Bioactive Secondary Metabolites from Phomopsis sp., an Endophytic Fungus from Senna spectabilis

• Autor(a) principal: Chapla, Vanessa Mara [UNESP]
• Data de Publicação: 2014
• Outros Autores: Zeraik, Maria Luiza [UNESP], Ximenes, Valdecir F. [UNESP], Zanardi, Lisineia Maria [UNESP], Lopes, Márcia Nasser [UNESP], Cavalheiro, Alberto José [UNESP], Silva, Dulce Helena Siqueira [UNESP], Young, Maria Claudia M., Fonseca, Luiz Marcos da [UNESP], Bolzani, Vanderlan da Silva [UNESP], Araújo, Angela Regina [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.3390/molecules19056597; http://hdl.handle.net/11449/113198
• topic: secondary metabolites; bioactivities; endophytic fungi; Phomopsis sp.; Senna spectabilis

Chemical investigation of an acetonitrile fraction from the endophytic fungus Phomopsis sp. led to the isolation of the new natural product 2-hydroxy-alternariol (7) together with the known compounds cytochalasins J (1) and H (2), 5'-epialtenuene (3) and the mycotoxins alternariol monomethyl ether (AME, 4), alternariol (AOH, 5) and cytosporone C (6). The structure of the new compound was elucidated by using 1-D and 2-D NMR (nuclear magnetic resonance) and high resolution mass spectrometry. The cytochalasins J (1) and H (2) and AOH (5) exhibited potent inhibition of the total ROS (reactive oxygen species) produced by stimulated human neutrophils and acted as potent potential anti-inflammatory agents. Moreover, cytochalasin H (2) demonstrated antifungal and acetylcholinesterase enzyme (AChE) inhibition in vitro.