam.13768
2-s2.0-85132783651
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Letters in Applied Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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