Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro study

Detalhes bibliográficos
Autor(a) principal: Barroso, Rosmeire Aparecida
Data de Publicação: 2020
Outros Autores: Navarro, Ricardo, Tim, Carla Roberta, de Paula Ramos, Lucas [UNESP], de Oliveira, Luciane Dias [UNESP], Araki, Ângela Toshie, Fernandes, Karina Gonzales Camara, Macedo, Daniela, Assis, Lívia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10103-020-03163-3
http://hdl.handle.net/11449/205333
Resumo: Acne vulgaris is the most recurring skin condition in the world, causing great harm to the physical and psychological well-being of many patients. Antimicrobial photodynamic therapy (aPDT) has broad therapeutic applicability. The purpose was to evaluate in vitro the photodynamic inactivation against Propionibacterium acnes (P. acnes) biofilms by using different concentrations of hypericin (Hypericum perforatum) photosensitizer associated with different energies of low-level laser. The biofilms were placed in 96-well microplates with a 6.4-mm diameter surface, by using standard suspensions (2 × 107 CFU/mL) and grown in brain heart infusion broth (BHI) for 48 h in anaerobic chamber. Subsequently, the control group received application of 0.9% sterile saline solution for 3 min; the photosensitising groups received hypericin at concentrations of 5 and 15 μg/mL for 3 min; the laser groups received irradiation of energies of 3 and 5 J (660 nm, continuous output, 100 mW, 30 and 50 s and 100 J/cm2 and 166 J/cm2, respectively); the aPDT groups received 5 and 15 μg/mL concentrations of hypericin associated with energies of 3 and 5 J of low-level laser irradiation. After the biofilms were broken up and seeded for CFU counting. The results showed a reduction in P. acnes biofilms after aPDT emphasising that 15 μg/mL hypericin associated with 3 and 5 J laser irradiation reduced biofilms by 14.1 and 27.9%, respectively. In addition, all groups of aPDT demostrated statistically significant reductions. In vitro photodynamic inactivation against P. acnes biofilms using different concentration of hypericin photosensitizer associated with different energies of low-level laser promoted effective antimicrobial action.
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spelling Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro studyAcne vulgarisAntimicrobial photodynamic therapyHypericinPhotochemotherapyPhotosensitizing agents, PropionibacteriumAcne vulgaris is the most recurring skin condition in the world, causing great harm to the physical and psychological well-being of many patients. Antimicrobial photodynamic therapy (aPDT) has broad therapeutic applicability. The purpose was to evaluate in vitro the photodynamic inactivation against Propionibacterium acnes (P. acnes) biofilms by using different concentrations of hypericin (Hypericum perforatum) photosensitizer associated with different energies of low-level laser. The biofilms were placed in 96-well microplates with a 6.4-mm diameter surface, by using standard suspensions (2 × 107 CFU/mL) and grown in brain heart infusion broth (BHI) for 48 h in anaerobic chamber. Subsequently, the control group received application of 0.9% sterile saline solution for 3 min; the photosensitising groups received hypericin at concentrations of 5 and 15 μg/mL for 3 min; the laser groups received irradiation of energies of 3 and 5 J (660 nm, continuous output, 100 mW, 30 and 50 s and 100 J/cm2 and 166 J/cm2, respectively); the aPDT groups received 5 and 15 μg/mL concentrations of hypericin associated with energies of 3 and 5 J of low-level laser irradiation. After the biofilms were broken up and seeded for CFU counting. The results showed a reduction in P. acnes biofilms after aPDT emphasising that 15 μg/mL hypericin associated with 3 and 5 J laser irradiation reduced biofilms by 14.1 and 27.9%, respectively. In addition, all groups of aPDT demostrated statistically significant reductions. In vitro photodynamic inactivation against P. acnes biofilms using different concentration of hypericin photosensitizer associated with different energies of low-level laser promoted effective antimicrobial action.Scientific and Technological Institute Biomedical Engineering Graduate Program Universidade BrasilInstitute of Science and Technology Department of Biosciences and Oral Diagnosis São Paulo State University (UNESP)Dentistry Graduate Program Universidade Cruzeiro do SulInstituto Científico e Tecnológico da Universidade Brasil Programa de Pós Graduação em Engenharia Biomédica Universidade Brasil, Carolina Fonseca 236Institute of Science and Technology Department of Biosciences and Oral Diagnosis São Paulo State University (UNESP)Universidade BrasilUniversidade Estadual Paulista (Unesp)Universidade Cruzeiro do SulBarroso, Rosmeire AparecidaNavarro, RicardoTim, Carla Robertade Paula Ramos, Lucas [UNESP]de Oliveira, Luciane Dias [UNESP]Araki, Ângela ToshieFernandes, Karina Gonzales CamaraMacedo, DanielaAssis, Lívia2021-06-25T10:13:33Z2021-06-25T10:13:33Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10103-020-03163-3Lasers in Medical Science.1435-604X0268-8921http://hdl.handle.net/11449/20533310.1007/s10103-020-03163-32-s2.0-85092773677Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Scienceinfo:eu-repo/semantics/openAccess2021-10-23T12:39:34Zoai:repositorio.unesp.br:11449/205333Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:50:20.301875Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro study
title Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro study
spellingShingle Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro study
Barroso, Rosmeire Aparecida
Acne vulgaris
Antimicrobial photodynamic therapy
Hypericin
Photochemotherapy
Photosensitizing agents, Propionibacterium
title_short Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro study
title_full Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro study
title_fullStr Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro study
title_full_unstemmed Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro study
title_sort Antimicrobial photodynamic therapy against Propionibacterium acnes biofilms using hypericin (Hypericum perforatum) photosensitizer: in vitro study
author Barroso, Rosmeire Aparecida
author_facet Barroso, Rosmeire Aparecida
Navarro, Ricardo
Tim, Carla Roberta
de Paula Ramos, Lucas [UNESP]
de Oliveira, Luciane Dias [UNESP]
Araki, Ângela Toshie
Fernandes, Karina Gonzales Camara
Macedo, Daniela
Assis, Lívia
author_role author
author2 Navarro, Ricardo
Tim, Carla Roberta
de Paula Ramos, Lucas [UNESP]
de Oliveira, Luciane Dias [UNESP]
Araki, Ângela Toshie
Fernandes, Karina Gonzales Camara
Macedo, Daniela
Assis, Lívia
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Brasil
Universidade Estadual Paulista (Unesp)
Universidade Cruzeiro do Sul
dc.contributor.author.fl_str_mv Barroso, Rosmeire Aparecida
Navarro, Ricardo
Tim, Carla Roberta
de Paula Ramos, Lucas [UNESP]
de Oliveira, Luciane Dias [UNESP]
Araki, Ângela Toshie
Fernandes, Karina Gonzales Camara
Macedo, Daniela
Assis, Lívia
dc.subject.por.fl_str_mv Acne vulgaris
Antimicrobial photodynamic therapy
Hypericin
Photochemotherapy
Photosensitizing agents, Propionibacterium
topic Acne vulgaris
Antimicrobial photodynamic therapy
Hypericin
Photochemotherapy
Photosensitizing agents, Propionibacterium
description Acne vulgaris is the most recurring skin condition in the world, causing great harm to the physical and psychological well-being of many patients. Antimicrobial photodynamic therapy (aPDT) has broad therapeutic applicability. The purpose was to evaluate in vitro the photodynamic inactivation against Propionibacterium acnes (P. acnes) biofilms by using different concentrations of hypericin (Hypericum perforatum) photosensitizer associated with different energies of low-level laser. The biofilms were placed in 96-well microplates with a 6.4-mm diameter surface, by using standard suspensions (2 × 107 CFU/mL) and grown in brain heart infusion broth (BHI) for 48 h in anaerobic chamber. Subsequently, the control group received application of 0.9% sterile saline solution for 3 min; the photosensitising groups received hypericin at concentrations of 5 and 15 μg/mL for 3 min; the laser groups received irradiation of energies of 3 and 5 J (660 nm, continuous output, 100 mW, 30 and 50 s and 100 J/cm2 and 166 J/cm2, respectively); the aPDT groups received 5 and 15 μg/mL concentrations of hypericin associated with energies of 3 and 5 J of low-level laser irradiation. After the biofilms were broken up and seeded for CFU counting. The results showed a reduction in P. acnes biofilms after aPDT emphasising that 15 μg/mL hypericin associated with 3 and 5 J laser irradiation reduced biofilms by 14.1 and 27.9%, respectively. In addition, all groups of aPDT demostrated statistically significant reductions. In vitro photodynamic inactivation against P. acnes biofilms using different concentration of hypericin photosensitizer associated with different energies of low-level laser promoted effective antimicrobial action.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
2021-06-25T10:13:33Z
2021-06-25T10:13:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10103-020-03163-3
Lasers in Medical Science.
1435-604X
0268-8921
http://hdl.handle.net/11449/205333
10.1007/s10103-020-03163-3
2-s2.0-85092773677
url http://dx.doi.org/10.1007/s10103-020-03163-3
http://hdl.handle.net/11449/205333
identifier_str_mv Lasers in Medical Science.
1435-604X
0268-8921
10.1007/s10103-020-03163-3
2-s2.0-85092773677
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lasers in Medical Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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