Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021)
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s42770-022-00891-2 http://hdl.handle.net/11449/248097 |
Resumo: | Central nervous system (CNS) infections comprise life-threatening clinical conditions in domestic species, and are commonly related to severe sequelae, disability, or high fatality rates. A set of bacterial pathogens have been identified in central nervous infections in livestock and companion animals, although the most of descriptions are restricted to case reports and a lack of comprehensive studies involving CNS-related bacterial infections have been focused on a great number of domestic species. In this scenario, we retrospectively investigated selected epidemiological data, clinical findings, bacteriological culture, and in vitro susceptibility patterns of 136 nonrepetitive neurologic cases in domestic species (2005–2021). Bacterial isolates were recovered from 25% (34/136) of the cerebrospinal fluid (CSF) sampled. The isolates were obtained from cattle (9/136 = 6.6%), dogs (7/136 = 5.1%), horses (6/136 = 4.4%), goats (3/136 = 2.2%), pigs (3/136 = 2.2%), sheep (3/136 = 2.2%), cats (2/136 = 1.5%), and asinine (1/136 = 0.7%). Among animals with bacterial isolation, Staphylococcus aureus (6/34 = 17.6%), Escherichia coli (5/34 = 14.7%), Staphylococcus beta-hemolytic (5/34 = 14.7%), and Trueperella pyogenes (3/34 = 8.8%) were predominant, in addition to a miscellaneous of other bacteria isolated in minor frequency, e.g., Corynebacterium pseudotuberculosis, Enterobacter cloacae, Mannheimia haemolytica, Pseudomonas aeruginosa, and Streptococcus equi subsp. equi. In vitro susceptibility tests of isolates revealed that amoxicillin/clavulanic acid (11/13 = 84.6%), cephalexin (9/11 = 81.8%), and florfenicol (9/12 = 75%) were the most effective antimicrobials. Conversely, isolates exhibited resistance mainly to tetracycline (6/10 = 60%), penicillin (6/11 = 54.5%), and trimethoprim/sulfamethoxazole (5/11 = 45.5%). Also, multidrug resistance to ≥ 3 classes of antimicrobials was found in 23.5% (8/34) strains. Data relative to the outcome was available in 79.4% (27/34) of animals that had bacterial isolation, and from these, the lethality rate was 92.6% (25/27). Incoordination (14/34 = 41.2%), recumbency (11/34 = 32.4%), apathy (10/34 = 29.4%), anorexia (9/34 = 26.5%), blindness (7/34 = 20.6%), seizure (6/34 = 17.6%), limb paresis (5/34 = 14.7%), head-pressing (4/34 = 11.8%), and nystagmus (3/34 = 8.8%) were the most frequent clinical signs. A variety of bacterial pathogens were identified in the CSF of domestic species showing neurologic signs, with a predominance of staphylococci, streptococci, and enterobacteria. High lethality of cases, poor in vitro efficacy of conventional antimicrobials, and a high in vitro multidrug resistance pattern of isolates were seen. Our results contribute to etiological characterization, antimicrobial resistance patterns, and clinical-epidemiological findings of bacterial infections in domestic species with neurological signs. |
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Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021)Bacterial etiologyCentral nervous infectionsCompanion animalsLivestockMultidrug resistanceCentral nervous system (CNS) infections comprise life-threatening clinical conditions in domestic species, and are commonly related to severe sequelae, disability, or high fatality rates. A set of bacterial pathogens have been identified in central nervous infections in livestock and companion animals, although the most of descriptions are restricted to case reports and a lack of comprehensive studies involving CNS-related bacterial infections have been focused on a great number of domestic species. In this scenario, we retrospectively investigated selected epidemiological data, clinical findings, bacteriological culture, and in vitro susceptibility patterns of 136 nonrepetitive neurologic cases in domestic species (2005–2021). Bacterial isolates were recovered from 25% (34/136) of the cerebrospinal fluid (CSF) sampled. The isolates were obtained from cattle (9/136 = 6.6%), dogs (7/136 = 5.1%), horses (6/136 = 4.4%), goats (3/136 = 2.2%), pigs (3/136 = 2.2%), sheep (3/136 = 2.2%), cats (2/136 = 1.5%), and asinine (1/136 = 0.7%). Among animals with bacterial isolation, Staphylococcus aureus (6/34 = 17.6%), Escherichia coli (5/34 = 14.7%), Staphylococcus beta-hemolytic (5/34 = 14.7%), and Trueperella pyogenes (3/34 = 8.8%) were predominant, in addition to a miscellaneous of other bacteria isolated in minor frequency, e.g., Corynebacterium pseudotuberculosis, Enterobacter cloacae, Mannheimia haemolytica, Pseudomonas aeruginosa, and Streptococcus equi subsp. equi. In vitro susceptibility tests of isolates revealed that amoxicillin/clavulanic acid (11/13 = 84.6%), cephalexin (9/11 = 81.8%), and florfenicol (9/12 = 75%) were the most effective antimicrobials. Conversely, isolates exhibited resistance mainly to tetracycline (6/10 = 60%), penicillin (6/11 = 54.5%), and trimethoprim/sulfamethoxazole (5/11 = 45.5%). Also, multidrug resistance to ≥ 3 classes of antimicrobials was found in 23.5% (8/34) strains. Data relative to the outcome was available in 79.4% (27/34) of animals that had bacterial isolation, and from these, the lethality rate was 92.6% (25/27). Incoordination (14/34 = 41.2%), recumbency (11/34 = 32.4%), apathy (10/34 = 29.4%), anorexia (9/34 = 26.5%), blindness (7/34 = 20.6%), seizure (6/34 = 17.6%), limb paresis (5/34 = 14.7%), head-pressing (4/34 = 11.8%), and nystagmus (3/34 = 8.8%) were the most frequent clinical signs. A variety of bacterial pathogens were identified in the CSF of domestic species showing neurologic signs, with a predominance of staphylococci, streptococci, and enterobacteria. High lethality of cases, poor in vitro efficacy of conventional antimicrobials, and a high in vitro multidrug resistance pattern of isolates were seen. Our results contribute to etiological characterization, antimicrobial resistance patterns, and clinical-epidemiological findings of bacterial infections in domestic species with neurological signs.Department of Animal Production and Preventive Veterinary Medicine Faculty of Veterinary Medicine and Animal Sciences - FMVZ Sao Paulo State University - UNESP, SPSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SPSchool of Veterinary Medicine University of Western São Paulo - UNOESTE, SPSchool of Veterinary Medicine Midwestern State University - UNICENTRO, PRDepartment of Animal Production and Preventive Veterinary Medicine Faculty of Veterinary Medicine and Animal Sciences - FMVZ Sao Paulo State University - UNESP, SPSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)University of Western São Paulo - UNOESTEMidwestern State University - UNICENTRORibeiro, Márcio Garcia [UNESP]Pereira, Thiago Tourinho [UNESP]de Lima Paz, Patrik Júnior [UNESP]de Almeida, Beatriz Oliveira [UNESP]Cerviño, Carmen S. Araújo [UNESP]Rodrigues, Carolina Aparecida [UNESP]Santos, Gabrielly Terra Sartori [UNESP]de Souza Freire, Larissa Maria [UNESP]Portilho, Fábio Vinicius Ramos [UNESP]Filho, Marcelo Fagali Árabe [UNESP]Paschoal, Natália Rodrigues [UNESP]Bello, Thaís Spessotto [UNESP]Megid, Jane [UNESP]Langoni, Helio [UNESP]Appolinário, Camila Michele [UNESP]Borges, Alexandre Secorun [UNESP]Amorim, Rogério Martins [UNESP]Giuffrida, Rogériode Oliveira Filho, José Paes [UNESP]Siqueira, Amanda KellerListoni, Fernando José Paganini [UNESP]Paes, Antonio Carlos [UNESP]2023-07-29T13:34:21Z2023-07-29T13:34:21Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article449-457http://dx.doi.org/10.1007/s42770-022-00891-2Brazilian Journal of Microbiology, v. 54, n. 1, p. 449-457, 2023.1678-44051517-8382http://hdl.handle.net/11449/24809710.1007/s42770-022-00891-22-s2.0-85144874629Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiologyinfo:eu-repo/semantics/openAccess2023-07-29T13:34:22Zoai:repositorio.unesp.br:11449/248097Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:34:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021) |
title |
Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021) |
spellingShingle |
Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021) Ribeiro, Márcio Garcia [UNESP] Bacterial etiology Central nervous infections Companion animals Livestock Multidrug resistance |
title_short |
Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021) |
title_full |
Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021) |
title_fullStr |
Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021) |
title_full_unstemmed |
Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021) |
title_sort |
Bacterial identification in cerebrospinal fluid of domestic species with neurologic signs: a retrospective case-series study in 136 animals (2005–2021) |
author |
Ribeiro, Márcio Garcia [UNESP] |
author_facet |
Ribeiro, Márcio Garcia [UNESP] Pereira, Thiago Tourinho [UNESP] de Lima Paz, Patrik Júnior [UNESP] de Almeida, Beatriz Oliveira [UNESP] Cerviño, Carmen S. Araújo [UNESP] Rodrigues, Carolina Aparecida [UNESP] Santos, Gabrielly Terra Sartori [UNESP] de Souza Freire, Larissa Maria [UNESP] Portilho, Fábio Vinicius Ramos [UNESP] Filho, Marcelo Fagali Árabe [UNESP] Paschoal, Natália Rodrigues [UNESP] Bello, Thaís Spessotto [UNESP] Megid, Jane [UNESP] Langoni, Helio [UNESP] Appolinário, Camila Michele [UNESP] Borges, Alexandre Secorun [UNESP] Amorim, Rogério Martins [UNESP] Giuffrida, Rogério de Oliveira Filho, José Paes [UNESP] Siqueira, Amanda Keller Listoni, Fernando José Paganini [UNESP] Paes, Antonio Carlos [UNESP] |
author_role |
author |
author2 |
Pereira, Thiago Tourinho [UNESP] de Lima Paz, Patrik Júnior [UNESP] de Almeida, Beatriz Oliveira [UNESP] Cerviño, Carmen S. Araújo [UNESP] Rodrigues, Carolina Aparecida [UNESP] Santos, Gabrielly Terra Sartori [UNESP] de Souza Freire, Larissa Maria [UNESP] Portilho, Fábio Vinicius Ramos [UNESP] Filho, Marcelo Fagali Árabe [UNESP] Paschoal, Natália Rodrigues [UNESP] Bello, Thaís Spessotto [UNESP] Megid, Jane [UNESP] Langoni, Helio [UNESP] Appolinário, Camila Michele [UNESP] Borges, Alexandre Secorun [UNESP] Amorim, Rogério Martins [UNESP] Giuffrida, Rogério de Oliveira Filho, José Paes [UNESP] Siqueira, Amanda Keller Listoni, Fernando José Paganini [UNESP] Paes, Antonio Carlos [UNESP] |
author2_role |
author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Western São Paulo - UNOESTE Midwestern State University - UNICENTRO |
dc.contributor.author.fl_str_mv |
Ribeiro, Márcio Garcia [UNESP] Pereira, Thiago Tourinho [UNESP] de Lima Paz, Patrik Júnior [UNESP] de Almeida, Beatriz Oliveira [UNESP] Cerviño, Carmen S. Araújo [UNESP] Rodrigues, Carolina Aparecida [UNESP] Santos, Gabrielly Terra Sartori [UNESP] de Souza Freire, Larissa Maria [UNESP] Portilho, Fábio Vinicius Ramos [UNESP] Filho, Marcelo Fagali Árabe [UNESP] Paschoal, Natália Rodrigues [UNESP] Bello, Thaís Spessotto [UNESP] Megid, Jane [UNESP] Langoni, Helio [UNESP] Appolinário, Camila Michele [UNESP] Borges, Alexandre Secorun [UNESP] Amorim, Rogério Martins [UNESP] Giuffrida, Rogério de Oliveira Filho, José Paes [UNESP] Siqueira, Amanda Keller Listoni, Fernando José Paganini [UNESP] Paes, Antonio Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Bacterial etiology Central nervous infections Companion animals Livestock Multidrug resistance |
topic |
Bacterial etiology Central nervous infections Companion animals Livestock Multidrug resistance |
description |
Central nervous system (CNS) infections comprise life-threatening clinical conditions in domestic species, and are commonly related to severe sequelae, disability, or high fatality rates. A set of bacterial pathogens have been identified in central nervous infections in livestock and companion animals, although the most of descriptions are restricted to case reports and a lack of comprehensive studies involving CNS-related bacterial infections have been focused on a great number of domestic species. In this scenario, we retrospectively investigated selected epidemiological data, clinical findings, bacteriological culture, and in vitro susceptibility patterns of 136 nonrepetitive neurologic cases in domestic species (2005–2021). Bacterial isolates were recovered from 25% (34/136) of the cerebrospinal fluid (CSF) sampled. The isolates were obtained from cattle (9/136 = 6.6%), dogs (7/136 = 5.1%), horses (6/136 = 4.4%), goats (3/136 = 2.2%), pigs (3/136 = 2.2%), sheep (3/136 = 2.2%), cats (2/136 = 1.5%), and asinine (1/136 = 0.7%). Among animals with bacterial isolation, Staphylococcus aureus (6/34 = 17.6%), Escherichia coli (5/34 = 14.7%), Staphylococcus beta-hemolytic (5/34 = 14.7%), and Trueperella pyogenes (3/34 = 8.8%) were predominant, in addition to a miscellaneous of other bacteria isolated in minor frequency, e.g., Corynebacterium pseudotuberculosis, Enterobacter cloacae, Mannheimia haemolytica, Pseudomonas aeruginosa, and Streptococcus equi subsp. equi. In vitro susceptibility tests of isolates revealed that amoxicillin/clavulanic acid (11/13 = 84.6%), cephalexin (9/11 = 81.8%), and florfenicol (9/12 = 75%) were the most effective antimicrobials. Conversely, isolates exhibited resistance mainly to tetracycline (6/10 = 60%), penicillin (6/11 = 54.5%), and trimethoprim/sulfamethoxazole (5/11 = 45.5%). Also, multidrug resistance to ≥ 3 classes of antimicrobials was found in 23.5% (8/34) strains. Data relative to the outcome was available in 79.4% (27/34) of animals that had bacterial isolation, and from these, the lethality rate was 92.6% (25/27). Incoordination (14/34 = 41.2%), recumbency (11/34 = 32.4%), apathy (10/34 = 29.4%), anorexia (9/34 = 26.5%), blindness (7/34 = 20.6%), seizure (6/34 = 17.6%), limb paresis (5/34 = 14.7%), head-pressing (4/34 = 11.8%), and nystagmus (3/34 = 8.8%) were the most frequent clinical signs. A variety of bacterial pathogens were identified in the CSF of domestic species showing neurologic signs, with a predominance of staphylococci, streptococci, and enterobacteria. High lethality of cases, poor in vitro efficacy of conventional antimicrobials, and a high in vitro multidrug resistance pattern of isolates were seen. Our results contribute to etiological characterization, antimicrobial resistance patterns, and clinical-epidemiological findings of bacterial infections in domestic species with neurological signs. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:34:21Z 2023-07-29T13:34:21Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s42770-022-00891-2 Brazilian Journal of Microbiology, v. 54, n. 1, p. 449-457, 2023. 1678-4405 1517-8382 http://hdl.handle.net/11449/248097 10.1007/s42770-022-00891-2 2-s2.0-85144874629 |
url |
http://dx.doi.org/10.1007/s42770-022-00891-2 http://hdl.handle.net/11449/248097 |
identifier_str_mv |
Brazilian Journal of Microbiology, v. 54, n. 1, p. 449-457, 2023. 1678-4405 1517-8382 10.1007/s42770-022-00891-2 2-s2.0-85144874629 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
449-457 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964490424385536 |