Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12906-018-2281-y http://hdl.handle.net/11449/176618 |
Resumo: | Background: Propolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (μTBS) of the dentin/resin interface. Methods: An ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 μL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the μTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b*chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05). Results: The UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean μTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3. Conclusions: The EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or μTBS of the dentin/resin interface. |
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Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agentAntibacterial activityCavity disinfectantDental cariesDental fillingsIsoflavonoidsMicellar nanocompositesRed propolisUPLC-DAD assayBackground: Propolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (μTBS) of the dentin/resin interface. Methods: An ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 μL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the μTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b*chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05). Results: The UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean μTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3. Conclusions: The EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or μTBS of the dentin/resin interface.Cesmac University Center Postgraduate Program in Health Research, Rua Cônego Machado, 825, FarolFederal University of Alagoas Campus AC Simões Department of Restorative Dentistry Faculty of Dentistry, Av. Lourival Melo Mota, S/N, Tabuleiro do MartinsFederal University of Alagoas Campus A. C Laboratory of Quality Control of Drugs and Medicines Postgraduate Program in Pharmaceutical Sciences School of Nursing and Pharmacy, SimõesFederal University of Alagoas Campus A. C Laboratory of Applied Electrochemistry Institute of Chemistry and Biotechnology, SimõesFederal University of Alagoas Campus AC Simões Department of Cariology Faculty of Dentistry, Av. Lourival Melo Mota, S/N, Tabuleiro do MartinsUniversidade Estadual Paulista Júlio de Mesquita Filho-UNESP Department of Dental Materials and Prosthodontics Faculty of Dentistry, AraraquaraFederal University of Alagoas Campus Arapiraca Faculty of Veterinary Medicine, Unit of ViçosaFederal University of Alagoas Campus A. C Laboratory of Bacteriology Institute of Biological and Health Sciences, SimõesUniversidade Estadual Paulista Júlio de Mesquita Filho-UNESP Department of Dental Materials and Prosthodontics Faculty of Dentistry, AraraquaraPostgraduate Program in Health ResearchFaculty of DentistrySchool of Nursing and PharmacyInstitute of Chemistry and BiotechnologyUniversidade Estadual Paulista (Unesp)Faculty of Veterinary MedicineInstitute of Biological and Health SciencesCelerino de Moraes Porto, Isabel CristinaChaves Cardoso de Almeida, DayseVasconcelos Calheiros de Oliveira Costa, GabrielaSampaio Donato, Tayná StéphanieMoreira Nunes, LetíciaGomes do Nascimento, Ticianodos Santos Oliveira, José MarcosBatista da Silva, CarolinaBarbosa dos Santos, Natanaelde Alencar e Silva Leite, Maria Luísa [UNESP]Diniz Basílio-Júnior, IrinaldoBraga Dornelas, CamilaBarnabé Escodro, Pierreda Silva Fonseca, Eduardo JorgeUmeko Kamiya, Regianne2018-12-11T17:21:45Z2018-12-11T17:21:45Z2018-07-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12906-018-2281-yBMC Complementary and Alternative Medicine, v. 18, n. 1, 2018.1472-6882http://hdl.handle.net/11449/17661810.1186/s12906-018-2281-y2-s2.0-850503066542-s2.0-85050306654.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Complementary and Alternative Medicine0,858info:eu-repo/semantics/openAccess2023-10-22T06:10:14Zoai:repositorio.unesp.br:11449/176618Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:38:32.318988Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent |
title |
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent |
spellingShingle |
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent Celerino de Moraes Porto, Isabel Cristina Antibacterial activity Cavity disinfectant Dental caries Dental fillings Isoflavonoids Micellar nanocomposites Red propolis UPLC-DAD assay |
title_short |
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent |
title_full |
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent |
title_fullStr |
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent |
title_full_unstemmed |
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent |
title_sort |
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent |
author |
Celerino de Moraes Porto, Isabel Cristina |
author_facet |
Celerino de Moraes Porto, Isabel Cristina Chaves Cardoso de Almeida, Dayse Vasconcelos Calheiros de Oliveira Costa, Gabriela Sampaio Donato, Tayná Stéphanie Moreira Nunes, Letícia Gomes do Nascimento, Ticiano dos Santos Oliveira, José Marcos Batista da Silva, Carolina Barbosa dos Santos, Natanael de Alencar e Silva Leite, Maria Luísa [UNESP] Diniz Basílio-Júnior, Irinaldo Braga Dornelas, Camila Barnabé Escodro, Pierre da Silva Fonseca, Eduardo Jorge Umeko Kamiya, Regianne |
author_role |
author |
author2 |
Chaves Cardoso de Almeida, Dayse Vasconcelos Calheiros de Oliveira Costa, Gabriela Sampaio Donato, Tayná Stéphanie Moreira Nunes, Letícia Gomes do Nascimento, Ticiano dos Santos Oliveira, José Marcos Batista da Silva, Carolina Barbosa dos Santos, Natanael de Alencar e Silva Leite, Maria Luísa [UNESP] Diniz Basílio-Júnior, Irinaldo Braga Dornelas, Camila Barnabé Escodro, Pierre da Silva Fonseca, Eduardo Jorge Umeko Kamiya, Regianne |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Postgraduate Program in Health Research Faculty of Dentistry School of Nursing and Pharmacy Institute of Chemistry and Biotechnology Universidade Estadual Paulista (Unesp) Faculty of Veterinary Medicine Institute of Biological and Health Sciences |
dc.contributor.author.fl_str_mv |
Celerino de Moraes Porto, Isabel Cristina Chaves Cardoso de Almeida, Dayse Vasconcelos Calheiros de Oliveira Costa, Gabriela Sampaio Donato, Tayná Stéphanie Moreira Nunes, Letícia Gomes do Nascimento, Ticiano dos Santos Oliveira, José Marcos Batista da Silva, Carolina Barbosa dos Santos, Natanael de Alencar e Silva Leite, Maria Luísa [UNESP] Diniz Basílio-Júnior, Irinaldo Braga Dornelas, Camila Barnabé Escodro, Pierre da Silva Fonseca, Eduardo Jorge Umeko Kamiya, Regianne |
dc.subject.por.fl_str_mv |
Antibacterial activity Cavity disinfectant Dental caries Dental fillings Isoflavonoids Micellar nanocomposites Red propolis UPLC-DAD assay |
topic |
Antibacterial activity Cavity disinfectant Dental caries Dental fillings Isoflavonoids Micellar nanocomposites Red propolis UPLC-DAD assay |
description |
Background: Propolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (μTBS) of the dentin/resin interface. Methods: An ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 μL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the μTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b*chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05). Results: The UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean μTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3. Conclusions: The EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or μTBS of the dentin/resin interface. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:21:45Z 2018-12-11T17:21:45Z 2018-07-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12906-018-2281-y BMC Complementary and Alternative Medicine, v. 18, n. 1, 2018. 1472-6882 http://hdl.handle.net/11449/176618 10.1186/s12906-018-2281-y 2-s2.0-85050306654 2-s2.0-85050306654.pdf |
url |
http://dx.doi.org/10.1186/s12906-018-2281-y http://hdl.handle.net/11449/176618 |
identifier_str_mv |
BMC Complementary and Alternative Medicine, v. 18, n. 1, 2018. 1472-6882 10.1186/s12906-018-2281-y 2-s2.0-85050306654 2-s2.0-85050306654.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Complementary and Alternative Medicine 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128544004374528 |