Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitis

Detalhes bibliográficos
Autor(a) principal: Garcia de Carvalho, Gabriel [UNESP]
Data de Publicação: 2020
Outros Autores: Sanchez-Puetate, Julio Cesar [UNESP], Donatoni, Maria Carolina, Maquera Huacho, Patricia Milagros [UNESP], de Souza Rastelli, Alessandra Nara [UNESP], de Oliveira, Kleber Thiago, Palomari Spolidorio, Denise Madalena [UNESP], Leal Zandim-Barcelos, Daniela [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.pdpdt.2020.101916
http://hdl.handle.net/11449/199117
Resumo: Chlorin-e6 (Ce6), as a photosensitizer (PS), has demonstrated significant reduction of microorganisms’ viability when irradiated by red light. However, the main absorption peak of this PS is located at blue light spectrum, which is less investigated. This study aimed to evaluate the effect of pure-chlorin-e6-mediated photodynamic inactivation (PDI) using different light sources (450 or 660 nm) against biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans single-species biofilms were developed under proper conditions for five days. PDI was performed using different concentrations of Ce6 (100 and 200 mM), wavelengths (450 and 660 nm) and comparisons were made after colony forming unit and confocal laser scanning microscopy (CLSM) analysis. The use of light and PS were also individually tested. The greatest bacterial elimination was observed in the group where PDI was employed with blue light and concentration of 200 mM for all bacterial strains tested (4.01 log10 for A. actinomycetemcomitans, and total elimination for P. gingivalis and S. oralis), except for F. nucleatum, where 3.46 log10 reduction was observed when red light and 200 mM Ce6 were applied (p < 0.05). The antimicrobial effects of PDI mediated by Ce6 for all single pathogenic biofilms were confirmed by live/dead staining under CLSM analysis. For all single-species biofilms, the use of PDI mediated by chlorin-e6 photosensitizer under blue or red-light irradiation (450 and 660 nm) demonstrated a significant reduction in bacterial viability, but blue light showed a promising higher photobiological effect, encouraging its adjuvant use to basic periodontitis treatment.
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spelling Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitisAntimicrobialPeriodontitisPhotochemotherapyPhotodynamic TherapyPhotodynamic therapyPhotosensitizing agentsChlorin-e6 (Ce6), as a photosensitizer (PS), has demonstrated significant reduction of microorganisms’ viability when irradiated by red light. However, the main absorption peak of this PS is located at blue light spectrum, which is less investigated. This study aimed to evaluate the effect of pure-chlorin-e6-mediated photodynamic inactivation (PDI) using different light sources (450 or 660 nm) against biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans single-species biofilms were developed under proper conditions for five days. PDI was performed using different concentrations of Ce6 (100 and 200 mM), wavelengths (450 and 660 nm) and comparisons were made after colony forming unit and confocal laser scanning microscopy (CLSM) analysis. The use of light and PS were also individually tested. The greatest bacterial elimination was observed in the group where PDI was employed with blue light and concentration of 200 mM for all bacterial strains tested (4.01 log10 for A. actinomycetemcomitans, and total elimination for P. gingivalis and S. oralis), except for F. nucleatum, where 3.46 log10 reduction was observed when red light and 200 mM Ce6 were applied (p < 0.05). The antimicrobial effects of PDI mediated by Ce6 for all single pathogenic biofilms were confirmed by live/dead staining under CLSM analysis. For all single-species biofilms, the use of PDI mediated by chlorin-e6 photosensitizer under blue or red-light irradiation (450 and 660 nm) demonstrated a significant reduction in bacterial viability, but blue light showed a promising higher photobiological effect, encouraging its adjuvant use to basic periodontitis treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Diagnosis and Surgery São Paulo State University (Unesp) School of Dentistry, Rua Humaitá, 1680Department of Chemistry Federal University of São Carlos (UFSCar)Department of Physiology and Pathology São Paulo State University (Unesp) School of Dentistry, Rua HumaitáDepartment of Restorative Dentistry São Paulo State University (Unesp) School of Dentistry, Rua Humaitá, 1680Department of Diagnosis and Surgery São Paulo State University (Unesp) School of Dentistry, Rua Humaitá, 1680Department of Physiology and Pathology São Paulo State University (Unesp) School of Dentistry, Rua HumaitáDepartment of Restorative Dentistry São Paulo State University (Unesp) School of Dentistry, Rua Humaitá, 1680FAPESP: 2013/07276-1FAPESP: 2018/00106-7Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Garcia de Carvalho, Gabriel [UNESP]Sanchez-Puetate, Julio Cesar [UNESP]Donatoni, Maria CarolinaMaquera Huacho, Patricia Milagros [UNESP]de Souza Rastelli, Alessandra Nara [UNESP]de Oliveira, Kleber ThiagoPalomari Spolidorio, Denise Madalena [UNESP]Leal Zandim-Barcelos, Daniela [UNESP]2020-12-12T01:31:11Z2020-12-12T01:31:11Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2020.101916Photodiagnosis and Photodynamic Therapy, v. 31.1873-15971572-1000http://hdl.handle.net/11449/19911710.1016/j.pdpdt.2020.1019162-s2.0-85088113623Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2021-10-23T03:12:47Zoai:repositorio.unesp.br:11449/199117Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:49:39.950736Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitis
title Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitis
spellingShingle Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitis
Garcia de Carvalho, Gabriel [UNESP]
Antimicrobial
Periodontitis
Photochemotherapy
Photodynamic Therapy
Photodynamic therapy
Photosensitizing agents
title_short Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitis
title_full Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitis
title_fullStr Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitis
title_full_unstemmed Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitis
title_sort Photodynamic inactivation using a chlorin-based photosensitizer with blue or red-light irradiation against single-species biofilms related to periodontitis
author Garcia de Carvalho, Gabriel [UNESP]
author_facet Garcia de Carvalho, Gabriel [UNESP]
Sanchez-Puetate, Julio Cesar [UNESP]
Donatoni, Maria Carolina
Maquera Huacho, Patricia Milagros [UNESP]
de Souza Rastelli, Alessandra Nara [UNESP]
de Oliveira, Kleber Thiago
Palomari Spolidorio, Denise Madalena [UNESP]
Leal Zandim-Barcelos, Daniela [UNESP]
author_role author
author2 Sanchez-Puetate, Julio Cesar [UNESP]
Donatoni, Maria Carolina
Maquera Huacho, Patricia Milagros [UNESP]
de Souza Rastelli, Alessandra Nara [UNESP]
de Oliveira, Kleber Thiago
Palomari Spolidorio, Denise Madalena [UNESP]
Leal Zandim-Barcelos, Daniela [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv Garcia de Carvalho, Gabriel [UNESP]
Sanchez-Puetate, Julio Cesar [UNESP]
Donatoni, Maria Carolina
Maquera Huacho, Patricia Milagros [UNESP]
de Souza Rastelli, Alessandra Nara [UNESP]
de Oliveira, Kleber Thiago
Palomari Spolidorio, Denise Madalena [UNESP]
Leal Zandim-Barcelos, Daniela [UNESP]
dc.subject.por.fl_str_mv Antimicrobial
Periodontitis
Photochemotherapy
Photodynamic Therapy
Photodynamic therapy
Photosensitizing agents
topic Antimicrobial
Periodontitis
Photochemotherapy
Photodynamic Therapy
Photodynamic therapy
Photosensitizing agents
description Chlorin-e6 (Ce6), as a photosensitizer (PS), has demonstrated significant reduction of microorganisms’ viability when irradiated by red light. However, the main absorption peak of this PS is located at blue light spectrum, which is less investigated. This study aimed to evaluate the effect of pure-chlorin-e6-mediated photodynamic inactivation (PDI) using different light sources (450 or 660 nm) against biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans single-species biofilms were developed under proper conditions for five days. PDI was performed using different concentrations of Ce6 (100 and 200 mM), wavelengths (450 and 660 nm) and comparisons were made after colony forming unit and confocal laser scanning microscopy (CLSM) analysis. The use of light and PS were also individually tested. The greatest bacterial elimination was observed in the group where PDI was employed with blue light and concentration of 200 mM for all bacterial strains tested (4.01 log10 for A. actinomycetemcomitans, and total elimination for P. gingivalis and S. oralis), except for F. nucleatum, where 3.46 log10 reduction was observed when red light and 200 mM Ce6 were applied (p < 0.05). The antimicrobial effects of PDI mediated by Ce6 for all single pathogenic biofilms were confirmed by live/dead staining under CLSM analysis. For all single-species biofilms, the use of PDI mediated by chlorin-e6 photosensitizer under blue or red-light irradiation (450 and 660 nm) demonstrated a significant reduction in bacterial viability, but blue light showed a promising higher photobiological effect, encouraging its adjuvant use to basic periodontitis treatment.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:31:11Z
2020-12-12T01:31:11Z
2020-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.pdpdt.2020.101916
Photodiagnosis and Photodynamic Therapy, v. 31.
1873-1597
1572-1000
http://hdl.handle.net/11449/199117
10.1016/j.pdpdt.2020.101916
2-s2.0-85088113623
url http://dx.doi.org/10.1016/j.pdpdt.2020.101916
http://hdl.handle.net/11449/199117
identifier_str_mv Photodiagnosis and Photodynamic Therapy, v. 31.
1873-1597
1572-1000
10.1016/j.pdpdt.2020.101916
2-s2.0-85088113623
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Photodiagnosis and Photodynamic Therapy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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