LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pcbi.1010081 http://hdl.handle.net/11449/240146 |
Resumo: | Ethanol alters many subsystems of Saccharomyces cerevisiae, including the cell cycle. Two ethanol-responsive lncRNAs in yeast interact with cell cycle proteins, and here, we investigated the role of these RNAs in cell cycle. Our network dynamic modeling showed that higher and lower ethanol-tolerant strains undergo cell cycle arrest in mitosis and G1 phases, respectively, during ethanol stress. The higher population rebound of the lower ethanol-tolerant phenotype after stress relief responds to the late phase arrest. We found that the lncRNA lnc9136 of SEY6210 (a lower ethanol-tolerant strain) induces cells to skip mitosis arrest. Simulating an overexpression of lnc9136 and analyzing CRISPR–Cas9 mutants lacking this lncRNA suggest that lnc9136 induces a regular cell cycle even under ethanol stress, indirectly regulating Swe1p and Clb1/2 by binding to Gin4p and Hsl1p. Notably, lnc10883 of BY4742 (a higher ethanol-tolerant strain) does not prevent G1 arrest in this strain under ethanol stress. However, lnc19883 circumvents DNA and spindle damage checkpoints, maintaining a functional cell cycle by interacting with Mec1p or Bub1p even in the presence of DNA/spindle damage. Overall, we present the first evidence of direct roles for lncRNAs in regulating yeast cell cycle proteins, the dynamics of this system in different ethanol-tolerant phenotypes, and a new yeast cell cycle model. |
id |
UNSP_afba800f1198f64358072664e021a1f4 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/240146 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stressEthanol alters many subsystems of Saccharomyces cerevisiae, including the cell cycle. Two ethanol-responsive lncRNAs in yeast interact with cell cycle proteins, and here, we investigated the role of these RNAs in cell cycle. Our network dynamic modeling showed that higher and lower ethanol-tolerant strains undergo cell cycle arrest in mitosis and G1 phases, respectively, during ethanol stress. The higher population rebound of the lower ethanol-tolerant phenotype after stress relief responds to the late phase arrest. We found that the lncRNA lnc9136 of SEY6210 (a lower ethanol-tolerant strain) induces cells to skip mitosis arrest. Simulating an overexpression of lnc9136 and analyzing CRISPR–Cas9 mutants lacking this lncRNA suggest that lnc9136 induces a regular cell cycle even under ethanol stress, indirectly regulating Swe1p and Clb1/2 by binding to Gin4p and Hsl1p. Notably, lnc10883 of BY4742 (a higher ethanol-tolerant strain) does not prevent G1 arrest in this strain under ethanol stress. However, lnc19883 circumvents DNA and spindle damage checkpoints, maintaining a functional cell cycle by interacting with Mec1p or Bub1p even in the presence of DNA/spindle damage. Overall, we present the first evidence of direct roles for lncRNAs in regulating yeast cell cycle proteins, the dynamics of this system in different ethanol-tolerant phenotypes, and a new yeast cell cycle model.Department of Parasitology Institute of Biomedical Sciences Sāo Paulo University (USP)Department of Bioprocess and Biotechnology School of Agriculture Sao Paulo State University (UNESP)Department of Structural and Functional Biology Institute of Bioscience at Botucatu Sao Paulo State University (UNESP)Max Planck Institute for Heart and Lung ResearchDepartment of Bioprocess and Biotechnology School of Agriculture Sao Paulo State University (UNESP)Department of Structural and Functional Biology Institute of Bioscience at Botucatu Sao Paulo State University (UNESP)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Max Planck Institute for Heart and Lung ResearchLázari, Lucas Cardoso [UNESP]Wolf, Ivan Rodrigo [UNESP]Schnepper, Amanda Piveta [UNESP]Valente, Guilherme Targino [UNESP]2023-03-01T20:03:26Z2023-03-01T20:03:26Z2022-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pcbi.1010081PLoS Computational Biology, v. 18, n. 5, 2022.1553-73581553-734Xhttp://hdl.handle.net/11449/24014610.1371/journal.pcbi.10100812-s2.0-85130851838Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS Computational Biologyinfo:eu-repo/semantics/openAccess2023-03-01T20:03:26Zoai:repositorio.unesp.br:11449/240146Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:01:41.605405Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress |
title |
LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress |
spellingShingle |
LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress Lázari, Lucas Cardoso [UNESP] |
title_short |
LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress |
title_full |
LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress |
title_fullStr |
LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress |
title_full_unstemmed |
LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress |
title_sort |
LncRNAs of Saccharomyces cerevisiae bypass the cell cycle arrest imposed by ethanol stress |
author |
Lázari, Lucas Cardoso [UNESP] |
author_facet |
Lázari, Lucas Cardoso [UNESP] Wolf, Ivan Rodrigo [UNESP] Schnepper, Amanda Piveta [UNESP] Valente, Guilherme Targino [UNESP] |
author_role |
author |
author2 |
Wolf, Ivan Rodrigo [UNESP] Schnepper, Amanda Piveta [UNESP] Valente, Guilherme Targino [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Max Planck Institute for Heart and Lung Research |
dc.contributor.author.fl_str_mv |
Lázari, Lucas Cardoso [UNESP] Wolf, Ivan Rodrigo [UNESP] Schnepper, Amanda Piveta [UNESP] Valente, Guilherme Targino [UNESP] |
description |
Ethanol alters many subsystems of Saccharomyces cerevisiae, including the cell cycle. Two ethanol-responsive lncRNAs in yeast interact with cell cycle proteins, and here, we investigated the role of these RNAs in cell cycle. Our network dynamic modeling showed that higher and lower ethanol-tolerant strains undergo cell cycle arrest in mitosis and G1 phases, respectively, during ethanol stress. The higher population rebound of the lower ethanol-tolerant phenotype after stress relief responds to the late phase arrest. We found that the lncRNA lnc9136 of SEY6210 (a lower ethanol-tolerant strain) induces cells to skip mitosis arrest. Simulating an overexpression of lnc9136 and analyzing CRISPR–Cas9 mutants lacking this lncRNA suggest that lnc9136 induces a regular cell cycle even under ethanol stress, indirectly regulating Swe1p and Clb1/2 by binding to Gin4p and Hsl1p. Notably, lnc10883 of BY4742 (a higher ethanol-tolerant strain) does not prevent G1 arrest in this strain under ethanol stress. However, lnc19883 circumvents DNA and spindle damage checkpoints, maintaining a functional cell cycle by interacting with Mec1p or Bub1p even in the presence of DNA/spindle damage. Overall, we present the first evidence of direct roles for lncRNAs in regulating yeast cell cycle proteins, the dynamics of this system in different ethanol-tolerant phenotypes, and a new yeast cell cycle model. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01 2023-03-01T20:03:26Z 2023-03-01T20:03:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pcbi.1010081 PLoS Computational Biology, v. 18, n. 5, 2022. 1553-7358 1553-734X http://hdl.handle.net/11449/240146 10.1371/journal.pcbi.1010081 2-s2.0-85130851838 |
url |
http://dx.doi.org/10.1371/journal.pcbi.1010081 http://hdl.handle.net/11449/240146 |
identifier_str_mv |
PLoS Computational Biology, v. 18, n. 5, 2022. 1553-7358 1553-734X 10.1371/journal.pcbi.1010081 2-s2.0-85130851838 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS Computational Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128741758468096 |