Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2014/840613 http://hdl.handle.net/11449/117242 |
Resumo: | The effects of six soft liners (Ufi Gel P (UG), Sofreliner S (SR), Durabase Soft (D), Trusoft (T), Coe Comfort (CC), and Softone (ST)) on L929, HaCat, and RAW 264.7 cells were investigated. Eluates (24 and 48 h) from the materials were applied on the cells and the viability, type of cell death, and morphology were evaluated. Cells were also seeded on the specimens' surfaces (direct contact) and incubated (24 or 48 h), and viability was analyzed. Controls were cells in culture medium without eluates or specimens. For cell viability, no significant differences were found among materials or between extraction periods, and the liners were noncytotoxic or slightly cytotoxic. Morphology of RAW 264.7 cells was altered by the 24 h eluates from CC and D and the 48 h eluates from SR, CC, and D. The 24 and 48 h eluates from all materials (except T) increased the percentages of L929 necrotic cells. For direct contact tests, the lowest cytotoxicity was observed for UG and SR. Although eluates did not reduce viability, morphology alterations and increase in necrosis were seen. Moreover, in the direct contact, effects on viability were more pronounced, particularly for D, T, CC and ST. Thus, the use of UG and SR might reduce the risk of adverse effects. |
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Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 MacrophagesThe effects of six soft liners (Ufi Gel P (UG), Sofreliner S (SR), Durabase Soft (D), Trusoft (T), Coe Comfort (CC), and Softone (ST)) on L929, HaCat, and RAW 264.7 cells were investigated. Eluates (24 and 48 h) from the materials were applied on the cells and the viability, type of cell death, and morphology were evaluated. Cells were also seeded on the specimens' surfaces (direct contact) and incubated (24 or 48 h), and viability was analyzed. Controls were cells in culture medium without eluates or specimens. For cell viability, no significant differences were found among materials or between extraction periods, and the liners were noncytotoxic or slightly cytotoxic. Morphology of RAW 264.7 cells was altered by the 24 h eluates from CC and D and the 48 h eluates from SR, CC, and D. The 24 and 48 h eluates from all materials (except T) increased the percentages of L929 necrotic cells. For direct contact tests, the lowest cytotoxicity was observed for UG and SR. Although eluates did not reduce viability, morphology alterations and increase in necrosis were seen. Moreover, in the direct contact, effects on viability were more pronounced, particularly for D, T, CC and ST. Thus, the use of UG and SR might reduce the risk of adverse effects.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Univ Estadual Paulista, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, UNESP, BR-14801903 Araraquara, SP, BrazilUniv Estadual Paulista, Araraquara Dent Sch, Dept Physiol & Pathol, UNESP, BR-14801903 Araraquara, SP, BrazilUniv Estadual Paulista, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, UNESP, BR-14801903 Araraquara, SP, BrazilUniv Estadual Paulista, Araraquara Dent Sch, Dept Physiol & Pathol, UNESP, BR-14801903 Araraquara, SP, BrazilFAPESP: 10/08750-0FAPESP: 10/11764-3FAPESP: 12/07994-9CNPq: 302543/2010-4Hindawi Publishing CorporationUniversidade Estadual Paulista (Unesp)Lima Chaves, Carolina de Andrade [UNESP]Souza Costa, Carlos Alberto de [UNESP]Vergani, Carlos Eduardo [UNESP]Chaves de Souza, Pedro Paulo [UNESP]Machado, Ana Lucia [UNESP]2015-03-18T15:55:37Z2015-03-18T15:55:37Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/epub+zipapplication/pdfhttp://dx.doi.org/10.1155/2014/840613Biomed Research International. New York: Hindawi Publishing Corporation, 14 p., 2014.2314-6133http://hdl.handle.net/11449/11724210.1155/2014/840613WOS:000346014700001WOS000346014700001.pdfWOS000346014700001.epub4517484241515548300313052242782080002487818425870000-0002-7375-4714Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomed Research International2.5830,935info:eu-repo/semantics/openAccess2023-12-01T06:13:31Zoai:repositorio.unesp.br:11449/117242Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-01T06:13:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages |
title |
Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages |
spellingShingle |
Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages Lima Chaves, Carolina de Andrade [UNESP] |
title_short |
Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages |
title_full |
Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages |
title_fullStr |
Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages |
title_full_unstemmed |
Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages |
title_sort |
Effects of Soft Denture Liners on L929 Fibroblasts, HaCaT Keratinocytes, and RAW 264.7 Macrophages |
author |
Lima Chaves, Carolina de Andrade [UNESP] |
author_facet |
Lima Chaves, Carolina de Andrade [UNESP] Souza Costa, Carlos Alberto de [UNESP] Vergani, Carlos Eduardo [UNESP] Chaves de Souza, Pedro Paulo [UNESP] Machado, Ana Lucia [UNESP] |
author_role |
author |
author2 |
Souza Costa, Carlos Alberto de [UNESP] Vergani, Carlos Eduardo [UNESP] Chaves de Souza, Pedro Paulo [UNESP] Machado, Ana Lucia [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lima Chaves, Carolina de Andrade [UNESP] Souza Costa, Carlos Alberto de [UNESP] Vergani, Carlos Eduardo [UNESP] Chaves de Souza, Pedro Paulo [UNESP] Machado, Ana Lucia [UNESP] |
description |
The effects of six soft liners (Ufi Gel P (UG), Sofreliner S (SR), Durabase Soft (D), Trusoft (T), Coe Comfort (CC), and Softone (ST)) on L929, HaCat, and RAW 264.7 cells were investigated. Eluates (24 and 48 h) from the materials were applied on the cells and the viability, type of cell death, and morphology were evaluated. Cells were also seeded on the specimens' surfaces (direct contact) and incubated (24 or 48 h), and viability was analyzed. Controls were cells in culture medium without eluates or specimens. For cell viability, no significant differences were found among materials or between extraction periods, and the liners were noncytotoxic or slightly cytotoxic. Morphology of RAW 264.7 cells was altered by the 24 h eluates from CC and D and the 48 h eluates from SR, CC, and D. The 24 and 48 h eluates from all materials (except T) increased the percentages of L929 necrotic cells. For direct contact tests, the lowest cytotoxicity was observed for UG and SR. Although eluates did not reduce viability, morphology alterations and increase in necrosis were seen. Moreover, in the direct contact, effects on viability were more pronounced, particularly for D, T, CC and ST. Thus, the use of UG and SR might reduce the risk of adverse effects. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 2015-03-18T15:55:37Z 2015-03-18T15:55:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2014/840613 Biomed Research International. New York: Hindawi Publishing Corporation, 14 p., 2014. 2314-6133 http://hdl.handle.net/11449/117242 10.1155/2014/840613 WOS:000346014700001 WOS000346014700001.pdf WOS000346014700001.epub 4517484241515548 3003130522427820 8000248781842587 0000-0002-7375-4714 |
url |
http://dx.doi.org/10.1155/2014/840613 http://hdl.handle.net/11449/117242 |
identifier_str_mv |
Biomed Research International. New York: Hindawi Publishing Corporation, 14 p., 2014. 2314-6133 10.1155/2014/840613 WOS:000346014700001 WOS000346014700001.pdf WOS000346014700001.epub 4517484241515548 3003130522427820 8000248781842587 0000-0002-7375-4714 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomed Research International 2.583 0,935 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 application/epub+zip application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965130325229568 |