Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/JOURNAL.PNTD.0010791 http://hdl.handle.net/11449/249244 |
Resumo: | Trypanosoma vivax is a unicellular hemoparasite, and a principal cause of animal African trypanosomiasis (AAT), a vector-borne and potentially fatal livestock disease across sub-Saharan Africa. Previously, we identified diverse T. vivax-specific genes that were predicted to encode cell surface proteins. Here, we examine the immune responses of naturally and experimentally infected hosts to these unique parasite antigens, to identify immunogens that could become vaccine candidates. Immunoprofiling of host serum shows that one particular family (Fam34) elicits a consistent IgG antibody response. This gene family, which we now call Vivaxin, encodes at least 124 transmembrane glycoproteins that display quite distinct expression profiles and patterns of genetic variation. We focused on one gene (viv-β8) that encodes one particularly immunogenic vivaxin protein and which is highly expressed during infections but displays minimal polymorphism across the parasite population. Vaccination of mice with VIVβ8 adjuvanted with Quil-A elicits a strong, balanced immune response and delays parasite proliferation in some animals but, ultimately, it does not prevent disease. Although VIVβ8 is localized across the cell body and flagellar membrane, live immunostaining indicates that VIVβ8 is largely inaccessible to antibody in vivo. However, our phylogenetic analysis shows that vivaxin includes other antigens shown recently to induce immunity against T. vivax. Thus, the introduction of vivaxin represents an important advance in our understanding of the T. vivax cell surface. Besides being a source of proven and promising vaccine antigens, the gene family is clearly an important component of the parasite glycocalyx, with potential to influence host-parasite interactions. |
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Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surfaceTrypanosoma vivax is a unicellular hemoparasite, and a principal cause of animal African trypanosomiasis (AAT), a vector-borne and potentially fatal livestock disease across sub-Saharan Africa. Previously, we identified diverse T. vivax-specific genes that were predicted to encode cell surface proteins. Here, we examine the immune responses of naturally and experimentally infected hosts to these unique parasite antigens, to identify immunogens that could become vaccine candidates. Immunoprofiling of host serum shows that one particular family (Fam34) elicits a consistent IgG antibody response. This gene family, which we now call Vivaxin, encodes at least 124 transmembrane glycoproteins that display quite distinct expression profiles and patterns of genetic variation. We focused on one gene (viv-β8) that encodes one particularly immunogenic vivaxin protein and which is highly expressed during infections but displays minimal polymorphism across the parasite population. Vaccination of mice with VIVβ8 adjuvanted with Quil-A elicits a strong, balanced immune response and delays parasite proliferation in some animals but, ultimately, it does not prevent disease. Although VIVβ8 is localized across the cell body and flagellar membrane, live immunostaining indicates that VIVβ8 is largely inaccessible to antibody in vivo. However, our phylogenetic analysis shows that vivaxin includes other antigens shown recently to induce immunity against T. vivax. Thus, the introduction of vivaxin represents an important advance in our understanding of the T. vivax cell surface. Besides being a source of proven and promising vaccine antigens, the gene family is clearly an important component of the parasite glycocalyx, with potential to influence host-parasite interactions.Consejo Nacional de Ciencia, Tecnología e Innovación TecnológicaFondo Nacional de Desarrollo Científico y TecnológicoInstitute of Infection Veterinary and Ecological Sciences University of LiverpoolDepartment of Tropical Disease Biology Liverpool School of Tropical MedicineWellcome Trust Sanger Institute Wellcome Genome CampusDepartment of Biology Hull York Medical School York Biomedical Research Institute University of YorkDepartment of Pathology Reproduction and One Health Faculty of Agrarian and Veterinary Sciences São Paulo State University (UNESP), Sao PauloDepartment of Parasitology Institute of Biomedical Sciences University of Sao Paulo, Sao PauloSchool of Pharmacy and Life Sciences The Robert Gordon UniversityWaterford Institute of TechnologyDepartment of Pathology Reproduction and One Health Faculty of Agrarian and Veterinary Sciences São Paulo State University (UNESP), Sao PauloUniversity of LiverpoolLiverpool School of Tropical MedicineWellcome Genome CampusUniversity of YorkUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)The Robert Gordon UniversityWaterford Institute of TechnologyRomero-Ramirez, AlessandraCasas-Sánchez, AitorAutheman, DelphineDuffy, Craig W.Brandt, CordeliaClare, SimonHarcourt, KatherineAndré, Marcos Rogério [UNESP]Neto, Kayo José Garcia de Almeida Castilho [UNESP]Teixeira, Marta M. G.Machado, Rosangela Zacharias [UNESP]Coombes, JanineFlynn, Robin J.Wright, Gavin J.Jackson, Andrew P.2023-07-29T14:51:56Z2023-07-29T14:51:56Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/JOURNAL.PNTD.0010791PLoS Neglected Tropical Diseases, v. 16, n. 9, 2022.1935-27351935-2727http://hdl.handle.net/11449/24924410.1371/JOURNAL.PNTD.00107912-s2.0-85139570733Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS Neglected Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-06-07T13:01:54Zoai:repositorio.unesp.br:11449/249244Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-06-07T13:01:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface |
title |
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface |
spellingShingle |
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface Romero-Ramirez, Alessandra |
title_short |
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface |
title_full |
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface |
title_fullStr |
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface |
title_full_unstemmed |
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface |
title_sort |
Vivaxin genes encode highly immunogenic, non-variant antigens on the Trypanosoma vivax cell-surface |
author |
Romero-Ramirez, Alessandra |
author_facet |
Romero-Ramirez, Alessandra Casas-Sánchez, Aitor Autheman, Delphine Duffy, Craig W. Brandt, Cordelia Clare, Simon Harcourt, Katherine André, Marcos Rogério [UNESP] Neto, Kayo José Garcia de Almeida Castilho [UNESP] Teixeira, Marta M. G. Machado, Rosangela Zacharias [UNESP] Coombes, Janine Flynn, Robin J. Wright, Gavin J. Jackson, Andrew P. |
author_role |
author |
author2 |
Casas-Sánchez, Aitor Autheman, Delphine Duffy, Craig W. Brandt, Cordelia Clare, Simon Harcourt, Katherine André, Marcos Rogério [UNESP] Neto, Kayo José Garcia de Almeida Castilho [UNESP] Teixeira, Marta M. G. Machado, Rosangela Zacharias [UNESP] Coombes, Janine Flynn, Robin J. Wright, Gavin J. Jackson, Andrew P. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Liverpool Liverpool School of Tropical Medicine Wellcome Genome Campus University of York Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) The Robert Gordon University Waterford Institute of Technology |
dc.contributor.author.fl_str_mv |
Romero-Ramirez, Alessandra Casas-Sánchez, Aitor Autheman, Delphine Duffy, Craig W. Brandt, Cordelia Clare, Simon Harcourt, Katherine André, Marcos Rogério [UNESP] Neto, Kayo José Garcia de Almeida Castilho [UNESP] Teixeira, Marta M. G. Machado, Rosangela Zacharias [UNESP] Coombes, Janine Flynn, Robin J. Wright, Gavin J. Jackson, Andrew P. |
description |
Trypanosoma vivax is a unicellular hemoparasite, and a principal cause of animal African trypanosomiasis (AAT), a vector-borne and potentially fatal livestock disease across sub-Saharan Africa. Previously, we identified diverse T. vivax-specific genes that were predicted to encode cell surface proteins. Here, we examine the immune responses of naturally and experimentally infected hosts to these unique parasite antigens, to identify immunogens that could become vaccine candidates. Immunoprofiling of host serum shows that one particular family (Fam34) elicits a consistent IgG antibody response. This gene family, which we now call Vivaxin, encodes at least 124 transmembrane glycoproteins that display quite distinct expression profiles and patterns of genetic variation. We focused on one gene (viv-β8) that encodes one particularly immunogenic vivaxin protein and which is highly expressed during infections but displays minimal polymorphism across the parasite population. Vaccination of mice with VIVβ8 adjuvanted with Quil-A elicits a strong, balanced immune response and delays parasite proliferation in some animals but, ultimately, it does not prevent disease. Although VIVβ8 is localized across the cell body and flagellar membrane, live immunostaining indicates that VIVβ8 is largely inaccessible to antibody in vivo. However, our phylogenetic analysis shows that vivaxin includes other antigens shown recently to induce immunity against T. vivax. Thus, the introduction of vivaxin represents an important advance in our understanding of the T. vivax cell surface. Besides being a source of proven and promising vaccine antigens, the gene family is clearly an important component of the parasite glycocalyx, with potential to influence host-parasite interactions. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-07-29T14:51:56Z 2023-07-29T14:51:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/JOURNAL.PNTD.0010791 PLoS Neglected Tropical Diseases, v. 16, n. 9, 2022. 1935-2735 1935-2727 http://hdl.handle.net/11449/249244 10.1371/JOURNAL.PNTD.0010791 2-s2.0-85139570733 |
url |
http://dx.doi.org/10.1371/JOURNAL.PNTD.0010791 http://hdl.handle.net/11449/249244 |
identifier_str_mv |
PLoS Neglected Tropical Diseases, v. 16, n. 9, 2022. 1935-2735 1935-2727 10.1371/JOURNAL.PNTD.0010791 2-s2.0-85139570733 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS Neglected Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1826303779825254400 |